The sliding motility of the bacilliform virions of Influenza A viruses

Stevens, Laurie; Buyl, Sophie de; Mognetti, Bortolo Matteo

doi:10.1039/d3sm00371j

Cited by 2 publications

(2 citation statements)

References 56 publications

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“…We model the virus as positioned at a fixed elevation above a 1D substrate uniformly decorated with SA at an average spacing l ∼ 2.5 nm reflecting typical glycoprotein densities. HA binding to SA is described as a bimolecular reaction with the off-rate ( k off ) from empirical measurements [27], and the on-rate ( k on ) estimated by modeling HA trimers and SA as confined complexes following previous studies [15, 23]. NA irreversibly cleaves HA-bound SA via first-order kinetics with a catalytic rate k c [28].…”

Section: Modelmentioning

confidence: 99%

“…Experiments on coverslips functionalized with sialic acid (SA) showed persistent translational motion of the virions away from the NA-rich pole, operating via a ‘burnt-bridge’ Brownian ratchet mechanism [22]. Recent work [23] has revealed that this motion is modulated by the HA/NA activity and their spatial organization, with observed differences in transport speeds and gaits, though the explored parameter space was limited. In contrast, influenza C viruses exhibit rolling motion on SA-functionalized surfaces due to the coordinated SA binding/cleavage cycle of their hemagglutinin-esterase-fusion glycoprotein [24], which imposes a uniform surface distribution of binders & cleavers.…”

Section: Introductionmentioning

confidence: 99%

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The kinetics and optimality of influenza A virus locomotion

Agarwal,

Veytsman,

Fletcher

et al. 2024

Preprint

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Influenza A viruses (IAVs) must navigate through a dense extracellular mucus to infect airway epithelial cells. The mucous layer, composed of glycosylated biopolymers (mucins), presents sialic acid that binds to ligands on the viral envelope and can be irreversibly cleaved by viral enzymes. It was recently discovered that filamentous IAVs exhibit directed, persistent motion along their long axis on sialic acid-coated surfaces. This study demonstrates, through stochastic simulations and mean-field theory, how IAVs harness a 'burnt-bridge' Brownian ratchet mechanism for directed, persistent translational motion. We show viruses occupy the optimal parameter range ('Goldilocks zone') for efficient mucous transport, possibly due to the evolutionary adaptation of enzyme kinetics. Our findings suggest novel therapeutic targets and provide insight into possible mechanisms of zoonotic transmission.

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Section: Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The kinetics and optimality of influenza A virus locomotion

Agarwal,

Veytsman,

Fletcher

et al. 2024

Preprint

View full text Add to dashboard Cite

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Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR

Abreu,

Royer,

Poitevin

et al. 2023

Viruses

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After the Coronavirus pandemic, the importance of virus surveillance was highlighted, reinforcing the constant necessity of discussing and updating the methods for collection and diagnoses, including for other respiratory viruses. Although the nasopharyngeal swab is the gold-standard sample for detecting and genotyping SARS-CoV-2 and Influenza viruses, its collection is uncomfortable and requires specialized teams, which can be costly. During the pandemic, non-invasive saliva samples proved to be a suitable alternative for SARS-CoV-2 diagnosis, but for Influenza virus the use of this sample source is not recognized yet. In addition, most SARS-CoV-2 comparisons were conducted before the Omicron variant emerged. Here, we aimed to compare Influenza A and Omicron RT-qPCR analysis of nasopharyngeal swabs and saliva self-collection in paired samples from 663 individuals. We found that both nasopharyngeal swab and saliva collection are efficient for the diagnosis of Omicron (including sub-lineages) and for Influenza A, with high sensitivity and accuracy (>90%). The kappa index is 0.938 for Influenza A and 0.905 for SARS-CoV-2. These results showed excellent agreement between the two samples reinforcing saliva samples as a reliable source for detecting Omicron and highlighting saliva as a valid sample source for Influenza detection, considering this cheaper and more comfortable alternative.

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The sliding motility of the bacilliform virions of Influenza A viruses

Abstract: Influenza A virus (IAV) infection relies on the action of the hemagglutinin (HA) and neuraminidase (NA) membrane proteins. The HA ligands anchor the IAV virion to the cell's surface by...

Cited by 2 publications

References 56 publications

The kinetics and optimality of influenza A virus locomotion

The kinetics and optimality of influenza A virus locomotion

Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR

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