The SepOvotropin: A New Ovarian Peptide Regulating Oocyte Transport in Sepia officinalis

Zatylny, Céline; Gagnon, Jean; Boucaud‐Camou, E.; Henry, Joël

doi:10.1006/bbrc.2000.3595

Cited by 21 publications

(11 citation statements)

References 13 publications

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“…. In the cuttlefish, Sepia officinalis, the ovarian peptide content involved in the egg-laying regulation was investigated by a myotropic bioassay, using oviduct contractions as an endpoint [9][10][11]. This bioassay was also used to identify neuropeptides regulating the same egg-laying steps in S. officinalis [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…During this period, ovarian factors released by FGO inhibit the oviducal contractions and lead to the accumulation of FGO in the genital coelom [11,20]. After mating, oocytes transport is performed through the oviduct by the whole genital tract (WGT) contractions which are modulated by neuro-and ovarian peptides [9,10,12,13]. Oocytes then received two gelatinous envelopes and are fertilized by spermatozoas stored in the female's copulatory pouch [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas

Bernay

Baudy-Floc'H

Zanuttini

et al. 2006

Molecular Reproduction Devel

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For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions, we focused on the identification of putative myotropic peptides from genital products. Two experimental approaches were developed. The first one, based on a mass spectrometry screening of the male genital products, led to the identification of the tetrapeptide APGWamide. This neuropeptide was also detected in the seminal secretions of the cephalopod Sepia officinalis. In this species, APGWamide is directly involved in the oocyte transport. In Crassostrea, in vitro bioassay demonstrated that APGWamide modulates the AM contractions that insure the release of oocytes in the external medium. Exposure of oysters to a physiological concentration of APGWamide triggered repetitive shell closures. The second experimental approach was based on the monitoring of HPLC purification by a myotropic bioassay using the cuttlefish oviduct contractions as a target. The successive purification steps of the acidic extraction of ovaries from mature female oysters, led to the characterization of the hexapeptide PIESVD. When applied to mature female oysters, this peptide triggered the increase of shell closure frequency. The activity of these two regulatory peptides is the first experimental evidence of a peptidergic control of egg-laying in oyster. APGWamide and PIESVD could be used, in commercial and experimental hatcheries, for the identification of mature females to be selected for in vitro fertilization.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas

Bernay

Baudy-Floc'H

Zanuttini

et al. 2006

Molecular Reproduction Devel

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“…Ovarian regulatory peptides that stimulate oviduct and accessory sex gland contractions have already been identified in Sepia officinalis [5] [6] [7] [8] [9].…”

Section: Introductionmentioning

confidence: 99%

A Complex Set of Sex Pheromones Identified in the Cuttlefish Sepia officinalis

Enault¹,

Zatylny-Gaudin²,

Bernay

et al. 2012

PLoS ONE

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BackgroundThe cephalopod mollusk Sepia officinalis can be considered as a relevant model for studying reproduction strategies associated to seasonal migrations. Using transcriptomic and peptidomic approaches, we aim to identify peptide sex pheromones that are thought to induce the aggregation of mature cuttlefish in their egg-laying areas.ResultsTo facilitate the identification of sex pheromones, 576 5′-expressed sequence tags (ESTs) were sequenced from a single cDNA library generated from accessory sex glands of female cuttlefish. Our analysis yielded 223 unique sequences composed of 186 singletons and 37 contigs. Three major redundant ESTs called SPα, SPα′ and SPβ were identified as good candidates for putative sex pheromone transcripts and are part of the 87 unique sequences classified as unknown. The alignment of translated SPα and SPα′ revealed a high level of conservation, with 98.4% identity. Translation led to a 248-amino acid precursor containing six peptides with multiple putative disulfide bonds. The alignment of SPα-α′ with SPβ revealed a partial structural conservation, with 37.3% identity. Translation of SPβ led to a 252-amino acid precursor containing five peptides. The occurrence of a signal peptide on SPα, SPα′ and SPβ showed that the peptides were secreted. RT-PCR and mass spectrometry analyses revealed a co-localization of transcripts and expression products in the oviduct gland. Preliminary in vitro experiments performed on gills and penises revealed target organs involved in mating and ventilation.ConclusionsThe analysis of the accessory sex gland transcriptome of Sepia officinalis led to the identification of peptidic sex pheromones. Although preliminary functional tests suggested the involvement of the α3 and β2 peptides in ventilation and mating stimulation, further functional investigations will make it possible to identify the complete set of biological activities expected from waterborne pheromones.

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“…The first ovarian regulatory peptide ever characterized was the tetrapeptide ILME [4], followed by SepOvotropin [5], SepCRPs (Sepia Capsule Releasing Peptides) [6,8], and OJPs (Ovarian Jelly Peptides) [7]. All these peptides modulate the contraction of the distal oviduct, and some of them also regulate the contraction of the main nidamental glands (Figures 7B, C, and 8A-E).…”

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confidence: 99%

“…In SepCRPs, there was a mistake about the Figure 7. Perfusion of distal oviduct with (A) seawater used for incubating mature oocytes (SWO), (B) the synthetic peptide ILME and (C) synthetic SepOvotropin [4,5]. amino acid in position 5: aspartate (D) should be replaced by asparagine (N) (EISLNKDEVK instead of EISLNKD).…”

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Egg-Laying in the Cuttlefish Sepia officinalis

Zatylny-Gaudin¹,

Henry²

2018

Biological Resources of Water

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This chapter reviews studies about egg-laying in the cuttlefish Sepia officinalis. Egg masses are spawned in specific mating and spawning coastal areas where mates aggregate between April and June in the English Channel and all year long in the Mediterranean Sea. Environmental cues are clearly involved in the aggregation process, but chemical communication also plays a determining role in these complex mechanisms. The successive steps of egg-laying are orchestrated by three classes of regulatory peptides: (1) neuropeptides that integrate environmental cues, (2) ovarian regulatory peptides that modulate the activity of the genital tract, and (3) sex pheromones expressed and released by the oviduct gland. After egg-laying, embryo protection is ensured for 8-10 weeks by a multilayer capsule secreted by the accessory sex glands. The oviduct gland secretes the inner layer of the egg case. The main nidamental gland secretes the main polysaccharides and glycoproteins, such as Sepia Egg Case Proteins, involved in capsule formation and in embryo protection. The accessory nidamental gland expresses specific proteins inherent in the structural organization of the gland, and hosts symbiotic bacteria. Similarly to salivary glands, this gland secretes immune factors possibly associated with gamete and/or embryo protection.

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The SepOvotropin: A New Ovarian Peptide Regulating Oocyte Transport in Sepia officinalis

Cited by 21 publications

References 13 publications

Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas

Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas

A Complex Set of Sex Pheromones Identified in the Cuttlefish Sepia officinalis

Egg-Laying in the Cuttlefish Sepia officinalis

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