The sequential seeding of epithelial and mesenchymal cells for tissue-engineered tooth regeneration

Honda, Masaki; Tsuchiya, Shunji; Sumita, Yoshinori; Sagara, Hiroshi; Ueda, Minoru

doi:10.1016/j.biomaterials.2006.09.039

Cited by 127 publications

(100 citation statements)

References 29 publications

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“…In these studies, epithelia were removed and cells dissociated enzymatically. Precursors were then amplified and associated with MSC (originated from the same tooth) in Stem cells for tooth engineering vitro in contact with biomaterials such as collagen sponges or synthetic polymers (Honda et al, 2005(Honda et al, , 2007bYoung et al, 2002). These approaches are promising for tooth formation and/or regeneration.…”

Section: Periodontal Ligament Stem Cells (Pdlsc)mentioning

confidence: 99%

“…Single cell suspensions obtained from rat, pig or mice tooth germs have been seeded onto the surface of selected biomaterials (e.g. collagen-coated polyglycolic acid, calcium phosphate material, collagen sponges) and successfully re-implanted into the omentum of immunocompromised animals (Duailibi et al, 2004;Honda et al, 2006Honda et al, , 2007aHonda et al, , 2007bHu et al, 2006;Young et al, 2002). All these reports describe the presence of both dentin and enamel.…”

Section: Association Of Epithelial and Mesenchymal Stem Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Stem cells for tooth engineering

Bluteau

Luder

Bari³

et al. 2008

eCM

166

152

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Tooth development results from sequential and reciprocal interactions between the oral epithelium and the underlying neural crest-derived mesenchyme. The generation of dental structures and/or entire teeth in the laboratory depends upon the manipulation of stem cells and requires a synergy of all cellular and molecular events that finally lead to the formation of tooth-specific hard tissues, dentin and enamel. Although mesenchymal stem cells from different origins have been extensively studied in their capacity to form dentin in vitro, information is not yet available concerning the use of epithelial stem cells. The odontogenic potential resides in the oral epithelium and thus epithelial stem cells are necessary for both the initiation of tooth formation and enamel matrix production. This review focuses on the different sources of stem cells that have been used for making teeth in vitro and their relative efficiency. Embryonic, post-natal or even adult stem cells were assessed and proved to possess an enormous regenerative potential, but their application in dental practice is still problematic and limited due to various parameters that are not yet under control such as the high risk of rejection, cell behaviour, long tooth eruption period, appropriate crown morphology and suitable colour. Nevertheless, the development of biological approaches for dental reconstruction using stem cells is promising and remains one of the greatest challenges in the dental field for the years to come.

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Section: Periodontal Ligament Stem Cells (Pdlsc)mentioning

confidence: 99%

Section: Association Of Epithelial and Mesenchymal Stem Cellsmentioning

confidence: 99%

Stem cells for tooth engineering

Bluteau

Luder

Bari³

et al. 2008

eCM

166

152

View full text Add to dashboard Cite

show abstract

“…Different matrix constituents have been used for tooth engineering (Table 1). However, collagen, under different forms, was by far the most frequently used (Honda et al, 2007;Nakao et al, 2007;Sumita et al, 2006). Collagen gel has been successfully used for dental cells encapsulation, as obviously neither interfering with the epithelial histogenesis nor with the patterning of cell differentiation (Arany et al, 2009;Nakao et al, 2007).…”

Section: Extracellular Matrix Constituentsmentioning

confidence: 99%

Tooth Organ Engineering: Biological Constraints Specifying Experimental Approaches

Kuchler‐Bopp¹,

Keller²,

Poliard³

et al. 2011

Tissue Engineering for Tissue and Organ Regeneration

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“…In 2007 Honda et al 21 developed a unique method by sequential seeding of epithelial cells and mesenchymal cells from dissociated third molar tooth buds of pig. After further growth in athymic rats, constructs were analyzed and found to form single tooth structure conforming to the natural tooth.…”

Section: Scaffold Based Techniquementioning

confidence: 99%