The simultaneous quantification of phytosterols and tocopherols in liposomal formulations using validated atmospheric pressure chemical ionization- liquid chromatography –tandem mass spectrometry

Poudel, Asmita; Gachumi, George; Badea, Ildikó; Bashi, Zafer Dallal; El‐Aneed, Anas

doi:10.1016/j.jpba.2020.113104

Cited by 13 publications

(10 citation statements)

References 31 publications

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“…The results obtained showed that the developed and validated method is cheaper and simpler than the other methods in the literature such as chromatographic, voltammetric and spectroscopic methods (20,24,25). And also these results showed that the developed method presice and accurate for the quantitaive determination.…”

Section: Discussionmentioning

confidence: 69%

“…Precision and accuracy of the proposed method was determined by analysing the quality control samples in the same day and on three diffrent days at three different concentration (25,50, 75 μg/mL) (n=6).…”

Section: Precision and Accuracymentioning

confidence: 99%

“…For the determination of α-tocopherol, several methods have been reported in different samples such as foods (18), cosmetics (19), biological fluids (20)(21)(22), natural compounds (23) and pharmaceutical preparations (24) in the literature. Chromatographic methods such as gas chromatography, liquid chromatography and spectroscophic methods such as mass spectrometry, UV-Vis spectrophotometry, flourescence spectrophotometry have been widely used for the quantification of tocopherols (25). Although mostly chromatographic methods such as high performance liquid chromatography (HPLC) are used, spectrophotometric methods are also highly preferred due to their simplicity and specifity for determination of pharmaceutical preparations (24).…”

Section: Introductionmentioning

confidence: 99%

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Farmasötik Yumuşak Kapsülde α-Tokoferolün Spektrofotometri ile Kantitatif Tayini

Artuç¹

2020

Experimed

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Objective: Vitamin E is an essential micronutrient for maintaining a healthy status and preventing disease. The purpose of this study was to develop and validate a simple, sensitive and easily applicable spectrophotometric method for determination of α-tocopherol in pharmaceutical preparations. Material and Method: The quantitative determination of the α-tocopherol in pharmaceutical prepation was carried out using the maximum absorbance value measured at 290 nm. Calibration graphs were constructed by plotting the absorbance against the corresponding concentration of standart α-tocopherol samples at five different concentrations (10-100 µg/mL). Results: The amount of α-tocopherol in the pharmaceutical soft capsule was calculated as 101.572% (203.145 IU/capsule) (Evicap soft capsule labelled content: 200 IU/capsule). Conclusion: It suggested that the developed spectrophotometric method in this study is accurate, sensitive, precise, reproducible and easily applied to soft capsules and the other pharmaceutical preparations.

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Section: Discussionmentioning

confidence: 69%

Section: Precision and Accuracymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Farmasötik Yumuşak Kapsülde α-Tokoferolün Spektrofotometri ile Kantitatif Tayini

Artuç¹

2020

Experimed

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“…A reversed-phase LC-MS method was developed using ESI for the characterization of a variety of synthetic stigmasterol oxidation products, and nuclear magnetic resonance (NMR) was also used to complement MS results [70]. However, ESI is not ideal for ionizing hydrophobic compounds as it shows lower ionization efficiency [71].…”

Section: Liquid Chromatographymentioning

confidence: 99%

Analytical Strategies to Analyze the Oxidation Products of Phytosterols, and Formulation-Based Approaches to Reduce Their Generation

