The significance of intracytoplasmic proteins in Reed-Sternberg cells

Abstract

Lymphoid tissue of 42 patients with Hodgkin's disease was studied with immunohistological techniques on the light microscopic and ultrastructural level. The presence of IgG in some Reed‐Sternberg (R‐S) cells was confirmed, and in addition serial sections and a double staining technique revealed that these cells also contained both kappa and lambda light chains. Furthermore two serum proteins, human serum albumin and alpha‐1‐antitrypsin, were demonstrated in the same positive R‐S cells. The ultrastructural localization of the immunoglobulin and of human serum albumin was not related to any protein synthesizing organelle or to structures related to endocytosis. It is suggested that the presence of immunoglobulin in R‐S cells is the result of a disturbance of the cell wall integrity with subsequent nonspecific diffusion of immunoglobulin and other serum proteins into the cell. The presence of IgG therefore can not be taken as an argument for a B‐cell origin of R‐S cells. Possible mechanisms for the cell wall damage are discussed. Cancer 42:1793–1803, 1978.

Macrophage origin of Reed-Sternberg cells: an immunohistochemical study

“…Other immunoglobulins, albumin, a1AT and oflACT, have also been found within RS cells'0 [15][16][17] but reports of their incidence vary widely and little attention has been paid to their staining pattern or cytoplasmic distribution. In this study we have shown that II serum proteins other than IgG can be demonstrated in RS cells but that, contrary to other reports,17 their incidence and staining pattern differ from IgG and they are thus thought to represent a different phenomenon.…”

“…This distribution has been interpreted as indicating both specific ribosomal synthesis18 19 and nonspecific leakage into the cell. '7 Other serum proteins have been found in RS cells but less commonly than IgG [15][16][17] In the light of the conflicting interpretations as to the extent and significance of IgG and other proteins in RS cells we have performed an immunoperoxidase study of 12 different serum proteins including IgG, on a series of biopsies from patients with Hodgkin's disease. We have stained for J chain which although associated extracellularly only with dimeric IgA and 19 S IgM is synthesised in cells producing immunoglobulin of all classes.…”

Double-labeling for Keratin and Class III β-Tubulin Within Cultured Retinal Pigment Epithelial Cells: Comparison of Chromogens to Yield Maximum Resolution of Two Structural Proteins Within The Same Cell

“…Warm slides to RT (1 hr) if incubated at 4OC. 6. Wash for 10 min with 1% NGS (for polyclonal antibodies) or 1% NRS (for monoclonal antibodies) in TBS.…”

“…This technique is widely used for visualizing different cell populations in which neither population contains both antigens (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Most examples of two distinct antigens demonstrating within the same cell have utilized immunofluorescence with multiple fluorescent markers or immunofluorescence in combination with immunoenzymatic labeling (17,(26)(27)(28)(29)(30).…”

Walter Hubert Lecture, delivered 1 April 1980 to the 21st AGM of the BACR Malignant lymphomas as tumours of the immune system

“…The finding of immunoglobulins, both within (Garvin et at., 1974: Taylor, 1974, 1976Payne et al, 1976) and on the surface (Kadin et al, 1974;Boecker et al, 1975) (Garvin et al, 1974;Taylor, 1974Taylor, , 1976Kadin et al, 1978;Poppema et al, 1978). Certainly the mere presence of immunoglobulin, without evidence of endogenous synthesis, does not identify a cell as a B lymphocyte.…”