The side activities of d-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating) from rabbit muscle: NADH-X formation

Glyceraldehyde-3-phosphate dehydrogenase catalyzed hydration of the 5-6 double bond of reduced β-nicotinamide adenine dinucleotide (βNADH). Formation of β-6-hydroxy-1,4,5,6-tetrahydronicotinamide adenine dinucleotide

“…The pH at which half the NADH is oxidized and half converted to (6HTN)AD is dependent on the polybasic anion present. The following anions are listed in order of their decreasing pH of 50% partition of NADH: pyrophosphate, 7.70; arsenate, 6.45; phosphate, 6.10; citrate, 6.05; acetate, 5.45 (Hilvers et al, 1966). Previously Rafter et al (1954) had observed that pyrophosphate increased the rate of formation of (6HTN)AD as did other polybasic anions.…”