The Sex-Determining Locus in the Tiger Pufferfish,<i>Takifugu rubripes</i>

Kikuchi, Kiyoshi; Wang, Kai; Hosokawa, Ayumi; Mizuno, Naoki; Suetake, Hiroaki; Asahina, Kiyoshi; Suzuki, Yuzuru

doi:10.1534/genetics.106.069278

Cited by 69 publications

(42 citation statements)

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“…Our data suggest the synteny of the turbot LG5 distal interval with Ola LG4, Gac LG8, and Tni LG1. The lack of homology of turbot LG5 markers with the sex chromosomes of medaka (LG1; Matsuda et al 2002), stickleback (LG19; Peichel et al 2004), or fugu (scaffolds anchored to LG19; Kikuchi et al 2007) suggests that the sex chromosome of turbot evolved independently from that of these three model species. Nevertheless, turbot LG5 markers could be indirectly linked to the Oreochromis spp.…”

Section: Discussionmentioning

confidence: 99%

“…As a consequence, the SD master gene in fish can vary among related species (Kondo et al 2003;Tanaka et al 2007;Alfaqih et al 2009). In this sense, fish represent an attractive model for studying the evolution of SD mechanisms and sex chromosomes (Peichel et al 2004;Kikuchi et al 2007).…”

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confidence: 99%

“…Also, microarrays constructed from gonadal ESTs have been applied to detect differentially expressed genes in the process of gonadal differentiation (Baron et al 2005). Further, the increased genomic resources in model and aquaculture species have allowed the development of both comparative genomics (Woram et al 2003;Kikuchi et al 2007;Tripathi et al 2009) and candidate gene (Shirak et al 2006;Alfaqih et al 2009) strategies to identify and characterize the SD region in fish. This has permitted the identification of the SD region in eight fish, including both model and aquaculture species (reviewed in Penman and Piferrer 2008).…”

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confidence: 99%

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Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

et al. 2009

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Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbot (Scophthalmus maximus) is a flatfish of great commercial value, where a strong sexual dimorphism exists for growth rate. Following a QTL and marker association approach in five families and a natural population, we identified the main SD region of turbot at the proximal end of linkage group (LG) 5, close to the SmaUSC-E30 marker. The refined map of this region suggested that this marker would be 2.6 cM and 1.4 Mb from the putative SD gene. This region appeared mostly undifferentiated between males and females, and no relevant recombination frequency differences were detected between sexes. Comparative genomics of LG5 marker sequences against five model species showed no similarity of this chromosome to the sex chromosomes of medaka, stickleback, and fugu, but suggested a similarity to a sex-associated QTL from Oreochromis spp. The segregation analysis of the closest markers to the SD region demonstrated a ZW/ZZ model of sex determination in turbot. A small proportion of families did not fit perfectly with this model, which suggests that other minor genetic and/or environmental factors are involved in sex determination in this species.

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Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

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“…Another pleuronectiform, the half-smooth tongued sole, has female heterogamety, and many genes present only in females have been identified through a BAC library screen, suggesting W linkage (Shao et al 2010). Among fish whose genomes have been sequenced, the pufferfish (Takifugu rubripes, order Tetraodontiformes) has a male-heterozygous region (Kikuchi et al 2007). In the zebrafish (Danio rerio, Cypriniformes), sex is determined by a combination of genetic and environmental influences (Siegfried and Nusslein-Volhard 2008), while among its relatives, the goldfish has an XY system of GSD (Zhang and Wu 1985) and Leporinus (Characiformes) has a strongly heteromorphic ZW system, with largely heterochromatic W chromosomes, which have probably undergone W-autosome translocations (Parise-Maltempi et al 2007).…”

Section: Genetic Mapping Of Sex-determining Regionsmentioning

confidence: 99%

The Birds and the Bees and the Flowers and the Trees: Lessons from Genetic Mapping of Sex Determination in Plants and Animals

2010

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The ability to identify genetic markers in nonmodel systems has allowed geneticists to construct linkage maps for a diversity of species, and the sex-determining locus is often among the first to be mapped. Sex determination is an important area of study in developmental and evolutionary biology, as well as ecology. Its importance for organisms might suggest that sex determination is highly conserved. However, genetic studies have shown that sex determination mechanisms, and the genes involved, are surprisingly labile. We review studies using genetic mapping and phylogenetic inferences, which can help reveal evolutionary pattern within this lability and potentially identify the changes that have occurred among different sex determination systems. We define some of the terminology, particularly where confusion arises in writing about such a diverse range of organisms, and highlight some major differences between plants and animals, and some important similarities. We stress the importance of studying taxa suitable for testing hypotheses, and the need for phylogenetic studies directed to taxa where the patterns of changes can be most reliably inferred, if the ultimate goal of testing hypotheses regarding the selective forces that have led to changes in such an essential trait is to become feasible.

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“…Similarly, the sex-determining loci of different salmonids (Phillips et al 2005(Phillips et al , 2007Artieri et al 2006) and of two species of stickleback (Gasterosteus) have apparently evolved from loci originating on distinct ancestral chromosomes (Woram et al 2003;Charlesworth 2004;Peichel et al 2004). In the threespine stickleback Gasterosteus aculeatus (Peichel et al 2004), the tiger pufferfish Takifugu rubripes (Kikuchi et al 2007), the rainbow trout (Alfaqih et al 2009), and the platyfish Xiphophorus maculatus (Volff and Schartl 2002), the SDL could be mapped to a specific linkage group that represents the differentiating Y chromosome. Fine mapping of these loci is impeded by suppression of meiotic recombination and by frequent occurrence of repetitive sequences in the gonosomal region of the Y chromosome (Nanda et al 1993;Peichel et al 2004).…”

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Linkage Analysis Reveals the Independent Origin of Poeciliid Sex Chromosomes and a Case of Atypical Sex Inheritance in the Guppy (Poecilia reticulata)

et al. 2009

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Among different teleost fish species, diverse sex-determining mechanisms exist, including environmental and genetic sex determination, yet chromosomal sex determination with male heterogamety (XY) prevails. Different pairs of autosomes have evolved as sex chromosomes among species in the same genus without evidence for a master sex-determining locus being identical. Models for evolution of Y chromosomes predict that male-advantageous genes become linked to a sex-determining locus and suppressed recombination ensures their co-inheritance. In the guppy, Poecilia reticulata, a set of genes responsible for adult male ornaments are linked to the sex-determining locus on the incipient Y chromosome. We have identified .60 sex-linked molecular markers to generate a detailed map for the sex linkage group of the guppy and compared it with the syntenic autosome 12 of medaka. We mapped the sex-determining locus to the distal end of the sex chromosome. We report a sex-biased distribution of recombination events in female and male meiosis on sex chromosomes. In one mapping cross, we observed sex ratio and male phenotype deviations and propose an atypical mode of genetic sex inheritance as its basis.

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The Sex-Determining Locus in the Tiger Pufferfish,Takifugu rubripes

Abstract: The tiger pufferfish (fugu), Takifugu rubripes, is a model fish that has had its genome entirely sequenced. By performing genomewide linkage analyses, we show that the sex of fugu is determined by a single chromosomal region on linkage group 19 in an XX-XY system.

Cited by 69 publications

References 13 publications

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

The Birds and the Bees and the Flowers and the Trees: Lessons from Genetic Mapping of Sex Determination in Plants and Animals

Linkage Analysis Reveals the Independent Origin of Poeciliid Sex Chromosomes and a Case of Atypical Sex Inheritance in the Guppy (Poecilia reticulata)

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