The sex-determining region of the human Y chromosome encodes a finger protein

Page, David C.; Mosher, Rebecca A.; Simpson, Elizabeth M.; Fisher, Elizabeth; Mardon, Graeme; Pollack, Jonathan R.; McGillivray, Barbara; Chapelle, Albert de la; Brown, Laura G.

doi:10.1016/0092-8674(87)90595-2

Cited by 820 publications

(382 citation statements)

References 83 publications

(88 reference statements)

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“…In Mus musculus, Sry lies within 2.8 kb of unique sequence at the center of a large inverted repeat (Gubbay et al 1992), a possible relic of multiple copies of Sry in the Mus lineage. Two closely linked genes, zinc finger protein, Zfy (Page et al 1987), and a gene of unknown function, Ube1Y (Mitchell et al 1991), have also multiplied on the Mus musculus Y chromosome. Zfy is present in two copies in Mus musculus (Mardon et al 1989;Nagamine et al 1989) and has amplified up to 26 times on the Y chromosome in other rodents Lau et al 1992;Nagamine 1994).…”

Section: Discussionmentioning

confidence: 99%

Evidence for multiple functional copies of the male sex-determining locus, sry, in African murine rodents

Lundrigan

Tucker

1997

J Mol Evol

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Abstract. Southern hybridization data suggest that the male sex-determining locus, Sry, is often duplicated in rodents. Here we explore DNA sequence evolution of orthologous and paralogous copies of Sry isolated from six species of African murines. PCR amplification followed by direct sequencing revealed from two to four copies of Sry per species. All copies include a long open reading frame, with a stop codon that coincides closely with the stop codon of the house mouse, Mus musculus, a species known to have a single copy of Sry. A phylogenetic analysis suggests that there are at least seven paralogous copies of Sry in this group of rodents. Putative orthologues are identical; sequence divergence among putative paralogues ranges from 1 to 8% (excluding the CAG repeat), with much lower levels of divergence in the high-mobility group (HMG-box) region than in the C-terminal region. A high proportion of nucleotide substitutions in both regions result in aminoacid replacement. The long open reading frame, conserved HMG-box, and pattern of evolution of the putative paralogues suggest that they are functional.

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Section: Discussionmentioning

confidence: 99%

Evidence for multiple functional copies of the male sex-determining locus, sry, in African murine rodents

Lundrigan

Tucker

1997

J Mol Evol

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“…Owing to methodological difficulties this could not be applied extensively to other poecilüd fishes where genetic heterogamety had not been demonstrated. Recently the so-called ZFY sequence, a "zinc finger" protein encoded by the human Y (Page et al 1987), has been postulated to be associated with the sex chromosomes of many diversified vertebrates. This view, however, remains controversial because of reports on marsupials (Sinclair et al 1988) and reptiles (Bull et al 1988).…”

Section: Discussionmentioning

confidence: 99%

Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences

Nanda

Schartl²,

Feichtinger

et al. 1992

Chromosoma

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Abstract. Animal sex chromosome evolution has started on different occasions with a homologous pair of autosomes leading to morphologically differentiated gonosomes. In contrast to other vertebrate classes, among fishes cytologically dernonstrahle sex chromosomes are rare. In reptiles, certain motifs of simple tandemly repeated DNA sequences like (gata)n/(gaca)m are associated with the constitutive heterochromatin of sex chromosomes. In this study a panel of simple repetitive sequence probes was hybridized to restriction enzyme digested genomic DNA of poeciliid fishes. Apparent male heterogamety previously established by genetic experiments in Poecilia reticulata (guppy) was correlated with male-specific hybridization using the (GACA) 4 probe. The (GAT A) 4 oligonucleotide identifies certain male guppies by a Y chromosomal polymorphism in the outbred population. In cantrast none of the genetically defined heterogametic situations in Xiphophorus could be verified consistently using the collection of simple repetitive sequence probes. Only individuals from particular populations produced sex-specific patterns of hybridization with (GAT A) 4 • Additional poeciliid species (P. sphenops, P. velifera) harbour different sex-specifically organized simple repeat motifs. The observed sex-specific hybridization patterns were substantiated by banding analyses of the karyotypes and by in situ hybridization using the (GACA) 4 probe.

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“…Although previously thought to have a function in testis determination, such a role has now been excluded [1][2][3]. It has been suggested that the Z f y l gene may have a function in spermatogenesis, perhaps as a transcription factor [4].…”

Section: Discussionmentioning

confidence: 99%

“…The ZFY gene was originally identified as a human Y chromosomal gene that was a candidate for the testis-determining factor [1]. Although a primary role in sex determination has now been ruled out [2,3] the conservation of this gene on the Y chromosome of placental mammals suggests it has some important male-specific function, perhaps in spermatogenesis [4].…”

Section: Introductionmentioning

confidence: 99%

Zfy1 encodes a nuclear sequence‐specific DNA binding protein

1995

FEBS Letters

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Zfyl is a mouse Y chromosomal gene encoding a zinc finger protein which is thought to have some function during spermatogenesis. Here we show that, when introduced into tissue culture cells, Zfyl is targeted to the nucleus. Two independent signals are present within the protein for nuclear localization. This nuclear Zfyl protein is able to bind strongly to DNA-ceHulose and, using site-selection assays, we have identified specific Zfyl DNA binding sites. Taken together these results suggest that Zfyl is a nuclear-located sequence-specific DNA binding protein which functions during spermatogenesis.

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The sex-determining region of the human Y chromosome encodes a finger protein

Cited by 820 publications

References 83 publications

Evidence for multiple functional copies of the male sex-determining locus, sry, in African murine rodents

Evidence for multiple functional copies of the male sex-determining locus, sry, in African murine rodents

Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences

Zfy1 encodes a nuclear sequence‐specific DNA binding protein

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