The Size of <scp>DNA</scp> Molecules and Chromatin Organization in the Macronucleus of the Ciliate <i>Didinium nasutum</i> (Ciliophora)

Попенко, В. И.; Потехин, А. А.; Karajan, Bella P.; Skarlato, Sergei; Леонова, О.Г.

doi:10.1111/jeu.12161

Cited by 6 publications

(5 citation statements)

References 27 publications

(48 reference statements)

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“…S1 , Supplementary Material online). However, previous work using pulsed-field gel electrophoresis of total gDNA from D. nasutum did not report chromosomes <50 kb ( Popenko et al. 2015 ), which suggests that the nano-chromsomes may be present at relatively low copy numbers and/or that the retention of these chromosomes is strongly dependent on the DNA isolation approaches.…”

Section: Resultsmentioning

confidence: 89%

Twisted Tales: Insights into Genome Diversity of Ciliates Using Single-Cell ‘Omics

Maurer‐Alcalá

Yan

Pilling

et al. 2018

Genome Biology and Evolution

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The emergence of robust single-cell ‘omics techniques enables studies of uncultivable species, allowing for the (re)discovery of diverse genomic features. In this study, we combine single-cell genomics and transcriptomics to explore genome evolution in ciliates (a > 1 Gy old clade). Analysis of the data resulting from these single-cell ‘omics approaches show: 1) the description of the ciliates in the class Karyorelictea as “primitive” is inaccurate because their somatic macronuclei contain loci of varying copy number (i.e., they have been processed by genome rearrangements from the zygotic nucleus); 2) gene-sized somatic chromosomes exist in the class Litostomatea, consistent with Balbiani’s (1890) observation of giant chromosomes in this lineage; and 3) gene scrambling exists in the underexplored Postciliodesmatophora (the classes Heterotrichea and Karyorelictea, abbreviated here as the Po-clade), one of two major clades of ciliates. Together these data highlight the complex evolutionary patterns underlying germline genome architectures in ciliates and provide a basis for further exploration of principles of genome evolution in diverse microbial lineages.

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Section: Resultsmentioning

confidence: 89%

Twisted Tales: Insights into Genome Diversity of Ciliates Using Single-Cell ‘Omics

Maurer‐Alcalá

Yan

Pilling

et al. 2018

Genome Biology and Evolution

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“…However, our data indicate that the small size and the absence of typical chromosomes cannot be the only factors determining the inverted spatial organization of some ciliate nucleoli. Indeed, all three ciliate species studied here refer to the species with macronuclear DNA of subchromosomal size (Popenko et al, 1998(Popenko et al, , 2015Rautian and Potekhin, 2002). However, only in one of them we observed the unusual location of the fibrillar component and fibrillarin at the periphery of the nucleoli.…”

Section: Discussionmentioning

confidence: 99%

Localization of fibrillarin in the nucleoli of ciliate species with subchromosomal organization of macronuclear genome

Леонова¹,

Karajan²,

Skarlato³

et al. 2017

Protistology

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SummaryFibrillarin is one of the highly evolutionary conserved nucleolar proteins. It plays an important role in the early processing and modifcation of pre-rRNA, ribosome assembly, maintenance of nuclear shape and cellular growth, and is essential for the early embryonic development. During the interphase, fibrillarin is found in nucleoli of higher eukaryotes, mainly in fibrillar centers and dense fibrillar components as well as in the Cajal bodies. At present, little is known about distribution of nucleolar proteins in ciliates. In this work, Bursaria truncatella, Paramecium multimicronucleatum and Didinium nasutum were studied. These ciliates belong to the species with subchromosomal organization of macronuclear genome. The aim of this work was to study localization of fibrillarin in their nucleoli using confocal microscopy and specific antibodies. It was shown that in the nucleoli of B. truncatella and P. multimicronucleatum fibrillarin mainly occurs in the central region of the nucleoli. In D. nasutum, on the contrary, nucleoli fibrillarin was located at the periphery of the nucleolar domain. These results are in good agreement with the electron microscopic data on morphology of the nucleoli that show an unusual, "inverted" location of the fibrillar component and the granular component in the nucleoli of D. nasutum.

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“…After 4 days the cells were collected by centrifugation, washed in distilled water and fixed in 2.5% glutaraldehyde. Aliquotes (80 mkL) of cell suspensions were centrifuged in the small centrifuge chambers onto freshly glow-discharged carbon-collodion electron microscopic grids at 2500 g for 20 min [64,65]. Specimens were briefly rinsed in distilled water, stained for 1 min in 1% aqueous uranyl acetate solution [66], air dried and studied on a JEM-100CX electron microscope (JEOL, Peabody, MA, USA) in Core Facility “UNIQEM Collection” of Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences.…”

Section: Methodsmentioning

confidence: 99%

Novel 5′-Norcarbocyclic Pyrimidine Derivatives as Antibacterial Agents

et al. 2018

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A series of novel 5′-norcarbocyclic derivatives of 5-alkoxymethyl or 5-alkyltriazolyl-methyl uracil were synthesized and the activity of the compounds evaluated against both Gram-positive and Gram-negative bacteria. The growth of Mycobacterium smegmatis was completely inhibited by the most active compounds at a MIC99 of 67 μg/mL (mc2155) and a MIC99 of 6.7–67 μg/mL (VKPM Ac 1339). Several compounds also showed the ability to inhibit the growth of attenuated strains of Mycobacterium tuberculosis ATCC 25177 (MIC99 28–61 μg/mL) and Mycobacterium bovis ATCC 35737 (MIC99 50–60 μg/mL), as well as two virulent strains of M. tuberculosis; a laboratory strain H37Rv (MIC99 20–50 μg/mL) and a clinical strain with multiple drug resistance MS-115 (MIC99 20–50 μg/mL). Transmission electron microscopy (TEM) evaluation of M. tuberculosis H37Rv bacterial cells treated with one of the compounds demonstrated destruction of the bacterial cell wall, suggesting that the mechanism of action for these compounds may be related to their interactions with bacteria cell walls.

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The Size of DNA Molecules and Chromatin Organization in the Macronucleus of the Ciliate Didinium nasutum (Ciliophora)

Cited by 6 publications

References 27 publications

Twisted Tales: Insights into Genome Diversity of Ciliates Using Single-Cell ‘Omics

Twisted Tales: Insights into Genome Diversity of Ciliates Using Single-Cell ‘Omics

Localization of fibrillarin in the nucleoli of ciliate species with subchromosomal organization of macronuclear genome

Novel 5′-Norcarbocyclic Pyrimidine Derivatives as Antibacterial Agents

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