The significance of elevated plasma expression of microRNA 106b~25 clusters in gastric cancer

Abstract

ObjectiveConcentrating on oncogenic role and increased plasma expression of microRNA(miR) 106b~25 clusters (involving miR 106b, miR 93 and miR 25), we evaluated significance of the over-expression of plasma miR 106b~25 in GC.MethodsBased on 65 pairs matched GC patients and health controls, we explored clinical significance of miR 106b~25 for GC and compared their diagnostic performance with conventional tumor biomarkers including CA724, CA242, CA199 and CEA.ResultsBoth miR 106b~25 cluster and conventional tumor biomarkers were significantly elevated in GC (All P<0.05). In ROC curves, miR 106b had the highest AUC (0.898) in diagnosing GC with optimal sensitivity of 86.2% and specificity of 92.3% at the cut-off value of 1.385. MiR 25 had moderate diagnostic efficacy (AUC = 0.817) with sensitivity of 87.6% and specificity of 76.9% at the threshold of 1.015. The AUC of miR 93 (0.756) was the lowest. The AUC, sensitivity, accuracy and Youden index of miR 106b were higher than all of four conventional biomarkers, while its specificity is higher than CA242 and CA724. The AUC of miR 25 was also higher than CA724, CA242 and CA199, while AUC of miR 93 was only higher than CA199 and CA724. Compared the diagnostic efficacy via ROC curves, miR 106b was significantly higher diagnostic efficacy than CA724, CA242 and CA199, the diagnostic efficacies of miR 93 and miR 25 were significantly higher than CA199(all P<0.05).ConclusionsPlasma miR 106b~25 cluster, especially miR 106b, were significantly increased in GC patients and may be hopeful diagnostic biomarkers.

MicroRNA‑495 suppresses cell proliferation and invasion of hepatocellular carcinoma by directly targeting insulin‑like growth factor receptor‑1

“…miRNAs negatively regulate gene expression by typically binding to complementary sequences in 3'-untranslated regions (3'-UTRs) of their target genes and therefore stimulate mRNA degradation or translational inhibition (10). Altered miRNA expression has been described in various types of human malignancies, such as HCC (11), renal cell carcinoma (12), gastric cancer (13) and colorectal cancer (14). miRNA dysregulation has been demonstrated to be involved in various cellular biological processes, including cell proliferation, cell cycle, apoptosis, angiogenesis, invasion, migration, epithelial-to-mesenchymal transition and metastasis (15)(16)(17).…”

“…miRNA dysregulation has been demonstrated to be involved in various cellular biological processes, including cell proliferation, cell cycle, apoptosis, angiogenesis, invasion, migration, epithelial-to-mesenchymal transition and metastasis (15)(16)(17). In human cancer, miRNAs may serve as tumour suppressors or oncogenes which mainly depend on the biological roles of their target genes (13). Highly expressed miRNAs act as oncogenes by inhibiting tumour suppressor gene function, whereas downregulated miRNAs function as tumour suppressor genes through oncogene downregulation (14,15).…”

Molecular mechanism and role of microRNA‐93 in human cancers: A study based on bioinformatics analysis, meta‐analysis, and quantitative polymerase chain reaction validation

“…Separately, 11 studies from eight articles 15,[56][57][58][59][60][61][62] were included in the diagnostic meta-analysis and 14 studies from 13 articles 15,24,29,41,47,51,[63][64][65][66][67][68][69] were included in the prognostic meta-analysis. After screening the titles and abstracts, 476 articles were excluded because they were reviews (n = 18), meta-analyses (n = 5), nonhuman studies (n = 14), or studies irrelevant to our topic (n = 439).…”

Serum CA242: a biomarker for diagnosis, progression, and metastasis in multiple tumors

“…Furthermore, we also found that in all of the cancers listed in Table 4, the increase in the serum CA242 value for multiple metastases compared to NM was the largest, followed by the increases for liver metastasis and bone metastasis. Some studies have shown that the prognosis of gastric cancer with liver and bone metastasis is worse than that of any other subtype of gastric cancer, and the two organs even account for most metastatic sites [34,35]. Interestingly, we nd that similar situation also exist in colorectal cancer, lung cancer, pancreatic cancer, breast cancer and other tumors [36][37][38][39][40].We deem that the liver and bones may have microenvironments conducive to the synthesis and release of CA242 molecules in the ve "CA242 progression-positive tumors" or that the liver and bones enhance metastatic tumor cell growth so that more tumor cells produce more CA242, resulting in elevated levels.…”