The skin microbiome in allergen‐induced canine atopic dermatitis

Pierezan, Felipe; Olivry, Thierry; Paps, Judith S; Lawhon, Sara D.; Wu, Jing; Steiner, Jörg M.; Suchodolski, Jan S.; Hoffmann, Aline Rodrigues

doi:10.1111/vde.12366

Cited by 66 publications

(72 citation statements)

References 20 publications

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“…The skin microbiota also was longitudinally evaluated in a canine model of AD using HDM challenges . This experimental study included eight dogs that were hypersensitized with HDM and when challenged on the right inguinal area with HDM, they developed focal skin lesions.…”

Section: Dysbiosis Of the Skin Microbiome Is Associated With Skin Dismentioning

confidence: 99%

“…The skin microbiota also was longitudinally evaluated in a canine model of AD using HDM challenges. 91 This experimental study included eight dogs that were hypersensitized with HDM and when challenged on the right inguinal area with HDM, they developed focal skin lesions. Samples were collected prior to and after challenges from the affected and contralateral areas, and changes in the microbiota were analysed longitudinally.…”

Section: Dysbiosis Of the Skin Microbiome Is Associated With Skin Dismentioning

confidence: 99%

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The cutaneous ecosystem: the roles of the skin microbiome in health and its association with inflammatory skin conditions in humans and animals

Hoffmann

2017

Veterinary Dermatology

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Section: Dysbiosis Of the Skin Microbiome Is Associated With Skin Dismentioning

confidence: 99%

Section: Dysbiosis Of the Skin Microbiome Is Associated With Skin Dismentioning

confidence: 99%

The cutaneous ecosystem: the roles of the skin microbiome in health and its association with inflammatory skin conditions in humans and animals

Hoffmann

2017

Veterinary Dermatology

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“…With the help of metagenomic Next‐Generation sequencing (NGS) of the bacterial 16S ribosomal RNA (rRNA) genes, a large number of uncommon or previously uncultured bacteria on human and canine skin have been discovered . In both humans and dogs with AD, a reduction in bacterial diversity has been noted …”

Section: Introductionmentioning

confidence: 99%

Ear canal microbiota – a comparison between healthy dogs and atopic dogs without clinical signs of otitis externa

Ngo

Taminiau

Fall³

et al. 2018

Veterinary Dermatology

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“…In canine atopic dermatitis, Staphylococcus pseudintermedius has been classically associated with the disease. Microbiota studies of canine atopic dermatitis presented an overrepresentation of Staphylococcus genus [13, 14], but the species was confirmed when complementing the studies using directed qPCRs for the species of interest [13] or using a Staphylococcus -specific database and V1-V3 region amplification [14].…”

Section: Introductionmentioning

confidence: 99%

Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and whole rrn operon

Cuscó¹,

Catozzi

Viñes

et al. 2018

Preprint

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Background. Profiling microbiome on low biomass samples is challenging for metagenomics since these samples are prone to present DNA from other sources, such as the host or the environment. The usual approach is sequencing specific hypervariable regions of the 16S rRNA gene, which fails to assign taxonomy to genus and species level. Here, we aim to assess longamplicon PCR-based approaches for assigning taxonomy at the genus and species level. We use Nanopore sequencing with two different markers: full-length 16S rRNA (~1,500 bp) and the whole rrn operon (16S rRNA gene -ITS -23S rRNA gene; 4,500 bp). Methods.We sequenced a clinical isolate of Staphylococcus pseudintermedius, two mock communities (HM-783D, Bei Resources; D6306, ZymoBIOMICS™) and two pools of lowbiomass samples (dog skin). Nanopore sequencing was performed on MinION™ (Oxford Nanopore Technologies) using 1D PCR barcoding kit. Sequences were pre-processed, and data were analyzed using WIMP workflow on EPI2ME (ONT) or Minimap2 software with rrn database.Results. Full-length 16S rRNA and the rrn operon retrieved the microbiota composition from the bacterial isolate, the mock communities and the complex skin samples, even at the genus and species level. For Staphylococcus pseudintermedius isolate, when using EPI2ME, the amplicons were assigned to the correct bacterial species in ~98% of the cases with rrn operon as the marker, and ~68% of the cases with 16S rRNA gene respectively. In both skin microbiota samples, we detected many species with an environmental origin. In chin, we found different Pseudomonas species in high abundance, whereas in dorsal skin there were more taxa with lower abundances.Conclusions. Both full-length 16S rRNA and the rrn operon retrieved the microbiota composition of simple and complex microbial communities, even from the low-biomass samples such as dog skin. For an increased resolution at the species level, rrn operon would be the best choice.

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The skin microbiome in allergen‐induced canine atopic dermatitis

Abstract: This study demonstrates that an allergen challenge in sensitized dogs leads to bacterial dysbiosis with increased abundance of S. pseudintermedius at the site of lesion induction.

Cited by 66 publications

References 20 publications

The cutaneous ecosystem: the roles of the skin microbiome in health and its association with inflammatory skin conditions in humans and animals

The cutaneous ecosystem: the roles of the skin microbiome in health and its association with inflammatory skin conditions in humans and animals

Ear canal microbiota – a comparison between healthy dogs and atopic dogs without clinical signs of otitis externa

Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and whole rrn operon

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