The Sigma-1 receptor is an ER-localized type II membrane protein

Abstract

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Intracellular dynamics of the Sigma-1 receptor observed with super-resolution imaging microscopy

“…Sig1R was cloned in 1996 [ 38 ], while the crystal structure of full-length human Sig1R was published in 2016 [ 39 ]. Very recently, it was shown that Sig1R is an ER-localized type II membrane protein [ 40 ]. Nevertheless, there is no consensus about the dynamics of Sig1R within the cell.…”

“…Nevertheless, there is no consensus about the dynamics of Sig1R within the cell. On the other hand, there is quite a good consensus that Sig1R is an ER protein that acts as a molecular chaperone and forms cholesterol-enriched microdomains in the ER membrane [ 12 , 31 , 40 ]. However, most of the evidence regarding the dynamics of Sig1R obtained with different cell imaging techniques is indirect as dynamic changes occurring remain below the diffraction limit of light microscopy and therefore cannot be clearly characterized.…”

COP I and II dependent trafficking controls ER-associated degradation in mammalian cells

“…BirA and Sec-BirA plasmids were a kind gift from Gianlucca Petris and Oscar Burrone (ICGEB, Trieste, Italy) (21). H2a-BAP was cloned in pcDNA3.1 using restriction enzymes EcoR1 and XbaI for H2a(G78R) followed by a C-terminal BAP-SV5 tag using XbaI (39).…”

“…. H2a-BAP was cloned in pcDNA3.1 using restriction enzymes EcoR1 and XbaI for H2a(G78R) followed by a C-terminal BAP-SV5 tag using XbaI (39).…”

“…BAP-tagged H2a (H2a-BAP) was transfected and probed as described before (39). Briefly, H2a-BAP was cotransfected in HEK293 cells with an equal amount of plasmids carrying either BirA (cytosolic biotin ligase) or Sec-BirA (ER luminal biotin ligase).…”