The skewing to Th1 induced by lentinan is directed through the distinctive cytokine production by macrophages with elevated intracellular glutathione content

Murata, Yukie; Shimamura, Tetsuo; Tagami, Tomoyuki; Takatsuki, Fumihiko; Hamuro, Junji

doi:10.1016/s1567-5769(01)00212-0

Cited by 97 publications

(61 citation statements)

References 60 publications

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“…Previous studies have reported that -1,3-D-glucan modulated the Th1 and/or the Th2 cell response in experimental animals and human patients of allergic rhinitis and digestive cancers [14,25,49]. Murata et al reported that -1,3-Dglucan/lentinan skewed to Th1/Th2 balance to Th1 and that the skewing to Th1 by lentinan was directed through the distinctive production of IL-12 versus IL-6, IL-10 and prostaglandin E 2 by macrophages, depending on the intracellular glutathione redox status [25].…”

Section: Discussionmentioning

confidence: 99%

“…Murata et al reported that -1,3-Dglucan/lentinan skewed to Th1/Th2 balance to Th1 and that the skewing to Th1 by lentinan was directed through the distinctive production of IL-12 versus IL-6, IL-10 and prostaglandin E 2 by macrophages, depending on the intracellular glutathione redox status [25]. In the present study, paramylon suppressed both Th1 and Th2 cell responses, which is one of the novel effects of -1,3-D-glucan treatment on the immune response in vivo.…”

Section: Discussionmentioning

confidence: 99%

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Oral Administration of Paramylon, a .BETA.-1,3-D-Glucan Isolated from Euglena gracilis Z Inhibits Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Sugiyama

Hata

Suzuki³

et al. 2010

J. Vet. Med. Sci.

101

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ABSTRACT. Paramylon is a -1,3-D-glucan isolated from Euglena gracilis Z. This study was designed to evaluate the suppressive effects of the oral administration of paramylon on the development of atopic dermatitis (AD)-like skin lesions induced by repeated application of 2,4,6-trinitrochlorobenzene (TNCB) in sensitized NC/Nga mice. The effects of paramylon were assessed by measuring macroscopical and histopathological findings of skin, ear swelling, serum levels of total IgE, interleukin-4 (IL-4) and interferon- (IFN-) and IL-18 and IL-12 contents in the skin lesions. Oral administration of paramylon inhibited the development of AD-like skin lesions as exemplified by a significant decrease in dermatitis scores for the back, ear swelling and hypertrophy of the skin, infiltration of inflammatory cells in the skin, and serum IgE levels. Oral administration of paramylon reduced serum levels of both IL-4 and IFN- and IL-18 and IL-12 contents in the skin lesions. Oral administration of paramylon did not cause weight loss, as was observed with prednisolone. These results suggest that paramylon inhibits the development of AD-like skin lesions in NC/Nga mice by suppressing both the T-helper (Th) 1 and Th 2 cell responses. Our results indicate that paramylon treatment could provide an effective alternative therapy for the management of AD.KEY WORDS: atopic dermatitis, -1,3-D-glucan, interferon-, interleukin-4, paramylon.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Oral Administration of Paramylon, a .BETA.-1,3-D-Glucan Isolated from Euglena gracilis Z Inhibits Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Sugiyama

Hata

Suzuki³

et al. 2010

J. Vet. Med. Sci.

101

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“…In pre-clinical studies, the balance of type 1/2 T cell responses has been shown to be critically important in anti-tumor immune responses, such as the inhibition of tumor growth and metastasis, and survival rate. [27][28][29] It has been reported that immunomodulators, such as lipoteichoic-acid-related molecule from OK-432, 30) 1→3-b-D-glucan, 31,32) CpG-DNA, 33) glycyrrhizin from licorice roots, 34) and Celosia argentea seed extracts 35) can regulate the type 1/2 T cell responses from the type 2-dominant to type 1-dominant. In addition, it has been clinically reported that administration of lentinan, anti-tumor polysaccharides, 36) and OK-432 37) to patients with digestive cancer were able to improve T cell responses from the type 2-dominant state to the normal state in cancer patients.…”

Section: Discussionmentioning

confidence: 99%

Z-100, an Immunomodulatory Arabinomannan Extracted from Mycobacterium tuberculosis Strain Aoyama B, Augments Anti-tumor Activities of X-Ray Irradiation against B16 Melanoma in Association with the Improvement of Type 1T Cell Responses

Oka

Sasaki

Shiraishi

et al. 2004

Biological & Pharmaceutical Bulletin

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In this study, the effects of combination therapy consisting of X-ray irradiation and Z-100 on the survival time of C57BL/6 mice inoculated with B16F10 melanoma were investigated. Survival time was significantly prolonged in B16F10 melanoma-bearing mice treated with the X-ray irradiation (5 Gy) and Z-100 (10 mg/kg s.c.) combination therapy compared with mice irradiated with X-rays alone. The weight of primary tumors and number of metastatic colonies were also significantly suppressed by the combination therapy compared with that in the X-ray irradiation group. These results indicated that Z-100 could enhance the anti-tumor effects of radiotherapy against B16F10 melanoma. On the other hand, the survival time of CD4 knockout mice bearing the same tumors was not prolonged by the combination therapy compared with mice irradiated with X-rays alone, suggesting that CD4؉ cells are partly involved in augmentation of the anti-tumor effect of radiotherapy by Z-100. In addition, type 1 cytokine (IL-2, IFN-g g) production was significantly increased and type 2 cytokine (IL-4, IL-10) production was significantly suppressed in the tumor-bearing mice treated with the combination therapy compared with the X-ray irradiation group. Moreover, interleukin-12 production by CD11c؉ cells was also significantly increased in mice treated with the combination therapy compared with the X-ray irradiation group. These results indicate that Z-100 augmented the anti-tumor effects of X-ray irradiation. Moreover, we demonstrated that the effects of Z-100 were expressed at least in part, by the improvement of the T cell responses from type 2-dominant to type 1-dominant via up-regulation of IL-12 production.

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“…Furthermore, exposure of the macrophage to IFN-g tends to raise its GSH, thereby reinforcing its orientation towards type 1 activity. Conversely, exposure of the macrophage to IL-4 lowers its GSH and steers it towards type 2 activity [27]. From these observations, it can be concluded that the antioxidant status at the level of APCs and their microenvironment can influence the ultimate pattern of immune response.…”

Section: The Effect Of the Local Crh-mast Cell-histamine Axismentioning

confidence: 97%

Stress, Epigenetics and Thyroid Autoimmunity

Tsatsoulis

2009

The Epigenetics of Autoimmune Diseases

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The skewing to Th1 induced by lentinan is directed through the distinctive cytokine production by macrophages with elevated intracellular glutathione content

Cited by 97 publications

References 60 publications

Oral Administration of Paramylon, a .BETA.-1,3-D-Glucan Isolated from Euglena gracilis Z Inhibits Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Oral Administration of Paramylon, a .BETA.-1,3-D-Glucan Isolated from Euglena gracilis Z Inhibits Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Z-100, an Immunomodulatory Arabinomannan Extracted from Mycobacterium tuberculosis Strain Aoyama B, Augments Anti-tumor Activities of X-Ray Irradiation against B16 Melanoma in Association with the Improvement of Type 1T Cell Responses

Stress, Epigenetics and Thyroid Autoimmunity

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