The SikCuZnSOD3 gene improves abiotic stress resistance in transgenic cotton

Zhang, Li; Tian, Wenhui; Huang, Gang; Liu, Bucang; Wang, Aiying; Zhu, Jianbo; Guo, Xianguang

doi:10.1007/s11032-021-01217-0

Cited by 8 publications

(2 citation statements)

References 55 publications

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“…Under cold stress, trehalose application modulated the expression profile of the Cu/ZnSOD gene in tomato plants [ 16 ]. In a recent study, the overexpression of SikCuZnSOD3 enhances tolerance to cold, drought, and salinity stresses in cotton ( Gossypium hirsutum ) [ 18 ]. Under water deficit conditions, wheat ( Triticum aestivum ) plants showed a higher expression of different antioxidant encoding genes, including MnSOD [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis and Expression Profile of Superoxide Dismutase (SOD) Gene Family in Rapeseed (Brassica napus L.) under Different Hormones and Abiotic Stress Conditions

Raza

Gao

et al. 2021

Antioxidants

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Superoxide dismutase (SOD) is an important enzyme that acts as the first line of protection in the plant antioxidant defense system, involved in eliminating reactive oxygen species (ROS) under harsh environmental conditions. Nevertheless, the SOD gene family was yet to be reported in rapeseed (Brassica napus L.). Thus, a genome-wide investigation was carried out to identify the rapeseed SOD genes. The present study recognized 31 BnSOD genes in the rapeseed genome, including 14 BnCSDs, 11 BnFSDs, and six BnMSDs. Phylogenetic analysis revealed that SOD genes from rapeseed and other closely related plant species were clustered into three groups based on the binding domain with high bootstrap values. The systemic analysis exposed that BnSODs experienced segmental duplications. Gene structure and motif analysis specified that most of the BnSOD genes displayed a relatively well-maintained exon–intron and motif configuration within the same group. Moreover, we identified five hormones and four stress- and several light-responsive cis-elements in the promoters of BnSODs. Thirty putative bna-miRNAs from seven families were also predicted, targeting 13 BnSODs. Gene ontology annotation outcomes confirm the BnSODs role under different stress stimuli, cellular oxidant detoxification processes, metal ion binding activities, SOD activity, and different cellular components. Twelve BnSOD genes exhibited higher expression profiles in numerous developmental tissues, i.e., root, leaf, stem, and silique. The qRT-PCR based expression profiling showed that eight genes (BnCSD1, BnCSD3, BnCSD14, BnFSD4, BnFSD5, BnFSD6, BnMSD2, and BnMSD10) were significantly up-regulated under different hormones (ABA, GA, IAA, and KT) and abiotic stress (salinity, cold, waterlogging, and drought) treatments. The predicted 3D structures discovered comparable conserved BnSOD protein structures. In short, our findings deliver a foundation for additional functional investigations on the BnSOD genes in rapeseed breeding programs.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis and Expression Profile of Superoxide Dismutase (SOD) Gene Family in Rapeseed (Brassica napus L.) under Different Hormones and Abiotic Stress Conditions

Raza

Gao

et al. 2021

Antioxidants

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“…During periods of drought, the expression of the MnSOD gene in wheat (Triticum aestivum) was significantly increased [17]. It is worth mentioning that according to reports, the overexpression of SikCuZnSOD3 has been found to enhance the resistance of cotton (Gossypium hirsutum) to drought, salt, and cold stresses [18]. In rice (Oryza sativa), transgenic plants' overexpression of OsMSD1 displayed higher tolerance to heat, whereas knock-out msd1 mutants was notably susceptible to the same stress [19].…”

Section: Introductionmentioning

confidence: 99%

Identification and Analysis of SOD Family Genes in Peanut (Arachis hypogaea L.) and Their Potential Roles in Stress Responses

Wang

Wang³

et al. 2023

Agronomy

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Superoxide dismutases (SODs) are crucial in safeguarding plants against reactive oxygen species (ROS) toxicity caused by abiotic or biotic factors. Although recent research has revealed the involvement of the SOD gene family in plant biological processes, the understanding of the SOD gene family in peanut remains inadequate. This study comprehensively characterizes the SOD gene family in the peanut genome. A total of 25 AhSOD genes were identified and subsequently categorized into three subfamilies: sixteen AhCSDs, six AhFSDs, and three AhMSDs according to the phylogenetic tree. A comprehensive analysis revealed that the AhSOD genes underwent segmental duplications. The majority of AhSOD genes exhibited conserved exon–intron and motif structures within the same subfamily. The examination of cis-acting elements within the promoter regions of SOD genes revealed that the expression of AhSOD was subject to regulation by plant hormones, as well as responses to defense and stress. RNA-seq analysis showed expression diversity of AhSOD genes in various tissues and cold, drought, and salt stresses. Furthermore, the regulation of AhSOD gene expression is anticipated to involve numerous transcription factors. The gene ontology annotation results validate the role of AhSOD genes in various stress stimuli, SOD activity, reactive oxygen species metabolic processes, and cellular oxidant detoxification processes. This investigation serves as the initial genome-wide analysis of the AhSOD gene family, providing a basis for comprehending the function of the AhSOD gene family and enhancing plant tolerance to cold, drought, and salt stresses.

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