The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde[W]

González-Guzmán, Miguel; Apostolova, Nadezda; Bellés, José M.; Barrero, José M.; Piqueras, Pedro; Ponce, Marı́a Rosa; Micol, José Luis; Serrano, Ramón; Rodrı́guez, Pedro L.

doi:10.1105/tpc.002477

Cited by 439 publications

(373 citation statements)

References 55 publications

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“…We then investigated the phenotype of other ABA-related mutants. Similar to nced3, the ABA-deficient mutant aba2 fails to synthesize ABA, as it lacks the short-chain alcohol dehydrogenase ABA2 responsible for the conversion of xanthoxin to abscisic aldehyde (González-Guzmán et al, 2002;Schwartz et al, 1997). As observed for nced3, aba2 also failed to activate starch degradation and showed no or much reduced maltose accumulation in response to osmotic stress (Supplemental Figure 10).…”

Section: Carbon Export To the Root And Osmolyte Accumulation During Omentioning

confidence: 79%

Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

et al. 2016

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Starch serves functions that range over a timescale of minutes to years, according to the cell type from which it is derived. In guard cells, starch is rapidly mobilized by the synergistic action of b-AMYLASE1 (BAM1) and a-AMYLASE3 (AMY3) to promote stomatal opening. In the leaves, starch typically accumulates gradually during the day and is degraded at night by BAM3 to support heterotrophic metabolism. During osmotic stress, starch is degraded in the light by stress-activated BAM1 to release sugar and sugar-derived osmolytes. Here, we report that AMY3 is also involved in stress-induced starch degradation. Recently isolated Arabidopsis thaliana amy3 bam1 double mutants are hypersensitive to osmotic stress, showing impaired root growth. amy3 bam1 plants close their stomata under osmotic stress at similar rates as the wild type but fail to mobilize starch in the leaves. 14 C labeling showed that amy3 bam1 plants have reduced carbon export to the root, affecting osmolyte accumulation and root growth during stress. Using genetic approaches, we further demonstrate that abscisic acid controls the activity of BAM1 and AMY3 in leaves under osmotic stress through the AREB/ABF-SnRK2 kinase-signaling pathway. We propose that differential regulation and isoform subfunctionalization define starch-adaptive plasticity, ensuring an optimal carbon supply for continued growth under an ever-changing environment.

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Section: Carbon Export To the Root And Osmolyte Accumulation During Omentioning

confidence: 79%

Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

et al. 2016

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“…ABA plays a critical role promoting inhibition of both seed germination and early seedling growth under high osmoticum (Gonzalez-Guzman et al, 2002). Thus, whereas ABA-hypersensitive mutants are generally more sensitive than wild type to the inhibition of seed germination promoted by osmotic stress (Saez et al, 2004), both ABA-deficient and ABA-insensitive mutants are more tolerant to osmotic stress at this stage (Leon-Kloosterziel et al, 1996;Gonzalez-Guzman et al, 2002).…”

Section: Identification and Characterization Of Knockout Alleles Of Abi1mentioning

confidence: 99%

“…Thus, whereas ABA-hypersensitive mutants are generally more sensitive than wild type to the inhibition of seed germination promoted by osmotic stress (Saez et al, 2004), both ABA-deficient and ABA-insensitive mutants are more tolerant to osmotic stress at this stage (Leon-Kloosterziel et al, 1996;Gonzalez-Guzman et al, 2002). Dose-response analyses of germination and early growth in media supplemented with increasing concentrations of NaCl or mannitol were performed for abi1-2 and abi1-3 (Fig.…”

Section: Identification and Characterization Of Knockout Alleles Of Abi1mentioning

confidence: 99%

Enhancement of Abscisic Acid Sensitivity and Reduction of Water Consumption in Arabidopsis by Combined Inactivation of the Protein Phosphatases Type 2C ABI1 and HAB1

