The skin barrier state of aged hairless mice in a dry environment

Choi, Eung Ho; Kim, M.J.; Ahn, Sung Ku; Park, W.S.; Son, Eui Dong; Nam, Gnu; Chang, Ih-Seop; Lee, S.H.

doi:10.1046/j.1365-2133.2002.04918.x

Cited by 17 publications

(6 citation statements)

References 20 publications

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“…The only significant difference detected was the TEWL between ESS with mDPC-GFP and host murine skin. Very low TEWL in skin of athymic mice has been reported previously [15]. These results suggested no compromise of skin integrity in either of the ESS models.…”

Section: Discussionsupporting

confidence: 78%

Characterization of Hair Follicle Development in Engineered Skin Substitutes

et al. 2013

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Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human keratinocytes from neonatal foreskins and cultured murine dermal papilla cells from adult GFP transgenic mice and grafted orthotopically to full-thickness wounds on athymic mice. Non-cultured dissociated neonatal murine-only skin cells, or cultured human-only skin keratinocytes and fibroblasts without dermal papilla cells served as positive and negative controls respectively. In this study, neonatal murine-only skin substitutes formed external hairs and sebaceous glands, chimeric skin substitutes formed pigmented hairs without sebaceous glands, and human-only skin substitutes formed no follicles or glands. Although chimeric hair cannot erupt readily, removal of upper skin layer exposed keratinized hair shafts at the skin surface. Development of incomplete pilosebaceous units in chimeric hair corresponded with upregulation of hair-related genes, LEF1 and WNT10B, and downregulation of a marker of sebaceous glands, Steroyl-CoA desaturase. Transepidermal water loss was normal in all conditions. This study demonstrated that while sebaceous glands may be involved in hair eruption, they are not required for hair development in engineered skin substitutes.

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Section: Discussionsupporting

confidence: 78%

Characterization of Hair Follicle Development in Engineered Skin Substitutes

et al. 2013

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“…Epidermal Ca 2+ has been shown to coordinately regulate both lamellar body secretion and terminal differentiation (i.e. cornified envelope formation, loss of cellular organelles and synthesis of differentiation‐specific proteins) 29–31 . In addition, epidermal terminal differentiation is also directed by activation of caspase 14, a cysteinyl aspartate‐specific proteinase (reviewed in Denecker et al.…”

Section: Resultsmentioning

confidence: 99%

“…cornified envelope formation, loss of cellular organelles and synthesis of differentiation-specific proteins). [29][30][31] In addition, epidermal terminal differentiation is also directed by activation of caspase 14, a cysteinyl aspartate-specific proteinase (reviewed in Denecker et al 32 ). To determine which processes are controlled specifically by ER Ca 2+ emptying, we treated normal hairless mice with a single topical application of a low concentration (100 nmol L )1 ) of TG, applied to unperturbed flank skin.…”

Section: Thapsigargin Treatment Of Intact Skin Does Not Perturb the Pmentioning

confidence: 99%

Endoplasmic reticulum Ca2+ depletion activates XBP1 and controls terminal differentiation in keratinocytes and epidermis

Celli

Mackenzie

Crumrine

et al. 2010

British Journal of Dermatology

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SUMMARYBackground-Endoplasmic reticulum (ER) Ca2+ depletion, previously shown to signal pathologic stress responses, has more recently been found also to trigger homeostatic physiologic processes such as differentiation. In keratinocytes and epidermis, terminal differentiation and barrier repair require physiologic apoptosis and differentiation, as evidenced by protein synthesis, caspase 14 expression, lipid secretion, and stratum corneum (SC) formation.

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“…Stratum corneum hydration and TEWL were measured using Corneometer CM825 and Tewameter TM300 (Courage and Khazaka Electronics, Cologne, Germany) at 3 weeks and 6 weeks. 27,28) Probes were applied to the skin surfaces of mice. After the 6-week feeding period, mice were deprived of food overnight and anesthetized with diethyl ether.…”

Section: Methodsmentioning

confidence: 99%

Dietary Phospholipid Concentrate from Bovine Milk Improves Epidermal Function in Hairless Mice

Haruta¹,

Kato²,

Yoshioka³

2008

Bioscience, Biotechnology, and Biochemistry

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We investigated the effect of dietary phospholipid (PL) concentrate from bovine milk on the epidermis. Thirteen-week-old hairless male and female mice (Hos:HR-1) were separated into two experimental groups, each fed two experimental diets: the control group and the PL group. The mice were given the experimental diets for 6 weeks. Stratum corneum hydration and transepidermal water loss (TEWL) were measured using Corneometer CM825 and Tewameter TM300 (Courage and Khazaka Electronics, Cologne, Germany) at 3 weeks and 6 weeks. After the feeding period, ceramides in stratum corneum were analyzed. We found that stratum corneum hydration and ceramides in the PL group were significantly higher than those in the control group and that TEWL in the PL group tended to decrease.These results indicate that dietary PL concentrate improves epidermal function by increasing the amount of ceramides, resulting in higher hydration.

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The skin barrier state of aged hairless mice in a dry environment

Abstract: We confirmed that a dry environment induces epidermal proliferation and scaling in both aged and young mice. However, no remarkable difference was found in the skin barrier recovery of aged hairless mice in a dry environment.

Cited by 17 publications

References 20 publications

Characterization of Hair Follicle Development in Engineered Skin Substitutes

Characterization of Hair Follicle Development in Engineered Skin Substitutes

Endoplasmic reticulum Ca2+ depletion activates XBP1 and controls terminal differentiation in keratinocytes and epidermis

Dietary Phospholipid Concentrate from Bovine Milk Improves Epidermal Function in Hairless Mice

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