The short term High Fat Diet sparks gut microbiota associated metabolic syndrome pathogenesis and impact of Amoxicillin intervention in mice

Abstract

Background: Knowledge about alteration in gut microbiota on a high-fat diet (HFD) and common antibiotics for a short period is little known. The study was undertaken to evaluate the changes in the microbiota profile in HFD fed mice followed by amoxicillin intervention. Results: For two weeks, the mice were fed with HFD followed by amoxicillin for one week. Animals were evaluated for haemato-biochemical, histopathological and 16S rRNA sequencing followed by bioinformatics analysis to know any changes in gut microbiota ecology. Amoxicillin treatment for a short duration had marked the remarkable remodelling of gut microbiota that directly linked to beneficial impact by countering the pathogenesis of the metabolic syndrome besides overgrowth of particular opportunistic pathogens of gut inflammatory diseases. Amoxicillin treatment significantly decreased blood glucose and a slight elevation in cholesterol levels on blood biochemistry analysis. No marked pathological changes were observed in HFD fed mice. Conclusions: Our results suggest that Amoxicillin treatment had a beneficial influence on the obese related metabolic syndrome with the risk of hypercholesterolemia and some gut-resistant intestinal pathobionts.