The Serological Pattern in Typhus Fever. I. Epidemic1

Plotz, Harry; Bennett, B. M.; Wertman, Kenneth; Snyder, Marshall L.; Gauld, Ross L.

doi:10.1093/oxfordjournals.aje.a119192

Cited by 16 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is important to note, however, that R. rickettsii spotted fever soluble antigen analyzed by them on polyacrylamide gels was also devoid of a major protein (protein 2 in the nomenclature of this laboratory). It is possible that protein 2 may contribute to the species-specific complement-fixation antigen that is prepared by extensive washing of rickettsiae to remove soluble antigen (15). The identification of protein 4 as a peripheral protein in the cell envelope of R. prowazekii, its preliminary characterization as a glycoprotein, and its contribution to typhus soluble antigen suggest that further study is warranted to determine its role in rickettsiae-host interactions.…”

Section: Resultsmentioning

confidence: 99%

Surface proteins of typhus and spotted fever group rickettsiae

Osterman¹,

Eisemann²

1978

Infect Immun

View full text Add to dashboard Cite

Six proteins, previously established as major constituents of intact organisms were identified in cell envelopes obtained from intrinsically radiolabeled Rickettsia prowazekii. Extrinsic radioiodination of intact organisms conducted at 0.5 !LM iodide indicated that protein 4 was the most peripheral, although protein 1 also had reactive groups exposed on the surface of the organisms. A 10-fold increase in iodide concentration resulted in labeling of protein 2, and at 50 ,uM iodide, all six major proteins were radiolabeled. Similar selective labeling was not achieved with R. conorii. Analysis of both typhus and spotted fever group organisms radiolabeled with galactose suggested that carbohydrate was associated with proteins 1, 3, and 4. Typhus soluble antigen included all major proteins except protein 2, which remained attached to particulate rickettsiae after ether extraction. Protein 4 appeared to be prominent in the surface topography of R. prowazekii, was a component of soluble antigen and may have an important role in rickettsiae-host interactions.

show abstract

Section: Resultsmentioning

confidence: 99%

Surface proteins of typhus and spotted fever group rickettsiae

Osterman¹,

Eisemann²

1978

Infect Immun

View full text Add to dashboard Cite

show abstract

“…Serological procedures. Complement fixation tests were performed by the method of Plotz et al (1948) employing overnight fixation at 4 C. Murine and epidemic typhus convalescent guinea pig sera were utilized.…”

Section: Methodsmentioning

confidence: 99%

MORPHOLOGICAL, CHEMICAL, AND SEROLOGICAL STUDIES OF THE CELL WALLS OFRICKETTSIA MOOSERI

et al. 1957

View full text Add to dashboard Cite

“…The serum obtained exhibited a high complement fixing antibody titer for this particulate antigen and for crude normal yolk sac. Two units of this serum were used in a complement-fixation test patterned after that routinely employed in this laboratory (Plotz et al, 1948) using two-fold serial dilutions of aliquots of fractions from different stages in the purification process as antigen. Crude infected yolk sac frequently gave a titer of 1:10,240 while the purified rickettsial suspensions usually titered 1:40 or less.…”

mentioning

confidence: 99%

Metabolic Studies of Rickettsiae

et al. 1956

View full text Add to dashboard Cite

The present study is a continuation of our examination of the scope of intrinsic metabolic capacities of rickettsiae isolated from their host cells (Wisseman et al., 1951 and 1952). It is based on the following considerations: Bovarnick and Miller (1950) presented strong evidence for the presence of a glutamic-aspartic transaminase in Rickettsia prowazeki and Rickettsia mooseri, and some confirming evidence for the occurrence of this enzyme in R. mooseri was found by Wisseman et al. (1952). Since animal tissues (Cammarata and Cohen, 1950) and certain bacteria (Feldman and Gunsalus, 1950) exhibit a wide scope of transaminase reactions, it was conceivable that this situation might also obtain in the rickettsiae of murine typhus. Furthermore, since evidence already at hand (Bovarnick and Miller, 1950; Wisseman et al., 1952) suggests that these microorganisms possess a complex pattern of metabolism, it was considered possible that the rickettsiae could store and transmit metabolic energy through a phosphorylating mechanism and even, perhaps, perform some synthetic activities in the isolated state separated from the host cell. Exploration of these possibilities constitutes the specific subject matter of this report. MATERILS AND METHODS Ricketisial suspensions. Purified and concentrated rickettsial suspensions were prepared from the yolk sacs of embryonated eggs infected with R. mooseri by a modification2 of the method

show abstract

The Serological Pattern in Typhus Fever. I. Epidemic1

Cited by 16 publications

References 0 publications

Surface proteins of typhus and spotted fever group rickettsiae

Surface proteins of typhus and spotted fever group rickettsiae

MORPHOLOGICAL, CHEMICAL, AND SEROLOGICAL STUDIES OF THE CELL WALLS OFRICKETTSIA MOOSERI

Metabolic Studies of Rickettsiae

Contact Info

Product

Resources

About