2021

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Phytosterols are a class of lipid molecules present in plants that are structurally similar to cholesterol and have been widely utilized as cholesterol-lowering agents. However, the susceptibility of phytosterols to oxidation has led to concerns regarding their safety and tolerability. Phytosterol oxidation products (POPs) present in a variety of enriched and non-enriched foods can show pro-atherogenic and pro-inflammatory properties. Therefore, it is crucial to screen and analyze various phytosterol-containing products for the presence of POPs and ultimately design or modify phytosterols in such a way that prevents the generation of POPs and yet maintains their pharmacological activity. The main approaches for the analysis of POPs include the use of mass spectrometry (MS) linked to a suitable separation technique, notably gas chromatography (GC). However, liquid chromatography (LC)-MS has the potential to simplify the analysis due to the elimination of any derivatization step, usually required for GC-MS. To reduce the transformation of phytosterols to their oxidized counterparts, formulation strategies can theoretically be adopted, including the use of microemulsions, microcapsules, micelles, nanoparticles, and liposomes. In addition, co-formulation with antioxidants, such as tocopherols, may prove useful in substantially preventing POP generation. The main objectives of this review article are to evaluate the various analytical strategies that have been adopted for analyzing them. In addition, formulation approaches that can prevent the generation of these oxidation products are proposed.

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“…Typically, this is a guard column, and it can be argued that the use of a guard column constitutes a modified version of FIA-MS; the terminology is more commonly referred to as “fast chromatography”. , Using a guard column provides a compromise between combining the simplicity and high throughput of FIA-MS with the capability to distinguish interferences with chromatography. Recently the use of a FIA-MS/MS method for the simultaneous determination of tocopherols and plant sterols was implemented to simplify an LC-MS/MS approach. , However, interferences were observed among the plant sterols during FIA-MS/MS. For example, β-sitosterol ionizes as [M + H – H 2 O] + at m / z 397 but a similar ion is also formed from the in-source ionization of campesterol [M + H – 4H] + .…”

Section: Flow Injection Analysis-mass Spectrometry (Fia-ms)mentioning

confidence: 99%

Fast Quantification Without Conventional Chromatography, The Growing Power of Mass Spectrometry

et al. 2020

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Mass spectrometry (MS) in hyphenated techniques is widely accepted as the gold standard quantitative tool in life sciences. However, MS possesses intrinsic analytical capabilities that allow it to be a stand-alone quantitative technique, particularly with current technological advancements. MS has a great potential for simplifying quantitative analysis without the need for tedious chromatographic separation. Its selectivity relies on multistage MS analysis (MSn), including tandem mass spectrometry (MS/MS), as well as the ever-growing advancements of high-resolution MS instruments. This perspective describes various analytical platforms that utilize MS as a stand-alone quantitative technique, namely, flow injection analysis (FIA), matrix assisted laser desorption ionization (MALDI), including MALDI-MS imaging and ion mobility, particularly high-field asymmetric waveform ion mobility spectrometry (FAIMS). When MS alone is not capable of providing reliable quantitative data, instead of conventional liquid chromatography (LC)-MS, the use of a guard column (i.e., fast chromatography) may be sufficient for quantification. Although the omission of chromatographic separation simplifies the analytical process, extra procedures may be needed during sample preparation and clean-up to address the issue of matrix effects. The discussion of this manuscript focuses on key parameters underlying the uniqueness of each technique for its application in quantitative analysis without the need for a chromatographic separation. In addition, the potential for each analytical strategy and its challenges are discussed as well as improvements needed to render them as mainstream quantitative analytical tools. Overcoming the hurdles for fully validating a quantitative method will allow MS alone to eventually become an indispensable quantitative tool for clinical and toxicological studies.

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The simultaneous quantification of phytosterols and tocopherols in liposomal formulations using validated atmospheric pressure chemical ionization- liquid chromatography –tandem mass spectrometry

Cited by 13 publications

References 31 publications

Farmasötik Yumuşak Kapsülde α-Tokoferolün Spektrofotometri ile Kantitatif Tayini

Farmasötik Yumuşak Kapsülde α-Tokoferolün Spektrofotometri ile Kantitatif Tayini

Analytical Strategies to Analyze the Oxidation Products of Phytosterols, and Formulation-Based Approaches to Reduce Their Generation

Fast Quantification Without Conventional Chromatography, The Growing Power of Mass Spectrometry

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