et al. 2006

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Abscisic acid (ABA) plays a key role in plant responses to abiotic stress, particularly drought stress. A wide number of ABAhypersensitive mutants is known, however, only a few of them resist/avoid drought stress. In this work we have generated ABA-hypersensitive drought-avoidant mutants by simultaneous inactivation of two negative regulators of ABA signaling, i.e. the protein phosphatases type 2C (PP2Cs) ABA-INSENSITIVE1 (ABI1) and HYPERSENSITIVE TO ABA1 (HAB1). Two new recessive loss-of-function alleles of ABI1, abi1-2 and abi1-3, were identified in an Arabidopsis (Arabidopsis thaliana) T-DNA collection. These mutants showed enhanced responses to ABA both in seed and vegetative tissues, but only a limited effect on plant drought avoidance. In contrast, generation of double hab1-1 abi1-2 and hab1-1 abi1-3 mutants strongly increased plant responsiveness to ABA. Thus, both hab1-1 abi1-2 and hab1-1 abi1-3 were particularly sensitive to ABA-mediated inhibition of seed germination. Additionally, vegetative responses to ABA were reinforced in the double mutants, which showed a strong hypersensitivity to ABA in growth assays, stomatal closure, and induction of ABA-responsive genes. Transpirational water loss under drought conditions was noticeably reduced in the double mutants as compared to single parental mutants, which resulted in reduced water consumption of whole plants. Taken together, these results reveal cooperative negative regulation of ABA signaling by ABI1 and HAB1 and suggest that fine tuning of ABA signaling can be attained through combined action of PP2Cs. Finally, these results suggest that combined inactivation of specific PP2Cs involved in ABA signaling could provide an approach for improving crop performance under drought stress conditions.

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“…These mutants have been identified on the basis of their wilty phenotype or seed germination characteristics (Koornneef et al, 1982;Leon-Kloosterziel et al, 1996). Particularly, mutants able to germinate and carry out early growth in medium containing a high-NaCl concentration (sre mutants for salt resistant) were found to be allelic to ABA-deficient mutants (Gonzalez-Guzman et al, 2002). Thus, the sre1 mutant was found to be allelic to aba2-1 mutant (Gonzalez-Guzman et al, 2002).…”

mentioning

confidence: 99%

“…Particularly, mutants able to germinate and carry out early growth in medium containing a high-NaCl concentration (sre mutants for salt resistant) were found to be allelic to ABA-deficient mutants (Gonzalez-Guzman et al, 2002). Thus, the sre1 mutant was found to be allelic to aba2-1 mutant (Gonzalez-Guzman et al, 2002). In this work, we report the characterization of two new sre mutants in a Columbia (Col) genetic background, sre2-1 and sre2-2, which were found to be allelic to aao3-1.…”

mentioning

confidence: 99%

Two New Alleles of the abscisic aldehyde oxidase 3 Gene Reveal Its Role in Abscisic Acid Biosynthesis in Seeds

et al. 2004

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The abscisic aldehyde oxidase 3 (AAO3) gene product of Arabidopsis catalyzes the final step in abscisic acid (ABA) biosynthesis. An aao3-1 mutant in a Landsberg erecta genetic background exhibited a wilty phenotype in rosette leaves, whereas seed dormancy was not affected (Seo et al., 2000a). Therefore, it was speculated that a different aldehyde oxidase would be the major contributor to ABA biosynthesis in seeds (Seo et al., 2000a). Through a screening based on germination under high-salt concentration, we isolated two mutants in a Columbia genetic background, initially named sre2-1 and sre2-2 (for salt resistant). Complementation tests with different ABA-deficient mutants indicated that sre2-1 and sre2-2 mutants were allelic to aao3-1, and therefore they were renamed as aao3-2 and aao3-3, respectively. Indeed, molecular characterization of the aao3-2 mutant revealed a T-DNA insertional mutation that abolished the transcription of AAO3 gene, while sequence analysis of AAO3 in aao3-3 mutant revealed a deletion of three nucleotides and several missense mutations. Physiological characterization of aao3-2 and aao3-3 mutants revealed a wilty phenotype and osmotolerance in germination assays. In contrast to aao3-1, both aao3-2 and aao3-3 mutants showed a reduced dormancy. Accordingly, ABA levels were reduced in dry seeds and rosette leaves of both aao3-2 and aao3-3. Taken together, these results indicate that AAO3 gene product plays a major role in seed ABA biosynthesis.

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The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde[W]

Cited by 439 publications

References 55 publications

Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

Enhancement of Abscisic Acid Sensitivity and Reduction of Water Consumption in Arabidopsis by Combined Inactivation of the Protein Phosphatases Type 2C ABI1 and HAB1

Two New Alleles of the abscisic aldehyde oxidase 3 Gene Reveal Its Role in Abscisic Acid Biosynthesis in Seeds

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