The sheep miRNAome: Characterization and distribution of miRNAs in 21 tissues

Bilbao-Arribas, Martin; Guisasola-Serrano, Aitor; Varela-Martínez, Endika; Jugo, Begoña M.

doi:10.1016/j.gene.2022.146998

Cited by 2 publications

(2 citation statements)

References 70 publications

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“…Moreover, comparing the spleen with previously analysed tissues from the same group of animals 14,15 the overlap among the different datasets was quite high with 110 miRNAs in common. The spleen showed the highest number of specific miRNAs (50% of the detected miRNAs in this tissue) and members of the let-7 family and miR-26a were highly expressed in all tissues in a consistent way 18 . www.nature.com/scientificreports/ Among the 12 miRNAs dysregulated by both treatments, the downregulated miR-148b was the most significantly differentially expressed miRNA.…”

Section: Discussionmentioning

confidence: 69%

Identification and characterization of miRNAs in spleens of sheep subjected to repetitive vaccination

Varela-Martínez

Bilbao-Arribas

Abendaño

et al. 2023

Sci Rep

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Accumulative evidence has shown that short non-coding RNAs such as miRNAs can regulate the innate and adaptive immune responses. Aluminium hydroxide is a commonly used adjuvant in human and veterinary vaccines. Despite its extended use, its mechanism of action is not fully understood and very few in vivo studies have been done to enhance understanding at the molecular level. In this work, we took advantage of a previous long-term experiment in which lambs were exposed to three different treatments by parallel subcutaneous inoculations with aluminium-containing commercial vaccines, an equivalent dose of aluminium or mock injections. Spleen samples were used for miRNA-seq. A total of 46 and 16 miRNAs were found differentially expressed when animals inoculated with commercial vaccines or the adjuvant alone were compared with control animals, respectively. Some miRNAs previously related to macrophage polarization were found dysregulated exclusively by the commercial vaccine treatment but not in the aluminium inoculated animals. The dysregulated miRNAs in vaccine group let-7b-5p, miR-29a-3p, miR-27a and miR-101-3p are candidates for further research, since they may play key roles in the immune response induced by aluminium adjuvants added to vaccines. Finally, protein–protein interaction network analysis points towards leucocyte transendothelial migration as a specific mechanism in animals receiving adjuvant only.

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Section: Discussionmentioning

confidence: 69%

Identification and characterization of miRNAs in spleens of sheep subjected to repetitive vaccination

Varela-Martínez

Bilbao-Arribas

Abendaño

et al. 2023

Sci Rep

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“…In order to identify a broader set of differentially expressed miRNAs, we conducted a comparison of the obtained clean reads with the Ensembl database [ 11 , 12 ], resulting in the identification of 1,606 up-regulated miRNAs and 693 down-regulated miRNAs (Additional file 13 : Table S10). Among these, a significant subset of 885 up-regulated miRNAs (55.1% of 1,606) were found in the the Dlk1 - Gtl2 imprinted region (Fig.…”

Section: Resultsmentioning

confidence: 99%

Imprinted Dlk1-Gtl2 cluster miRNAs are potential epigenetic regulators of lamb fur quality

Zhang,

Wang,

Cai

et al. 2023

BMC Genomics

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Background Tan and Hu sheep are well-known local breeds in China, producing lamb fur with unique ornamental and practical values highly appreciated by consumers worldwide. Fur quality is optimal at one month of age and gradually declines with time. Despite active research on its genetic mechanism using transcriptomic and whole genome bisulfite sequencing analysis, the main effective gene locus has not been found, and its regulatory mechanism is still unclear, which limits the breeding and improvement of fur traits. Results Scapular skin samples from newborn (1-month old) and adult (24-month old) Tan sheep were utilized for small ribonucleic acid (RNA) sequencing Principal Component Analysis (PCA) showed that the newborn and adult groups were completely separated. Differential expression analysis of micro-RNAs (miRNAs) identified 32 up-regulated miRNAs and 48 down-regulated miRNAs in the newborn groups. All up-regulated miRNAs were located in the imprinted. Dlk1-Gtl2 locus on chromosome 18, whereas all down-regulated miRNAs were distributed across the sheep chromosomes, without a clear pattern of positional consistency. Further, by systematically analyzing the target genes and signaling pathways of all 32 up-regulated miRNAs, we found that the PI3K-AKT signaling pathway has the potential to be targeted and regulated by most of the miRNAs in the Dlk1-Gtl2 region. In addition, we also re-analyzed miRNA sequencing data from public databases on Hu lambs (full sibling Hu lambs with high- and low-quality fur characteristics). Again, it was found that most of the up-regulated miRNAs in lambs with high-quality fur were also located in the Dlk1-Gtl2 region, whereas this patter was not present for down-regulated miRNAs. Conclusion Sequencing of miRNAs in conjunction with public databases was employed to identify miRNAs within the imprinted Dlk1-Gtl2 region on chromosome 18, suggesting their potential roles as epigenetic regulators of fur traits. Small RNAs located at the Dlk1-Gtl2 locus were identified as having the potential to systematically regulate the PI3K-AKT signaling pathway, thereby indicating the relevance of the Dlk1-Gtl2/PI3K-AKT axis in the context of fur traits. Selection of parental specific expressed imprinted genes in the process of conserving and exploiting lamb fur traits should be emphasized.

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The sheep miRNAome: Characterization and distribution of miRNAs in 21 tissues

Cited by 2 publications

References 70 publications

Identification and characterization of miRNAs in spleens of sheep subjected to repetitive vaccination

Identification and characterization of miRNAs in spleens of sheep subjected to repetitive vaccination

Imprinted Dlk1-Gtl2 cluster miRNAs are potential epigenetic regulators of lamb fur quality

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