The serodiagnosis of human hydatid disease: 2. Additional studies on selected sera using indirect haemagglutination (IHA), enzyme linked immunosorbent assay (ELISA) and defined antigen substrate spheres (DASS)

Matossian, R. M.; McLaren, Moira L.; Draper, C. C.; Bradstreet, C. M. Patricia; Dighero, Mabel W.; Kane, G. J.; Mackinlay, L. M.; Rickard, M. D.

doi:10.1017/s0022149x0000612x

Cited by 17 publications

(6 citation statements)

References 16 publications

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“…Despite the multiplicity of immunological procedures employed in the diagnosis of human hydatid disease there still exists the possibility of welcoming a new method that promises greater sensitivity and specificity (MATOSSIAN, 1977, MATOSSIAN et a/., 1979. Improvements in the potency of the antigen and the sensitivity of the method have constantly been attempted.…”

Section: A Hydatid Diseasementioning

confidence: 99%

A simplified radioimmunoassay technique for hydatid disease and human trichinosis

Matossian

1981

J. Helminthol.

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A. A comparative study of a simplified radioimmunoassay (RIA) technique with the indirect haemagglutination (IHA) test in the diagnosis of 72 patients with hydatid disease indicated the greater sensitivity of the former. Both methods had an equal specificity in 60 control patients. RIA will serve as a useful addition to tests already available in hydatid serology.B. A comparison of the (RIA) technique with the fluorescent antibody test (FAT) in the diagnosis of 33 patients with early, active trichinosis and 30 healthy controls indicated an equal sensitivity and specificity of both methods. The adoption of RIA as an easier method is recommended.This study of a simplified radioimmunoassay technique for the diagnosis of hydatid disease and trichinosis in man is presented in two sections.

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Section: A Hydatid Diseasementioning

confidence: 99%

A simplified radioimmunoassay technique for hydatid disease and human trichinosis

Matossian

1981

J. Helminthol.

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“…1989), filariasis (Rickard et al . 1984), schistosomiasis (Mattossian et al . 1979), neurocysticercosis (Lightowlers et al .…”

Section: Introductionmentioning

confidence: 99%

Diagnostic evaluation of a synthetic peptide derived from a novel antigen B subunit as related to other available peptides and native antigens used for serology of cystic hydatidosis

Barbieri

Fernández

González

et al. 1998

Parasite Immunology

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A synthetic peptide (GU4) derived from an antigen B (AgB) subunit was serologically compared with crude antigen (HCFA); immunopurified AgB and antigen 5 (Ag5), and two other synthetic peptides, for diagnosis of human cystic hydatidosis. GU4 was derived from the sequence of AgB/2, the novel AgB subunit described by us. The other two peptides: 65 (AgB mimotope) and 89-122 (Ag5 mimotope), were described by others. Antigens B and 5 showed higher diagnostic sensitivity than corresponding peptides. All sera reacting with peptides 89-122 and GU4 also reacted with 65. The latter provided three to four times higher sensitivity than the former two peptides, but 30% lower specificity. The diagnostic efficiency of AgB (82%) was higher than those of Ag5 (74%) and HCFA (71%). Interestingly, 89-122 only reacted with hydatid sera, some of which did not react with AgB. Considering positive those reacting with 89-122 or AgB, sensitivity increases from 77% (with AgB) to 82% (combined), while specificity is the same as with AgB (86%). Our results suggest that hydatid serology may be improved by: a) combining several defined antigens (including synthetic peptides), b) design of new E. granulosus-specific mimotopes, which react with the false negative sera (16/90; 18%).

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“…Such tests include that based on intradermal reaction, haemagglutina-tion assays, complement ® xation, IFAT and ELISA. The high sensitivity of the indirect haemagglutination assay (IHA) is established and documented in both diagnostic and sero± epidemiological investigations of human hydatid disease (Matossian, 1977;Matossian et al ., 1979). In camel hydatidosis, Dada et al .…”

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The seroprevalence of cystic hydatidosis in Oman

Idris

Ruppel

Gehrig-Feistel

et al. 1999

Annals of Tropical Medicine And Parasitology

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The presence of antibodies against Echinococcus granulosus in sera from 306 humans and 390 camels in Oman was investigated by indirect haemagglutination assays. Only one of the human and five of the camel sera gave positive reactions. The reactivities of the human sera were confirmed by ELISA. These results, together with isolated reports of hydatid cysts, indicate that E. granulosus is endemic in Oman, although with a low prevalence.

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The serodiagnosis of human hydatid disease: 2. Additional studies on selected sera using indirect haemagglutination (IHA), enzyme linked immunosorbent assay (ELISA) and defined antigen substrate spheres (DASS)

Cited by 17 publications

References 16 publications

A simplified radioimmunoassay technique for hydatid disease and human trichinosis

A simplified radioimmunoassay technique for hydatid disease and human trichinosis

Diagnostic evaluation of a synthetic peptide derived from a novel antigen B subunit as related to other available peptides and native antigens used for serology of cystic hydatidosis

The seroprevalence of cystic hydatidosis in Oman

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