The Size of AOT Reverse Micelles

Eskici, Gözde; Axelsen, Paul H.

doi:10.1021/acs.jpcb.6b06420

Cited by 51 publications

(87 citation statements)

References 57 publications

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“…The results were shown in Figure 2 and 3. Results showed that in the extraction process, the reaction time was linearly related to 2 3…”

Section: Effect Of Temperature On Protein Extraction Ratementioning

confidence: 99%

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Study on Kinetics of Protein Forward Extraction from Quinoa by AOT/ Isooctane Reverse Micelles System

Wu¹,

Sun²,

Shi³

et al. 2018

Proceedings of the 2018 3rd International Conference on Modelling, Simulation and Applied Mathematics (MSAM 2018)

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In this paper, the kinetic process of extracting total protein from quinoa by AOT/ isooctane reverse micelles was systematically studied. The effects of extraction time, extraction temperature, particle size and oscillation rate on quinoa protein forward extraction rate were investigated respectively. Results showed that extraction of quinoa protein by AOT/ isooctane reverse micelles system was mainly controlled by internal diffusion, that is, the diffusion of protein from the interior of the particle to the surface was the control step of the reverse micelle extraction process. The extraction process can be explained by shrinking core model. The macroscopic dynamic equation of the protein forward extraction by the reverse micelles system was established and verified by extraction experiments at different temperatures. The experimental results indicated that the extraction process belonged to first order reaction and the apparent activation energy was 14.65kJ / mol. The kinetic equation model could better describe the reverse micelle extraction process of quinoa protein and could provide important theoretical reference for the comprehensive development and utilization of quinoa protein in the future.

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“…The results were shown in Figure 2 and 3. Results showed that in the extraction process, the reaction time was linearly related to 2 3…”

Section: Effect Of Temperature On Protein Extraction Ratementioning

confidence: 99%

“…Reverse micelle are agglomerates in which a surfactant is dissolved in a non-polar solvent [1,2]. In a reverse micelle solution, the hydrophobic groups of the surfactants come into contact with the solvent, and the hydrophilic groups face inward to form a polar core [3][4].…”

Section: Introductionmentioning

confidence: 99%

Study on Kinetics of Protein Forward Extraction from Quinoa by AOT/ Isooctane Reverse Micelles System

Wu¹,

Sun²,

Shi³

et al. 2018

Proceedings of the 2018 3rd International Conference on Modelling, Simulation and Applied Mathematics (MSAM 2018)

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“…Reverse micellar extraction (RME) has been recognized as an attractive alternative for downstream processing of biomolecules due to its simplicity, low surface tension at the interface and ease of scaling up [11]. Reverse micelles are optically transparent, self-assembled aggregates of surfactant clustered around water droplets suspended in an organic solvent [12]. The micelles are capable of solubilizing biomolecules of interest in its inner aqueous core according to their hydrophobicity, size, and charge without excessive loss of activities [13].…”

Section: ■ Introductionmentioning

confidence: 99%

Effect of Key Parameters on Jacalin Extraction from Aqueous Phase into Anionic Reverse Micelles

2019

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This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH 4 – 10), AOT concentration (5 – 150 mM), contact time (5 – 30 min) and phase volume ratio (0.5-5) on the transfer efficiency of jacalin was evaluated by changing one factor at a time (OFAT) while keeping the other parameters constant. A maximum of 83% of protein transfer was achieved after equal volume of organic and aqueous phase was stirred for 20 min using 20 mM AOT at pH 5 aqueous phase containing 0.1 M NaCl. The findings demonstrated AOT reverse micellar system as a promising and effective method to extract and purify jacalin from crude protein mixture.

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“…[44][45][46] In seminal studies by Ladanyi et al, it was shown that the RM water core dynamics is dependent on distance from the surfactant head molecule and water loading, [47][48][49][50] providing an explanation of water loading dependence of water dynamics within RMs. 51,52 Eskici and Axelsen recently estimated the size distribution of AOT RMs at a water loading of 7.5 via conventional molecular dynamics (MD) simulations 53 and investigated the water exchange mechanism upon the fusion of RMs. 54 Additionally, coarse-grained simulations of RM formation have demonstrated the role of the water core in the RM formation mechanism.…”

Section: Introductionmentioning

confidence: 99%

Characterization of dynamics and mechanism in the self-assembly of AOT reverse micelles

Urano

Pantelopulos

Song

et al. 2018

The Journal of Chemical Physics

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Reverse micelles (RMs) are recognized as a paradigm of molecular self-assembly and used in a variety of applications, such as chemical synthesis and molecular structure refinement. Nevertheless, many fundamental properties including their equilibrium size distribution, internal structure, and mechanism of self-assembly remain poorly understood. To provide an enhanced microscopic understanding of the assembly process and resulting structural distribution, we perform multiple nonequilibrium molecular dynamics simulations of dioctyl sulfosuccinate sodium salt (AOT) RM assembly, quantifying RM size, water core structure, and dynamics. Rapid assembly of smaller RM from a random mixture is observed to establish a constant AOT water loading within a nanosecond consistent with a diffusion-adsorption mechanism validated through the Monte-Carlo simulation of a model system. The structure of RM water cores and RM molecular volume during RM assembly is characterized during the AOT assembly process. A moment-closure equation is developed from a novel master equation model to elucidate the elementary events underlying the AOT selfassembly process. The resulting kinetic model is used to explore the role of monomer addition and dissociation, RM association and dissociation, and RM collision-induced exchange, all dependent on average RM size, which provides fundamental insight regarding the mechanisms and time scales for AOT RM self-assembly. The nascent dynamics that rapidly establish water loading, intermediate time scales of RM fusion, and longer time scale dynamics of inter-RM exchange essential in establishing the equilibrium condition are quantified through these kinetic models. Overall, this work provides insight into AOT RM self-assembly and provides a general theoretical framework for the analysis of the molecular self-assembly dynamics and mechanism.

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The Size of AOT Reverse Micelles

Cited by 51 publications

References 57 publications

Study on Kinetics of Protein Forward Extraction from Quinoa by AOT/ Isooctane Reverse Micelles System

Study on Kinetics of Protein Forward Extraction from Quinoa by AOT/ Isooctane Reverse Micelles System

Effect of Key Parameters on Jacalin Extraction from Aqueous Phase into Anionic Reverse Micelles

Characterization of dynamics and mechanism in the self-assembly of AOT reverse micelles

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