The serine/threonine kinase Akt gene affects fecundity by reducing Juvenile hormone synthesis in Liposcelis entomophila (Enderlein)

Lu, Ting; Lu, Yujie; Wang, Lei; Liu, Zhipei; Miao, Shiyuan; Tai, Yajie; Yang, Binbin

doi:10.1016/j.pestbp.2023.105583

Cited by 2 publications

(1 citation statement)

References 64 publications

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“…Taken together, these results suggest that TaPDK may be involved in 20E signaling in T. absoluta. Furthermore, silencing of TaPDK reduced the expression of four chitin-related genes (TaCDA1, TaCHT5, TaUAP and TaCHS) and chitin content, suggesting that it may be involved in cuticle degradation during (Lu et al 2023). In this investigation, it was observed that the expression levels of three juvenile hormone-related genes (TaKr-h1, TaMet, and TaJHA) were signi cant reduced in all individuals treated with dsTaPDK.…”

Section: Discussionmentioning

confidence: 64%

Targeting TaPDK: A Promising Strategy for Effective Control of the Destructive Tomato Leaf Miner, Tuta absoluta

Yan,

Song,

Yang

et al. 2024

Preprint

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Phosphoinositide-dependent kinase PDK, a key component of the insulin signaling pathway, plays an important role in the regulation of insect growth and development. However, the functional significance of the PDK gene in Tuta absoluta, a destructive invasive tomato leaf miner, remains unknown. In this study, we identified and characterized a PDK gene (designated TaPDK) and analyzed its physiological roles in molting and reproduction of T. absoluta. Our results showed that the TaPDK expression levels were elevated in the 5- and 6-day-old pupae before ecdysis and declined immediately after eclosion. TaPDK was predominantly expressed in the head, midgut and abdomen. When the TaPDK was knockdown, the larval-pupal molting and female reproduction were severely affected. Injecting dsRNA of TaPDK into 2-day-old female pupae resulted in epidermal wrinkling and darkening in 22.40% of pupae and head abnormalities in 8.89% of pupae, ultimately leading to death. Knockdown of TaPDK significantly reduced the 20E titer and chitin content and downregulated the expression levels of genes involved in the 20E pathway and chitin metabolism. Further investigation revealed that the suppression of TaPDK impaired ovarian development, resulting in significantly reduced fecundity and hatchability. In addition, the JH titer and vitellogenin content of TaPDK-deficient females were reduced, and the expression of Vg, VgR, and JH signaling pathway genes were significantly decreased. Collectively, our results demonstrated that TaPDK regulates pupal ecdysis and female reproduction in T. absoluta, thus providing a promising molecular target for the development of RNAi-based biopesticides to control T. absoluta.

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Section: Discussionmentioning

confidence: 64%

Targeting TaPDK: A Promising Strategy for Effective Control of the Destructive Tomato Leaf Miner, Tuta absoluta

Yan,

Song,

Yang

et al. 2024

Preprint

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Response of the serine/threonine kinase AKT and phosphoinositide‐dependent kinase PDK in Frankliniella occidentalis (Thysanoptera: Thripidae) to three kinds of foods and their regulation of reproductive function

Qiu,

Huang,

Yue

et al. 2024

Insect Molecular Biology

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Frankliniella occidentalis (Pergande) is a typical omnivorous insect that feeds on host plants, pollens and mite eggs, and poses a threat to crops worldwide. The insulin signalling pathway (ISP) is a typical nutrient‐sensitive pathway that participates in the regulation of various functions in insects. Serine/threonine kinases (AKTs) and phosphoinositide‐dependent kinases (PDKs) are key components of the ISP. In this study, the FoAKT and FoPDK genes in F. occidentalis were cloned, and the effects of three foods on their expression were determined. The expression of FoAKT and FoPDK in the thrips fed on kidney bean leaves supplemented with pine pollen or mite eggs was higher than in those primarily fed on leaves alone. Meanwhile, the fecundity of thrips fed on leaves supplemented with pine pollen was highest. In addition, RNA interference‐mediated knockdown of FoAKT and FoPDK decreased vitellogenin (Vg) content and Vg expression in females, shortened ovariole length, delayed egg development and reduced fecundity and offspring hatching rates. Furthermore, the synthesis of juvenile hormone (JH) was reduced, and the contents of glucose, trehalose, glycogen and trehalase were affected. These results suggest that FoAKT and FoPDK regulate the reproduction of F. occidentalis by regulating Vg and JH production as well as carbohydrate metabolism.

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The serine/threonine kinase Akt gene affects fecundity by reducing Juvenile hormone synthesis in Liposcelis entomophila (Enderlein)

Cited by 2 publications

References 64 publications

Targeting TaPDK: A Promising Strategy for Effective Control of the Destructive Tomato Leaf Miner, Tuta absoluta

Targeting TaPDK: A Promising Strategy for Effective Control of the Destructive Tomato Leaf Miner, Tuta absoluta

Response of the serine/threonine kinase AKT and phosphoinositide‐dependent kinase PDK in Frankliniella occidentalis (Thysanoptera: Thripidae) to three kinds of foods and their regulation of reproductive function

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