The side-by-side exploratory test

Schaefer, Isabel C.; Siebel, Anna Maria; Piato, Ângelo; Bonan, Carla Denise; Vianna, Mônica Ryff Moreira Roca; Lara, Diogo R.

doi:10.1097/fbp.0000000000000145

Cited by 17 publications

(4 citation statements)

References 26 publications

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“…Distance between fish in a shoal is strongly dependent on context -for example, new environments and predatory threat 54 . In mammals, OT regulates anxiety and fear responses 5 , and pharmacological nonapeptide manipulation in adult zebrafish affects the response to predatory stimulus 42 .…”

Section: Discussionmentioning

confidence: 99%

Oxytocin Receptors Regulate Social Preference in Zebrafish

Landin

Hovey

et al. 2020

Sci Rep

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With a strong tendency to socialise, the zebrafish is a useful model to study social behaviour, with implications for better treatments of social impairments, for instance in autism spectrum disorders. Although oxytocin is crucial for social behaviour in mammals, the importance of the fish orthologue-isotocin or zebrafish oxytocin (zOT)-for social behaviour in zebrafish is unclear. The aims of this study were firstly, to elucidate the receptor specificity of zOT and the related vasotocin or zebrafish vasopressin (zVP; the orthologue of mammalian vasopressin) and the nonpeptidergic oxytocin receptor antagonist L-368,899, and secondly to investigate if L-368,899 inhibits social preference in zebrafish. The potencies of ligands were evaluated for zOT/zVP family receptors in HEK293 cells. Adult and larval zebrafish were treated with L-368,899 or vehicle and subsequently assessed for social behaviour and anxiety (adults only). The antagonist L-368,899 specifically inhibited the two zOT receptors, but not the two zVP-1 receptors. The antagonist decreased social preference in adult and larval zebrafish. It did not affect anxiety in adults. These results indicate that endogenous zOT, and possibly zVP, is involved in social behaviour in zebrafish via either or both of the two zOT receptors, and show promise for future explorations of the anatomy and evolution of networks underlying social behaviour.

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Section: Discussionmentioning

confidence: 99%

Oxytocin Receptors Regulate Social Preference in Zebrafish

Landin

Hovey

et al. 2020

Sci Rep

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“…Interestingly, researchers failed to observe a similar effect in another WT zebrafish strain, demonstrating natural variation in behavioral responses to neuroactive chemicals in different zebrafish strains (Scerbina et al, 2012). The common prescription drugs fluoxetine (Giacomini et al, 2016) and benzodiazepines (Giacomini et al, 2016; Schaefer et al, 2015) both inhibited shoaling. Fluoxetine also inhibited the offensive aggression behavior in dominant fish while suppressing freezing behavior in the subordinate fish (Theodoridi et al, 2017).…”

Section: Disease-relevant Social-deficit Models In Zebrafishmentioning

confidence: 99%

The zebrafish subcortical social brain as a model for studying social behavior disorders

Geng

Peterson

2019

Disease Models &Amp; Mechanisms

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Social behaviors are essential for the survival and reproduction of social species. Many, if not most, neuropsychiatric disorders in humans are either associated with underlying social deficits or are accompanied by social dysfunctions. Traditionally, rodent models have been used to model these behavioral impairments. However, rodent assays are often difficult to scale up and adapt to high-throughput formats, which severely limits their use for systems-level science. In recent years, an increasing number of studies have used zebrafish (Danio rerio) as a model system to study social behavior. These studies have demonstrated clear potential in overcoming some of the limitations of rodent models. In this Review, we explore the evolutionary conservation of a subcortical social brain between teleosts and mammals as the biological basis for using zebrafish to model human social behavior disorders, while summarizing relevant experimental tools and assays. We then discuss the recent advances gleaned from zebrafish social behavior assays, the applications of these assays to studying related disorders, and the opportunities and challenges that lie ahead.

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“…Social studies were conducted in mosquitofish (Gambusia affinis) (Polverino et al, 2013) and used to understand the impacts of domestication on sea bass (Dicentrarchus labrax) (Benhaïm et al, 2013). Pollutant effects on social behaviours were also investigated in Gilthead seabream (Sparus aurata) juveniles, where phenanthrene exposure decreased their social interaction (Correia et al, 2007) and with zebrafish exposed to various drugs (Schaefer et al, 2015). The goal of these experiments was to assess control FM behaviours using the light-dark preference and social interaction tests.…”

Section: Introductionmentioning

confidence: 99%

Maturation of behaviour in the fathead minnow

Vignet¹,

Parrott²

2017

Behavioural Processes

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Fathead minnow (FM, Pimephales promelas) are a species of small fish native to North America. Their small size, fast development, and ability to breed in the lab make them an ideal species to use in research, especially in toxicology. Behaviour in general is poorly studied in FM. The aim of this study was to characterize the normal behaviour of fathead minnow at 3 different stages of development in a light-dark box and in a social behaviour test. Fish larvae showed a preference for the light area, and then an increase in dark preference was seen as the fish aged. FM preferred to be with conspecifics at each age, but this preference was much stronger at the adult stage. The time of first entry into the conspecific area was reduced with increasing age of the fish. The time spent in the conspecific area increased between the juvenile and adult stage, and adults stayed more in this area when they entered it. Maturation of behavior in FM was demonstrated in our study. The FM is another good model fish to assess behavioral effects of chemicals, and this study helps to define the appropriate ages for behavioral studies with FM.

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The side-by-side exploratory test

Cited by 17 publications

References 26 publications

Oxytocin Receptors Regulate Social Preference in Zebrafish

Oxytocin Receptors Regulate Social Preference in Zebrafish

The zebrafish subcortical social brain as a model for studying social behavior disorders

Maturation of behaviour in the fathead minnow

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