The size and conformation of <i>Artemia</i> (brine-shrimp) ribosomal RNA free in solution

Donceel, K.; Nieuwenhuysen, Paul; Clauwaert, J.

doi:10.1042/bj2050495

Cited by 3 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Smoluchowski encounter rate was calculated using Equation (2) (von Hippel and Berg 1989), (Donceel et al 1982;Nieuwenhuysen et al 1983) and 1.33 3 10 À6 cm 2 s À1 (Supplemental Fig. 4), respectively.…”

Section: Encounter Rate Calculationsmentioning

confidence: 99%

Evidence for the importance of electrostatics in the function of two distinct families of ribosome inactivating toxins

Korennykh¹,

Correll²,

Piccirilli³

2007

RNA

View full text Add to dashboard Cite

a-Sarcin and ricin represent two structurally and mechanistically distinct families of site-specific enzymes that block translation by irreversibly modifying the sarcin/ricin loop (SRL) of 23S-28S rRNA. a-Sarcin family enzymes are designated as ribotoxins and act as endonucleases. Ricin family enzymes are designated as ribosome inactivating proteins (RIP) and act as N-glycosidases. Recently, we demonstrated that basic surface residues of the ribotoxin restrictocin promote rapid and specific ribosome targeting by this endonuclease. Here, we report that three RIP: ricin A, saporin, and gypsophilin depurinate the ribosome with strong salt sensitivity and achieve unusually fast k cat /K m ;10 9 -10 10 M À1 s À1 , implying that RIP share with ribotoxins a common mechanism of electrostatically facilitated ribosome targeting. Bioinformatics analysis of RIP revealed that surface charge properties correlate with the presence of the transport chain in the RIP molecule, suggesting a second role for the surface charge in RIP transport. These findings put forward surface electrostatics as an important determinant of RIP activity.

show abstract

Section: Encounter Rate Calculationsmentioning

confidence: 99%

Evidence for the importance of electrostatics in the function of two distinct families of ribosome inactivating toxins

Korennykh¹,

Correll²,

Piccirilli³

2007

RNA

View full text Add to dashboard Cite

show abstract

“…The effects of a variety of cations on the equilibrium of eucaryotic wheat germ ribosomal subunits with 80S monosomes has been explored (Sperrazza et al, 1980(Sperrazza et al, , 1981Sperrazza & Spremulli, 1983; Moore & Spremulli, 1985), but no kinetic data were reported. Physicochemical studies have been carried out on Artemia (brine shrimp) ribosomes ; Donceel et al, 1982).…”

mentioning

confidence: 99%

Magnesium ion-dependent equilibria kinetics and thermodynamic parameters of Artemia ribosome dissociation and subunit association

Goss¹,

Harrigan²

1986

Biochemistry

View full text Add to dashboard Cite

The influence of magnesium ion concentration on the equilibrium and kinetics of Artemia ribosome dissociation and subunit association has been studied by laser light scattering. Ribosomal aggregation was found to be reduced by addition of 0.1-0.05 mM spermidine and KCl concentrations of 100 mM. The ribosomes were found to be stable at low [Mg2+], and the curves obtained for ribosome-subunit equilibrium were independent of the direction and origin of the magnesium ion titration. Thermodynamic parameters were obtained from the temperature-dependent equilibria and have been compared to those of wheat germ and Escherichia coli type A ribosomes. The entropy term calculated for the association of 40S and 60S subunits is small, and the reaction is exothermic. The entropy term is negative, favoring subunit dissociation, and contributes less to the free energy than the enthalpy term. Rate constants for ribosome dissociation and subunit association have been determined. The reaction curves gave no evidence for sequential processes and were homogeneous.

show abstract

Shape and compactness of the isolated ribosomal 16 S RNA and its complexes with ribosomal proteins

Serdyuk

Agalarov

Sedelnikova

et al. 1983

Journal of Molecular Biology

View full text Add to dashboard Cite

The size and conformation of Artemia (brine-shrimp) ribosomal RNA free in solution

Cited by 3 publications

References 26 publications

Evidence for the importance of electrostatics in the function of two distinct families of ribosome inactivating toxins

Evidence for the importance of electrostatics in the function of two distinct families of ribosome inactivating toxins

Magnesium ion-dependent equilibria kinetics and thermodynamic parameters of Artemia ribosome dissociation and subunit association

Shape and compactness of the isolated ribosomal 16 S RNA and its complexes with ribosomal proteins

Contact Info

Product

Resources

About