The sequences of nucleotides in tRNA<sup>Arg</sup><sub>III</sub> from brewer's yeast

Kuntzel, B.; Weissenbach, Jean; Dirheimer, G.

doi:10.1016/0014-5793(72)80482-4

Cited by 29 publications

(4 citation statements)

References 24 publications

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“…The sequence m'G-mZG in positions 9 and 10 has been found previously in tRNATYr from Torulopsis utilis [ 161, in tRNATV [ 171 and in tRNAt;g from yeast [5].…”

Section: Results and Discusslonsupporting

confidence: 52%

“…The extra arm has a sequence of 5 nucleotides A-G-A-hU-U. The same sequence is found in the extra arm of tRNA$ from brewer's yeast [5].…”

Section: Pi-mentioning

confidence: 62%

“…The third one, tRNA2, has the highest solubility in the organic phase. It has been isolated [4] and sequenced [5] in our laboratory. We present here the primary structure of the second one tRNAp, which we have obtained recently with a pi&cation of 80% [6].…”

Section: Introductionmentioning

confidence: 99%

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Nucleotide sequence of tRNA^Arg_II from brewer's yeast

Weissenbach¹,

Martin²,

Dirheimer³

1972

FEBS Letters

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“…The sequence m'G-mZG in positions 9 and 10 has been found previously in tRNATYr from Torulopsis utilis [ 161, in tRNATV [ 171 and in tRNAt;g from yeast [5].…”

Section: Results and Discusslonsupporting

confidence: 52%

“…The extra arm has a sequence of 5 nucleotides A-G-A-hU-U. The same sequence is found in the extra arm of tRNA$ from brewer's yeast [5].…”

Section: Pi-mentioning

confidence: 62%

See 1 more Smart Citation

Nucleotide sequence of tRNA^Arg_II from brewer's yeast

Weissenbach¹,

Martin²,

Dirheimer³

1972

FEBS Letters

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“…Reverse-phase chromatography on RPC5 columns [I51 caused a high loss of tRNAArg activity and was therefore not used for preparative purposes. tRNAArg fraction I was homogeneous and identical with tRNA$;" [16]. The activity of tRNAArg prepared by this method was increased significantly when it was renatured according to [I71 it finally accepted 1.3 nmol arginine/A,,, unit.…”

mentioning

confidence: 95%

Arginyl‐tRNA Synthetase from Brewer's Yeast

Thiebe¹

1983

European Journal of Biochemistry

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(Received August 13/0ctober 22, 1982) -EJB 5902 tRNAArg and arginyl-tRNA synthetase have been purified to homogeneity from brewer's yeast by chromatographic methods. Arginyl-tRNA synthetase is a monomeric enzyme with a molecular weight of 72000. Two active forms of the enzyme can be found, they are interconvertible. The more stable conformation is probably the natural one. Arginyl-tRNA synthetase seems to recognize arginine very specifically. No evidence for any proof-reading mechanism could be found. The steady-state mechanism is somewhat different from the types found with arginyltRNA synthetase from other sources. However, all these results are compatible with a concerted reaction. Simultaneously with the release of AMP or pyrophosphate an allosteric rearrangement occurs. This conversion seems to be determining for the reaction mechanism.Arginyl-t RNA, glu tamyl-t RN A and glut aminyl-t RN A synthetases from different organisms are of special interest because in contrast to the seventeen other aminoacyl-t RNA synthetases they catalyse the pyrophosphate/ATP exchange reaction only in the presence of the cognate tRNA [I -61. This has been an argument in favour of a mechanism without the involvement of aminoacyl adenylate as a necessary intermediate [7]. However, this problem is not yet resolved.Arginyl-tRNA synthetase from different sources have been studied [2][3][4][5]. Apparently they all function as monomers with a molecular weight of about 70000 whereas most of the other aminoacyl-tRNA synthetases are dimers or tetramers. A rather large variety of steady-state mechanism i s found with arginyl-t RNA synthetases from different organisms and no general scheme has been deduced so far. Additional data may serve to demonstrate such a principle if it exists. MATERIALS AND METHODSDEAE-cellulose DE-52 was purchased from Whatman, benzoylated DEAE-cellulose from Boehringer (Mannheim), Sepharose 4B, Sephadex (3-25, CM-Sephadex, DEAESephadex A25 and blue Dextran 2000 were from Pharmacia.4C-labelled L-amino acids were purchased from AmershamBuchler and ~-['~C]canavanine from CEA (France). Unfractionated brewer's yeast tRNA was from Roehringer (Mannheim). Adenosine 5'-[a,P-methylene]triphosphate was from Miles and arginine hydroxamate from Sigma. Previous purifications of tRNAArg from yeast included a counter-current distribution step [12]. To avoid this method an alternative procedure was developed. tRNAArg was purified from 1.5 g brewer's yeast tRNA by chromatography on a column (5 x 50 cm) of benzoylated DEAE-cellulose [I31 with a linear gradient of 750 ml each of 0.3 ml and 1.5 M NaCl in 0.01 M MgCl,, 0.05 M acetate pH 5. Two main peaks with arginyltRNA activity eluted at 0.66 1 (I) and 0.74 l(I1). Fraction I was rechromatographed on a column (1.5 x 40 cm) of benzoylated Enzyme. Arginyl-tRNA synthetase (EC 6.1.1.19).DEAE-cellulose with a linear gradient of 1 1 each of 0.1 M and 0.7 M NaCl in 0.01 M MgCl,, 0.01 M Tris, pH 7.8. tRNAArg eluted at 1.5 1. This fraction was rechromatographed on a column (1.5 x 30cm) of Seph...

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