The Serotonin 5-HT7Dro Receptor Is Expressed in the Brain of Drosophila, and Is Essential for Normal Courtship and Mating

Becnel, Jaime; Johnson, Oralee; Luo, Jiangnan; Nässel, Dick R.; Nichols, Charles D.

doi:10.1371/journal.pone.0020800

Cited by 106 publications

(120 citation statements)

References 62 publications

(75 reference statements)

Supporting

Mentioning

111

Contrasting

Unclassified

Order By: Relevance

“…We, and others, have previously used the assays described here to gather data to examine genes related to fly behaviors and to human neurodegenerative diseases modeled in Drosophila [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] . For the crawling assay, careful selection of 3 rd instar larvae is a critical step.…”

Section: Discussionmentioning

confidence: 99%

“…This behavior has been used to examine aspects of behaviors relevant to human diseases, and involves sensory processing (olfactory, visual, acoustic) in addition to fine motor control 18 . When a male notices a female, he initiates the courtship ritual that progresses in a stereotype pattern beginning with orientation behavior (turning toward and chasing the female).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Methods to Assay Drosophila Behavior

Nichols

Becnel

Pandey

2012

JoVE

Self Cite

201

169

View full text Add to dashboard Cite

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular disease and various neurological diseases 1 . We have optimized simple and robust behavioral assays for determining larval locomotion, adult climbing ability (RING assay), and courtship behaviors of Drosophila. These behavioral assays are widely applicable for studying the role of genetic and environmental factors on fly behavior. Larval crawling ability can be reliably used for determining early stage changes in the crawling abilities of Drosophila larvae and also for examining effect of drugs or human disease genes (in transgenic flies) on their locomotion. The larval crawling assay becomes more applicable if expression or abolition of a gene causes lethality in pupal or adult stages, as these flies do not survive to adulthood where they otherwise could be assessed. This basic assay can also be used in conjunction with bright light or stress to examine additional behavioral responses in Drosophila larvae. Courtship behavior has been widely used to investigate genetic basis of sexual behavior, and can also be used to examine activity and coordination, as well as learning and memory. Drosophila courtship behavior involves the exchange of various sensory stimuli including visual, auditory, and chemosensory signals between males and females that lead to a complex series of well characterized motor behaviors culminating in successful copulation. Traditional adult climbing assays (negative geotaxis) are tedious, labor intensive, and time consuming, with significant variation between different trials [2][3][4] . The rapid iterative negative geotaxis (RING) assay 5 has many advantages over more widely employed protocols, providing a reproducible, sensitive, and high throughput approach to quantify adult locomotor and negative geotaxis behaviors. In the RING assay, several genotypes or drug treatments can be tested simultaneously using large number of animals, with the high-throughput approach making it more amenable for screening experiments. Video LinkThe 3. Incubate bottle for 3-4 days, or until third instar larvae are visible. 4. Add 50 -100 ml of 20% sucrose to the bottle with larvae and let sit for 20 minutes. Larvae will float to the top. 5. Collect larvae using a 25 ml serological pipette with the tip cut off, and place into a mesh basket. 6. Wash larvae in the mesh basket two times with deionized H 2 O. Larvae are now ready for the experiments. To treat larvae with drug1. Use a brush to transport the desired number of larvae to a 5 ml beaker containing a solution 5% sucrose + drug. 2. Let larvae feed for at least 15 minutes. 3. Pour drug-treated larvae into a mesh basket and rinse. They are now ready to use. Locomotor Assay (measuring total distance travelled or body wall contractions)1. Use a brush to transport individual larva to a: 1. 15 cm Petri dish containing 2% agarose (previously poured and allowed to harden) over graph paper with a 0.2 cm 2 grid.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Methods to Assay Drosophila Behavior

Nichols

Becnel

Pandey

2012

JoVE

Self Cite

201

169

View full text Add to dashboard Cite

show abstract

“…One recent report suggests that the 5-HT7 receptor is involved in male courtship (Becnel et al 2011). Overexpression or elimination of one aminergic system at a time has also demonstrated possible dopaminergic effects on courtship conditioning, a learned behavior (Neckameyer 1998a), and on aberrant male-male courtship (Liu et al 2008).…”

