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The study determined the effects of partial removal of alkaloids (detoxification) in crushed lupin seed (CLS) on voluntary food intake, and yield and composition of milk from dairy cattle. Twenty multiparous Friesian dairy cows (first 90 days of lactation) were assigned, according to a randomized complete-block design, to five diets. The diets were formulated to be isonitrogenous (25·6 g N per kg of diet dry matter (DM)) and contained napier grass, lucerne hay, maize bran and urea. The control diet (CON) contained sunflower meal; two diets contained intact CLS at 150 (LUI-15) or 300 g (LUl-30) per kg diet DM. The other two diets contained detoxified CLS at 150 (LUD-15) or 300 g (LUD-30) per kg diet DM. Lupin seeds were detoxified by treatment with boiling water, followed by steeping in cold water. The diets were analysed by gas chromatography for the alkaloids, lupanine and 13-hydroxylupanine. The total alkaloid content of LUI-15 and LUl-30 was 3·8 and 8·0 g/kg diet DM, respectively; by contrast that of LUD-15 and LUD-30 was 2·1 and 5·2 g/kg diet DM respectively. Increasing the level of intact CLS in the diet led to a decrease in voluntary food intake. Cows given LUl-30 had a lower milk yield (11·1 kg/day) than those given LUI-15 (13·8 kg/day;P< 0·01) but there was no difference in either milk protein yield or content. Detoxification of lupin removed proportionately about 0·40 of the total alkaloids from intact CLS and increased food intake, and the yield of milk and milk protein (P< 0·05) but reduced milk fat content. Detoxification of CLS also reduced the rumen degradability of lupin protein (P< 0·05). It is concluded that the reduction in organic matter intake and milk yield of cows given diets containing intact CLS was due to the presence of lupanine and 13-hydroxylupanine. To maximize its usage in diets for dairy cattle, lupin should be detoxified; it can then be included at levels up to 300 g/kg diet DM.
The study determined the effects of partial removal of alkaloids (detoxification) in crushed lupin seed (CLS) on voluntary food intake, and yield and composition of milk from dairy cattle. Twenty multiparous Friesian dairy cows (first 90 days of lactation) were assigned, according to a randomized complete-block design, to five diets. The diets were formulated to be isonitrogenous (25·6 g N per kg of diet dry matter (DM)) and contained napier grass, lucerne hay, maize bran and urea. The control diet (CON) contained sunflower meal; two diets contained intact CLS at 150 (LUI-15) or 300 g (LUl-30) per kg diet DM. The other two diets contained detoxified CLS at 150 (LUD-15) or 300 g (LUD-30) per kg diet DM. Lupin seeds were detoxified by treatment with boiling water, followed by steeping in cold water. The diets were analysed by gas chromatography for the alkaloids, lupanine and 13-hydroxylupanine. The total alkaloid content of LUI-15 and LUl-30 was 3·8 and 8·0 g/kg diet DM, respectively; by contrast that of LUD-15 and LUD-30 was 2·1 and 5·2 g/kg diet DM respectively. Increasing the level of intact CLS in the diet led to a decrease in voluntary food intake. Cows given LUl-30 had a lower milk yield (11·1 kg/day) than those given LUI-15 (13·8 kg/day;P< 0·01) but there was no difference in either milk protein yield or content. Detoxification of lupin removed proportionately about 0·40 of the total alkaloids from intact CLS and increased food intake, and the yield of milk and milk protein (P< 0·05) but reduced milk fat content. Detoxification of CLS also reduced the rumen degradability of lupin protein (P< 0·05). It is concluded that the reduction in organic matter intake and milk yield of cows given diets containing intact CLS was due to the presence of lupanine and 13-hydroxylupanine. To maximize its usage in diets for dairy cattle, lupin should be detoxified; it can then be included at levels up to 300 g/kg diet DM.
The effects of pasture quality and the amount and nutrient composition of a concentrate supplement on performance of lactating cows were investigated under a controlled rotational grazing system. Pasture samples were collected for two consecutive summers, and the relationship was positive between wet chemistry and near infrared reflectance spectroscopy for CP, ADF, and NDF. During yr 2, 54 Holstein cows averaging 82 d postpartum were used in a randomized block design to evaluate three methods of supplementing grain for a 24-wk trial. A 16.2-ha area of primarily orchardgrass pasture was divided into paddocks of approximately .40 ha each. The three supplemental grain rations were: 1) 1 kg of grain/3 kg of milk; 2) 1 kg of grain/4 to 5 kg of milk adjusted weekly, depending upon the quantity of pasture available; and 3) 1 kg of grain/4 to 5 kg of milk. The amount fed in ration 3 was adjusted weekly, depending upon the quantity of pasture available, and reformulated biweekly based on analysis of pasture. No differences existed among treatments for milk production (24.7, 23.2, and 24.1 kg/d) or 3.5% FCM (25.3, 24.8, and 24.9 kg/d) over the 24-wk trial. Cows fed more grain (ration 1) gained more BW. Adjusting nutrient density of the grain to changes in pasture quality resulted in similar milk production and a lower ration cost.
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