The shorter zinc finger protein ZNF230 gene message is transcribed in fertile male testes and may be related to human spermatogenesis

Zhang, Sizhong; Qiu, Weimin; Wang, Hui; Zhang, Ge; Huang, Ming-kong; Xiao, Cuiying; Yang, Jun; Kamp, Christine; Huang, Xinli; Huellen, K.; Yue, Y.; Pan, Amy; Lebo, Roger V.; Milunsky, Aubrey; Vogt, Peter H.

doi:10.1042/0264-6021:3590721

Cited by 14 publications

(9 citation statements)

References 34 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ZNF230 gene, for example, encodes two different transcripts: the longer one of 4.4 kb is ubiquitously expressed, whereas the shorter form of 1 kb is detected only in the testis [73]. Interestingly, this latter form is absent in foetal testis and azoospermic patients, suggesting that ZNF230 might work in maintaning normal human male fertility.…”

Section: Krab Zinc Finger Proteins and Differentiationmentioning

confidence: 99%

KRAB-Zinc Finger Proteins: A Repressor Family Displaying Multiple Biological Functions

Cesaro²,

et al. 2013

View full text Add to dashboard Cite

Zinc finger proteins containing the Kruppel associated box (KRAB-ZFPs) constitute the largest individual family of transcriptional repressors encoded by the genomes of higher organisms. KRAB domain, positioned at the NH2 terminus of the KRAB-ZFPs, interacts with a scaffold protein, KAP-1, which is able to recruit various transcriptional factors causing repression of genes to which KRAB ZFPs bind. The relevance of such repression is reflected in the large number of the KRAB zinc finger protein genes in the human genome. However, in spite of their numerical abundance little is currently known about the gene targets and the physiological functions of KRAB- ZFPs. However, emerging evidence links the transcriptional repression mediated by the KRAB-ZFPs to cell proliferation, differentiation, apoptosis and cancer. Moreover, the fact that KRAB containing proteins are vertebrate-specific suggests that they have evolved recently, and that their key roles lie in some aspects of vertebrate development. In this review, we will briefly discuss some regulatory functions of the KRAB-ZFPs in different physiological and pathological states, thus contributing to better understand their biological roles.

show abstract

Section: Krab Zinc Finger Proteins and Differentiationmentioning

confidence: 99%

KRAB-Zinc Finger Proteins: A Repressor Family Displaying Multiple Biological Functions

Cesaro²,

et al. 2013

View full text Add to dashboard Cite

show abstract

“…This was submitted to GenBank (accession number: AY621088). Previous studies have shown that human ZNF230 has two transcripts ( Zhang et al , 2001 ). In mouse, there are three transcripts of znf230 ( Qiu et al 2003 ).…”

Section: Resultsmentioning

confidence: 99%

“…This suggested that ZNF230 may be involved in spermatogenesis, and loss of its expression may lead to azoospermia (Zhang et al , 2001). But so far, no clear biological function and mechanism had been elucidated.…”

Section: Introductionmentioning

confidence: 99%

“…The human ZNF230 , which maps to the short arm of chromosome 11 (11p15), encodes a C3HC4-type zinc finger protein motif (ring finger motif), and, consistent with a role in premeiotic or postmeiotic sperm development, one of its transcripts has been identified in abundance in the testicular tissue of fertile men, but neither in fetus nor in azoospermic patients. This suggested that ZNF230 may be involved in spermatogenesis, and loss of its expression may lead to azoospermia ( Zhang et al , 2001 ). But so far, no clear biological function and mechanism had been elucidated.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular cloning and expression analysis of a zebrafish novel zinc finger protein gene rnf141

et al. 2009

Self Cite

View full text Add to dashboard Cite

ZNF230 is a novel zinc finger gene cloned by our laboratory. In order to understand the potential functions of this gene in vertebrate development, we cloned the zebrafish orthologue of human ZNF230, named rnf141. The cDNA fragment of rnf141 was obtained by rapid amplification of cDNA ends (RACE). The open reading frame (ORF) encodes a polypeptide of 222 amino acids which shares 75.65% identity with the human ZNF230. RT-PCR analysis in zebrafish embryo and adult tissues revealed that rnf141 transcripts are maternally derived and that rnf141 mRNA has a broad distribution. Zygotic rnf141 message is strongly localized in the central nervous system, as shown by whole-mount in situ hybridization. Knockdown and over expression of rnf141 can induce abnormal phenotypes, including abnormal development of brain, as well as yolk sac and axis extendsion. Marker gene analysis showed that rnf141 may play a role in normal dorsoventral patterning of zebrafish embryos, suggesting that rnf141 may have a broad function during early development of vertebrates.

show abstract

“…The human gene ZNF230 (also named RNF141 ), which encodes a type of RING (Really Interesting New Gene) finger protein, was first identified in our laboratory to be restrictively expressed in the testicular tissue of fertile men ( Zhang et al , 2001 ). RING finger proteins, a sub-family of zinc finger proteins (ZFP), often contain the cysteine-rich CX 2 CX (9–39) CX (1–3) HX (2–3) CX 2 CX (4–48) CX 2 C domain and are involved in a variety of biological processes, including transcriptional regulation, signal transduction, cell apoptosis and protein ubiquitination ( Borden, 2000 ; Joazeiro and Weissman, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility

Tao¹,

Lu²,

Yang³

et al. 2014

Genet. Mol. Biol.

Self Cite

View full text Add to dashboard Cite

The mouse testis-enriched Znf230 gene, which encodes a type of RING finger protein, is present primarily in the nuclei of spermatogonia, the acrosome and the tail of spermatozoa. To investigate the role of Znf230 in spermatogenesis, we generated Znf230-deficient mice by disrupting Znf230 exon-5 and exon-6 using homologous recombination. The homozygous Znf230-knockout (KO) mice did not exhibit Znf230 mRNA expression and Znf230 protein production. Znf230 KO mice exhibited no obvious impairment in body growth or fertility. Male Znf230 KO mice had integral reproductive systems and mature sperm that were regular in number and shape. The developmental stages of male germ cells of Znf230 KO mice were also normal. We further examined variations in the transcriptomes of testicular tissue between Znf230 KO and wild-type mice through microarray analysis. The results showed that the mRNA level of one unclassified transcript 4921513I08Rik was increased and that the mRNA levels of three other transcripts, i.e., 4930448A20Rik, 4931431B13Rik and potassium channel tetramerisation domain containing 14(Kctd14), were reduced more than two-fold in Znf230 KO mice compared with wild-type mice. Using our current examination techniques, these findings suggested that Znf230 deficiency in mice may not affect growth, fertility or spermatogenesis.

show abstract

The shorter zinc finger protein ZNF230 gene message is transcribed in fertile male testes and may be related to human spermatogenesis

Cited by 14 publications

References 34 publications

KRAB-Zinc Finger Proteins: A Repressor Family Displaying Multiple Biological Functions

KRAB-Zinc Finger Proteins: A Repressor Family Displaying Multiple Biological Functions

Molecular cloning and expression analysis of a zebrafish novel zinc finger protein gene rnf141

Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility

Contact Info

Product

Resources

About