The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula

Pereira, Wendell J.; Boyd, Jade; Conde, Daniel; Triozzi, Paolo M.; Balmant, Kelly M.; Dervinis, Christopher; Schmidt, Henry W.; Boaventura-Novaes, Carolina; Chakraborty, Sanhita; Knaack, Sara A.; Gao, Yueyao; Feltus, Frank Alexander; Roy, Sushmita; Ané, Jean-Michel; Frugoli, Julia; Kirst, Matias

doi:10.1016/j.celrep.2024.113747

Cited by 6 publications

(1 citation statement)

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“…P. betavasculorum strains possess isopentenyl transferase (IPT) enzyme gene in their genomes and several other genes with IPT activity, such as tRNA-IPTs and adenylate IPTs, which can produce various forms of cytokines, including the most active form, trans-Zeatin. Cytokines produced by bacteria may promote plant growth and enhance their resistance under stress conditions ( Wei et al., 2023 ; Pereira et al., 2024 ). However, some phytopathogenic bacteria increase cytokine production at the infection site, which lengthens their survival in plant tissues ( Wei et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics, pangenomics, and phenomic studies of Pectobacterium betavasculorum strains isolated from sugar beet, potato, sunflower, and artichoke: insights into pathogenicity, virulence determinants, and adaptation to the host plant

Borowska-Beszta,

Smoktunowicz,

Horoszkiewicz

et al. 2024

Front. Plant Sci.

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IntroductionBacteria of genus Pectobacterium, encompassing economically significant pathogens affecting various plants, includes the species P. betavasculorum, initially associated with beetroot infection. However, its host range is much broader. It causes diseases of sunflower, potato, tomato, carrots, sweet potato, radish, squash, cucumber, and chrysanthemum. To explain this phenomenon, a comprehensive pathogenomic and phenomic characterisation of P. betavasculorum species was performed.MethodsGenomes of P. betavasculorum strains isolated from potato, sunflower, and artichoke were sequenced and compared with those from sugar beet isolates. Metabolic profiling and pathogenomic analyses were conducted to assess virulence determinants and adaptation potential. Pathogenicity assays were performed on potato tubers and chicory leaves to confirm in silico predictions of disease symptoms. Phenotypic assays were also conducted to assess the strains ability to synthesise homoserine lactones and siderophores.ResultsThe genome size ranged from 4.675 to 4.931 kbp, and GC % was between 51.0% and 51.2%. The pangenome of P. betavasculorum is open and comprises, on average, 4,220 gene families. Of these, 83% of genes are the core genome, and 2% of the entire pangenome are unique genes. Strains isolated from sugar beet have a smaller pangenome size and a higher number of unique genes than those from other plants. Interestingly, genomes of strains from artichoke and sunflower share 391 common CDS that are not present in the genomes of other strains from sugar beet or potato. Those strains have only one unique gene. All strains could use numerous sugars as building materials and energy sources and possessed a high repertoire of virulence determinants in the genomes. P. betavasculorum strains were able to cause disease symptoms on potato tubers and chicory leaves. They were also able to synthesise homoserine lactones and siderophores.DiscussionThe findings underscore the adaptability of P. betavasculorum to diverse hosts and environments. Strains adapted to plants with high sugar content in tissues have a different composition of fatty acids in membranes and a different mechanism of replenishing nitrogen in case of deficiency of this compound than strains derived from other plant species. Extensive phenomics and genomic analyses performed in this study have shown that P. betavasculorum species is an agronomically relevant pathogen.

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Section: Discussionmentioning

confidence: 99%