The shunt pathway of mevalonate metabolism in the isolated perfused rat kidney.

Kopito, Ron R.; Murray, Donald M.; Story, David L.; Brunengraber, Henri

doi:10.1016/s0021-9258(17)43669-6

Cited by 13 publications

(30 citation statements)

References 45 publications

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“…In the normothermic perfusion group (Tables S3 and S4), the risk of bias was mainly unclear. Five studies (5/23, 22%) showed low risk of bias, when assessable 36,39,45,50,52 . Of these, two were recently published 39,50 .…”

Section: Resultsmentioning

confidence: 99%

“…Two studies investigated cholesterol synthesis using labeled mevalonate 28,36 . Brunengraber et al showed that 46% of R[3‐ 14 C]‐mevalonate was incorporated into lipids (saponifiable, digitonin‐precipitable sterols, and squalene and prenols), while 22% was secreted in the urine 28 .…”

Section: Resultsmentioning

confidence: 99%

“…35,38,48,51,55 Performance bias was rated as high in three studies, 31,34,40 low in one study, 42 and unclear in all others (26/30, 87%). [27][28][29][30]32,33,[35][36][37][38][39]41,[43][44][45][46][47][48][49][50][51][52][53][54][55] Random housing of animals was not performed in two studies (2/30, 7%) 31,40 and was not reported in the remaining st udies. [27][28][29][30][32][33][34][35][36][37][38][39][41][42][43]…”

Section: Quality and Risk Of Bias Assessmentmentioning

confidence: 99%

“…Five studies (5/23, 22%) showed low risk of bias, when assessable. 36,39,45,50,52 Of these, two were recently published. 39,50 However, in five articles, the risk of bias was assessed high on different domains.…”

Section: Quality and Risk Of Bias Assessmentmentioning

confidence: 99%

“…Normothermic perfusion was either pressure (13/23, 57%) 27,[29][30][31][32][33][34]36,46,47,50,52,53 or flow driven (4/23, 17%) 39,40,49,54 or not mentioned (6/23, 26%) 28,35,43,45,51,55 (Table 2). When pressure driven, target arterial pressure was uniformly set at 100 mm Hg, 27,[30][31][32][33][34]36,46,47,50,52,53 except in one study (120 mm Hg).…”

Section: Free Fatty Acid Metabolismmentioning

confidence: 99%

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Unraveling metabolism during kidney perfusion using tracer studies: a systematic review

et al. 2022

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Background Understanding kidney metabolism during perfusion is vital to further develop the technology as a preservation, viability assessment, and resuscitation platform. We reviewed the evidence on the use of labeled metabolites (tracers) to understand “on‐pump” kidney behavior. Methods PubMed, Embase, Web of Science, and Cochrane databases were systematically searched for studies evaluating metabolism of (non)radioactively labeled endogenous compounds during kidney perfusion. Results Of 5899 articles, 30 were included. All were animal studies [rat (70%), dog (13%), pig (10%), rabbit (7%)] perfusing but not transplanting kidneys. Perfusion took place at hypothermic (4–12°C) (20%), normothermic (35–40°C) (77%), or undefined temperatures (3%). Hypothermic perfusion used albumin or a clinical kidney preservation solution, mostly in the presence of oxygen. Normothermic perfusion was mostly performed with oxygenated crystalloids often containing glucose and amino acids with unclear partial oxygen tensions. Active metabolism of carbohydrate, amino acid, lipids, and large molecules was shown in hypothermic and normothermic perfusion. Production of macromolecules, such as prostaglandin, thromboxane, and vitamin D, takes place during normothermic perfusion. No experiments compared differences in metabolic activity between hypothermic and normothermic perfusion. One conference abstract showed increased anaerobic metabolism in kidneys donated after circulatory death by adding labeled glucose to hypothermically perfused human kidneys. Conclusions Tracer studies during kidney perfusion contribute to unraveling kidney metabolic behavior in pre‐clinical models. Whether findings are truly translational needs further investigation in large animal models of human kidneys. Furthermore, it is essential to better understand how ischemia changes this metabolic behavior.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%