The significance of batch and patch test method in establishing contact allergy to fragrance mix I—EDEN Fragrance Study Group

Abstract

Background: A fragrance mix consisting of eight separate fragrance ingredients (fragrance mix I [FM I]) is present in most baseline patch test series. Patch testing with the TRUE Test technique is considered to detect less contact allergy to FM I than testing with the Finn Chamber technique. Objective: To investigate the possible significance of batch and patch test method in establishing contact allergy to FM I. Methods: Three thousand one hundred and nineteen individuals representing a sample of the general population were patch tested with two batches of FM I with two patch test techniques at six dermatology clinics in five European countries. The TRUE Test technique and the Finn Chamber technique with pet. preparations were used. McNemar's test was used for statistical calculations.Results: The contact allergy prevalences varied between 0.7% and 2.6%. The patch tests with the mixes containing Evernia prunastri (oak moss) with a high content of chloratranol/atranol resulted in substantially more positive reactions than the corresponding tests with the mixes containing oak moss with a low content of chloratranol/atranol. The Finn Chamber technique detected significantly more contact allergic reactions than the TRUE Test technique (P < 0.001). Conclusion: The Finn Chamber technique detects more contact allergy to FM I than the TRUE Test technique. K E Y W O R D S allergic contact dermatitis, chloratranol/atranol, delayed hypersensitivity, Evernia prunastri, general population, oak moss

Simultaneous patch testing with fragrance mix I, fragrance mix II and their ingredients in southern Sweden between 2009 and 2015

“…It has been shown in patch tests and repeated open application tests that individuals sensitized to E. prunastri react to a lesser extent to the extracts with reduced atranol and chloroatranol levels . Furthermore, when patch testing was performed with FM I preparations containing E. prunastri extracts with reduced atranol and chloroatranol contents, statistically significantly fewer positive reactions were observed than for an identical (all other ingredients were from the same batches) FM I preparation containing an untreated E. prunastri extract . In the present study, both of the preparations made at our laboratory and those purchased from Chemotechnique Diagnostics contained untreated E. prunastri extracts .…”

Skin exposure to scented products used in daily life and fragrance contact allergy in the European general population ‐ The EDEN Fragrance Study

“…Study is a large cross-sectional epidemiological study on a random sample of adults from the European general population. [13][14][15][16][17][18][19][20] The aim of this study is to investigate the overall real life exposure to scented products used in daily life and to explore a relationship between exposure to scented products and fragrance contact allergy.…”

“…and FM II was tested in 14% pet., including its six separate ingredients. A detailed description hereof was published by Bruze et al 20 Patch tests were applied on the back for 48 hours under occlusion. Patch-test readings were performed at day (D)3.…”

Contact Allergy—Emerging Allergens and Public Health Impact

“…On the other hand, epoxycinnamyl alcohol and epoxycinnamal appeared not to be important when tested in 12 patients [80]. FM I contained in the TRUE Test™ system is significantly less sensitive than the pet.-based allergen, as repeatedly demonstrated, lately by the EDEN Fragrance Study [81], which needs to be taken into account when comparing test results obtained with different patch test systems. Moreover, all commercially available patch test preparations contain sorbitan sesquioleate (SSO) as an emulsifier.…”