Section: Complementary Aminergic Circuitsmentioning

confidence: 99%

Dispensable, Redundant, Complementary, and Cooperative Roles of Dopamine, Octopamine, and Serotonin inDrosophila melanogaster

Chen

Lebestky

et al. 2013

Genetics

View full text Add to dashboard Cite

To investigate the regulation of Drosophila melanogaster behavior by biogenic amines, we have exploited the broad requirement of the vesicular monoamine transporter (VMAT) for the vesicular storage and exocytotic release of all monoamine neurotransmitters. We used the Drosophila VMAT (dVMAT) null mutant to globally ablate exocytotic amine release and then restored DVMAT activity in either individual or multiple aminergic systems, using transgenic rescue techniques. We find that larval survival, larval locomotion, and female fertility rely predominantly on octopaminergic circuits with little apparent input from the vesicular release of serotonin or dopamine. In contrast, male courtship and fertility can be rescued by expressing DVMAT in octopaminergic or dopaminergic neurons, suggesting potentially redundant circuits. Rescue of major aspects of adult locomotion and startle behavior required octopamine, but a complementary role was observed for serotonin. Interestingly, adult circadian behavior could not be rescued by expression of DVMAT in a single subtype of aminergic neurons, but required at least two systems, suggesting the possibility of unexpected cooperative interactions. Further experiments using this model will help determine how multiple aminergic systems may contribute to the regulation of other behaviors. Our data also highlight potential differences between behaviors regulated by standard exocytotic release and those regulated by other mechanisms. BOTH invertebrate and mammalian behaviors undergo extensive regulation by monoamine neurotransmitters (reviewed in Joshua et al.

show abstract

“…1B, represented as red spheres). Drosophila expresses five G protein-coupled serotonin receptors, Gi/Go-coupled the 5HT-R1A and -1B, the Gq-coupled -5HT-R2A and -2B and the Gs-coupled 5HT-R7 [98][99][100][101][102].…”

Section: The Serotonergic Systemmentioning

confidence: 99%

Big Lessons from Tiny Flies: Drosophila Melanogaster as a Model to Explore Dysfunction of Dopaminergic and Serotonergic Neurotransmitter Systems

Kasture

Hummel

Sučić

et al. 2018

Preprint

View full text Add to dashboard Cite

The brain of Drosophila melanogaster is comprised of some 100,00 neurons, 127 and 80 of which are dopaminergic and serotonergic, respectively. Their activity regulates behavioral functions equivalent to those in mammals, e.g. motor activity, reward and aversion, memory formation, feeding, sexual appetite etc. Mammalian dopaminergic and serotonergic neurons are known to be heterogeneous. They differ in their projections and in their gene expression profile. A sophisticated genetic tool box is available, which allows for targeting virtually any gene with amazing precision in Drosophila melanogaster. Similarly, Drosophila genes can be replaced by their human orthologs including disease-associated alleles. Finally, genetic manipulation can be restricted to single fly neurons. This has allowed for addressing the role of individual neurons in circuits, which determine attraction and aversion, sleep and arousal, odor preference etc. Flies harboring mutated human orthologs provide models, which can be interrogated to understand the effect of the mutant protein on cell fate and neuronal connectivity. These models are also useful for proof-of-concept studies to examine the corrective action of therapeutic strategies. Finally, experiments in Drosophila can be readily scaled up to an extent, which allows for drug screening with reasonably high throughput.

show abstract

The Serotonin 5-HT7Dro Receptor Is Expressed in the Brain of Drosophila, and Is Essential for Normal Courtship and Mating

Cited by 106 publications

References 62 publications

Methods to Assay <em>Drosophila</em> Behavior

Methods to Assay <em>Drosophila</em> Behavior

Dispensable, Redundant, Complementary, and Cooperative Roles of Dopamine, Octopamine, and Serotonin inDrosophila melanogaster

Big Lessons from Tiny Flies:<em> Drosophila Melanogaster </em>as a Model to Explore Dysfunction of Dopaminergic and Serotonergic Neurotransmitter Systems

Contact Info

Product

Resources

About