The Six-Point Design in the U. S. P. Microbiological Assay of Vitamin B12

Campbell, J. A.; McLaughlan, J. M.; Clark, Jean A.; Dunnett, Charles W.

doi:10.1002/jps.3030420503

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1953

1973

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Cited by 6 publications

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References 20 publications

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“…The L. leuhmannii method was the U. S. P. procedure (1) modified by the use of the 6 point design (3). Samples were extracted with a buffered 1% solution of sodium mela-bisulfite (2).…”

Section: General Proceduresmentioning

confidence: 99%

“…It is the official method in the United States (1,2) and is also used in Canada for the assay of vitamin BIZ in pharmaceuticals. Along with other workers, the authors (3) have found that it yields precise and reproducible results but suffers from the disadvantage that the organism is extremely sensitive to the way it is grown and prepared for assay. It has also been shown (4, 5) that glassware, for use in this assay, must be cleaned with considerable care.…”

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confidence: 99%

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The influence of thiamine on the microbiological assay for vitamin B12 with Escherichia coli 113‐3

McLaughlan¹,

Rogers²,

Campbell³

1955

Journal of the American Pharmaceutical Association (Scientific

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“…The L. leuhmannii method was the U. S. P. procedure (1) modified by the use of the 6 point design (3). Samples were extracted with a buffered 1% solution of sodium mela-bisulfite (2).…”

Section: General Proceduresmentioning

confidence: 99%

mentioning

confidence: 99%