The sequence of the gene encoding elongation factor Tu from Chlamydia trachomatis compared with those of other organisms

Cousineau, Benoit; Cerpa, Carlos; Lefebvre, Johanne; Cedergren, Robert

doi:10.1016/0378-1119(92)90006-b

Cited by 52 publications

(35 citation statements)

References 44 publications

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“…Furthermore, the two tuf genes are normally associated with characteristic flanking genes (10). The two tuf gene copies commonly encountered within gram-negative bacteria are part of either the bacterial str operon or the tRNAtufB operon (5,10,41). The arrangement of tufA in the str operon was also found in a variety of bacteria, including Thermotoga maritima, the earliest divergent bacterium sequenced so far (33), Aquifex aeolicus (11), cyanobacteria (7,24), Bacillus spp.…”

Section: Vol 182 2000 Horizontal Transfer Of the Enterococcal Tuf Gmentioning

confidence: 98%

“…No further data suggest that such a mechanism is involved in the evolution of the elongation factors. Previous studies concluded that the two copies of tuf genes in the genomes of some bacteria resulted from an ancient event of gene duplication (10,39). Moreover, a study of the tuf gene in R. prowazekii suggested that intrachromosomal recombination has taken place in the evolution of the genome of this organism (41).…”

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Evidence for Horizontal Gene Transfer in Evolution of Elongation Factor Tu in Enterococci

Boissinot

Huletsky

et al. 2000

J Bacteriol

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The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G؉C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derived from consensus sequences of the tuf gene to amplify partial tuf sequences from 17 enterococcal species and other phylogenetically related species. The amplified DNA fragments were sequenced either by direct sequencing or by sequencing cloned inserts containing putative amplicons. Two different tuf genes (tufA and tufB) were found in 11 enterococcal species, including Enterococcus avium, Enterococcus casseliflavus, Enterococcus dispar, Enterococcus durans, Enterococcus faecium, Enterococcus gallinarum, Enterococcus hirae, Enterococcus malodoratus, Enterococcus mundtii, Enterococcus pseudoavium, and Enterococcus raffinosus. For the other six enterococcal species (Enterococcus cecorum, Enterococcus columbae, Enterococcus faecalis, Enterococcus sulfureus, Enterococcus saccharolyticus, and Enterococcus solitarius), only the tufA gene was present. Based on 16S rRNA gene sequence analysis, the 11 species having two tuf genes all have a common ancestor, while the six species having only one copy diverged from the enterococcal lineage before that common ancestor. The presence of one or two copies of the tuf gene in enterococci was confirmed by Southern hybridization. Phylogenetic analysis of tuf sequences demonstrated that the enterococcal tufA gene branches with the Bacillus, Listeria, and Staphylococcus genera, while the enterococcal tufB gene clusters with the genera Streptococcus and Lactococcus. Primary structure analysis showed that four amino acid residues encoded within the sequenced regions are conserved and unique to the enterococcal tufB genes and the tuf genes of streptococci and Lactococcus lactis. The data suggest that an ancestral streptococcus or a streptococcus-related species may have horizontally transferred a tuf gene to the common ancestor of the 11 enterococcal species which now carry two tuf genes.

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Section: Vol 182 2000 Horizontal Transfer Of the Enterococcal Tuf Gmentioning

confidence: 98%

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confidence: 99%

Evidence for Horizontal Gene Transfer in Evolution of Elongation Factor Tu in Enterococci

Boissinot

Huletsky

et al. 2000

J Bacteriol

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“…The partial DNA sequence from the gene encoding a heat-shock protein (hsp65) provided strain differentiation between bacteria of the Mycobacterium aviurn complex (Swanson et al, 1997) and the partial DNA sequence of the gene sodA encoding a manganese-dependent enzyme provided identification of streptococci to species level (Poyart et al, 1998). The sequence of prokaryotic elongation factor EFlA (formerly EF-Tu) and ATP-synthase psubunit genes have also been used in phylogenetic studies (Amann et al, 1988;Cousineau et al, 1992;Ludwig et al, 1993). In all prokaryotes studied so far, the translation initiation factor 2 (IF2) is an essential protein involved in the initiation of protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2

Hedegaard

Steffensen

Nørskov‐Lauritsen

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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Nucleotide sequence analysis is increasingly being used to identify bacteria. In this work, a PCR assay based on degenerate primers was used to obtain the partial sequence of infB, the gene encoding translation initiation factor 2 (IF2), in 39 clinical isolates of different Enterobacferiaceae. The partial sequence encodes the GTP-binding domain of IF2. Together with sequences from the literature, a total of 15 species, each represented by one to seven strains, was investigated. Phylogenetic analysis yielded an evolutionary tree which had a topology similar to a tree constructed using available 165 rRNA sequences. It is concluded that the inter-species variation of the infB gene fragment is sufficient for its use in the characterization of strains that have aberrant phenotypic reactions.

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“…Phylogenetic trees based on the 16S rRNA sequences diverge less than gyrB -based trees. Other phylogenetic markers of interest are the 16S Á/23S intergenic spacer region (7,8), the infB gene (encoding the translation initiation factor 2) (9), the ATPase b-subunit (10) and the elongation factor Tu (11). Table II shows examples of sequence databases of various phylogenetic markers.…”

Section: Phylogenetic Markersmentioning

confidence: 99%

Molecular Methods for the Analysis of Gut Microbiota

Namsolleck¹,

Thiel²,

Lawson³

et al. 2004

Microbial Ecology in Health & Disease

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This review focuses on methodological approaches used to study the composition of human faecal microbiota. Gene sequencing is the most accurate tool for revealing the phylogenetic relationships between bacteria. The main application of fluorescence in situ hybridization (FISH) in both microscopy and flow cytometry is to enumerate faecal bacteria. While flow cytometry is a very fast method, FISH microscopy still has a considerably lower detection limit.

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The sequence of the gene encoding elongation factor Tu from Chlamydia trachomatis compared with those of other organisms

Cited by 52 publications

References 44 publications

Evidence for Horizontal Gene Transfer in Evolution of Elongation Factor Tu in Enterococci

Evidence for Horizontal Gene Transfer in Evolution of Elongation Factor Tu in Enterococci

Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2

Molecular Methods for the Analysis of Gut Microbiota

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