The Single Antigen expressing Lines (SALs) Concept: An Excellent Tool for Screening for HLA-Specific Antibodies

Human leukocyte antigen antibody detection and kidney allocation within Eurotransplant

“…This was confirmed by a study of Van den BergLoonen et al [11], who showed, in a retrospective study, that in patients undergoing transplantation in the acceptable mismatch program on basis of a negative CDC crossmatch, the presence of donor-specific antibodies detectable in Luminex was not associated with a poorer graft survival compared with patients without donor specific antibodies in Luminex. The presence of donor specific antibodies in Luminex was associated with a higher incidence of acute rejection in the first 6 months but this did not affect graft survival at the long term.…”

Mature wines are better: CDC as the leading method to define highly sensitized patients

“…In essence these solid SPAs are based on the solubilization of the molecules from the cell surface of either EpsteinBarr virus (EBV) cell lines or lines expressing a single HLA molecule after transfection with the respective sequence [9]. The molecules are bound on the surface of the assay in question.…”

Allo-HLA reactivity of virus-specific memory T cells is common

“…K562 and EBV-LCLs expressing single allo-HLA molecules were generated by transduction with retroviral vectors encoding for the allo-HLA molecules or by transfection of allo-HLA molecules. 27,28 For the isolation of T cells, B cells, and monocytes, peripheral blood mononuclear cells of healthy donors were stained with either anti-CD3, anti-CD19, or anti-CD14 magnetic-activated cell sorting beads (Miltenyi Biotec), respectively, and isolated according to the manufacturer's instructions. CD40 ligand (CD40L)-activated B cells were generated by culturing the CD19 ϩ fraction for 3 days on CD40L-transduced murine fibroblasts 29 in medium containing CpG (10 g/mL) and IL-4 (500 IU/mL; Schering-Plough).…”