The Similarity of Effects of Vasopressin, Adenosine-3′,5′-Phosphate (Cyclic Amp) and Theophylline on the Toad Bladder*

Orloff, Jack; Handler, Jerome S.

doi:10.1172/jci104528

Cited by 476 publications

(157 citation statements)

References 16 publications

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“…The sensitivity of isolated bladder lobes to vasopressin The effect of 3',5'-adenosine monophosphate in the presence of pentobarbitone, thiopentone or chloralose Orloff & Handler (1962) showed that 3',5'-adenosine monophosphate (cyclic AMP) introduced into the medium surrounding the isolated toad bladder renders the wall permeable to water. The effect of anaesthetics on this action was investigated in the same manner as their effect on the action of vasopressin.…”

Section: Resultsmentioning

confidence: 99%

The Influence of Anaesthetics on the Increase in the Water Permeability of the Toad Bladder Induced by Vasopressin

Grey

Ullmann

1974

British J Pharmacology

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1Single lobes of the bladder of Bufo marinus were isolated and filled with, and suspended in, oxygenated Ringer solution. The fluid in contact with the outside (serosa) of the lobes had a total osmolarity of 225 m-osmol/litre, and that bathing the inside (mucosa) of 45 m-osmol/ litre. 2 Osmotic water flow from mucosa to serosa was measured by weighing the lobes every 30 minutes. It was negligible unless vasopressin was added to the serosal bath. Standard concentrations of either 1.25 or 6.25 mu/ml were used to render the bladder lobes permeable to water.3 The presence in the serosal medium of pentobarbitone or thiopentone in concentrations ranging from 0.25 to 2.5 mM, or of chloralose in concentrations ranging from 0.65 to 6.5 mM, diminished the increase in water permeability induced by vasopressin. 4 The three anaesthetics exerted similar inhibitory effects on the action of vasopressin from the serosal and from the mucosal surface of the bladder. 5 In the presence of a constant high concentration of anaesthetic, increasing the concentration of vasopressin over three orders of magnitude led to stepwise increases of osmotic water flow out of the lobes, although at every dose level the effect of vasopressin was depressed by the anaesthetic. However, it was not completely abolished even if the concentration of vasopressin was close to threshold. 6 The increase in water permeability of the bladder induced by 3',5'-adenosine monophosphate (cyclic AMP) was also depressed by the three anaesthetics. 7 Possible explanations of the findings are discussed.

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Section: Resultsmentioning

confidence: 99%

The Influence of Anaesthetics on the Increase in the Water Permeability of the Toad Bladder Induced by Vasopressin

Grey

Ullmann

1974

British J Pharmacology

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“…A great deal of evidence indicates that cyclic AMP mediates the action of antidiuretic hormone on renal tubules and other epithelial membranes (10)(11)(12)(13). In vitro studies have shown that PGE, impairs the ability of vasopressin to stimulate cyclic AMP production (2).…”

Section: Discussionmentioning

confidence: 99%

In vivo effect of indomethacin to potentiate the renal medullary cyclic AMP response to vasopressin.

Lum¹,

Aisenbrey²,

Dünn³

et al. 1977

J. Clin. Invest.

118

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“…It could, as previously suggested (18) initiate directly a structural alteration in the mucosal membrane of the bladder by triggering a wave of disulfidesulfhydryl interchanges. However, if the vasopressin-like actions of cyclic AMP and theophylline (20) on bladder permeability are not adventitious pharmacologic phenomena, this simplest of models is no longer tenable. In any case, it seems likely that there are multiple events between hormonereceptor interaction and the final change in permeability.…”

Section: Changes In Positionmentioning

confidence: 99%

Structural Requirements for the Action of Neurohypophyseal Hormones upon the Isolated Amphibian Urinary Bladder

Rasmussen

Schwartz

Young

et al. 1963

The Journal of General Physiology

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The response of the isolated amphibian urinary bladder to thirtyfour structural analogs of arginine vasotocin was determined in an effort to define the physiological significance of specific structural groups on the hormone molecule. All but one of the analogs tested possessed full intrinsic activity in this system but varied greatly in their affinity for the receptor site. An analysis of the effect of changes in hydrogen ion concentration upon the response of the bladder to oxytocin was performed in order to determine the number and nature of the ionizable groups involved in hormone receptor interaction. Two ionizable groups with apparent pK's of 7.1 and 7.75 were found to be important in determining the magnitude of the hormonal response. On the basis of the results it was postulated that hormone-receptor interaction can be considered a two-step process: (a) The binding or attachment of hormone to receptor site through ionic, hydrogen, and hydrophobic bonds and (b) a disulfide interchange reaction between hormonal disulfide and receptor sulfhydryl. The latter step is considered to be the reaction which initiates the chain of events leading to the observed change in permeability.Although the distinction between the affinity and intrinsic activity of a drug for its receptor site has received theoretical consideration and application in pharmacology (1), little usefulness has been found for these concepts in the study of hormone-receptor interactions. However, because of the recent developments in the isolation, characterization, and synthesis of polypeptide hormones, they m a y become of practical value in the study of hormonereceptor interactions. The classic work of du Vigneaud and his colleagues on the neurohypophyseal hormone (2-7) has evoked new and exciting possibilities in the study of the correlation between structure and activity of II7I

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The Similarity of Effects of Vasopressin, Adenosine-3′,5′-Phosphate (Cyclic Amp) and Theophylline on the Toad Bladder*

Cited by 476 publications

References 16 publications

The Influence of Anaesthetics on the Increase in the Water Permeability of the Toad Bladder Induced by Vasopressin

The Influence of Anaesthetics on the Increase in the Water Permeability of the Toad Bladder Induced by Vasopressin

In vivo effect of indomethacin to potentiate the renal medullary cyclic AMP response to vasopressin.

Structural Requirements for the Action of Neurohypophyseal Hormones upon the Isolated Amphibian Urinary Bladder

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