The separations using pure water as a mobile phase in liquid chromatography using polar-embedded stationary phases

Bocian, Szymon; Krzemińska, Katarzyna

doi:10.1080/17518253.2019.1576775

Cited by 19 publications

(8 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For efficient extraction of ionisable and relatively polar compounds, pH of the sample solution plays a key role. The pH of buffer or the mobile phase should be lower than two pH units below its pK a value to obtain the target analytes in their unionized forms so that they have a higher tendency to partition into the organic phase [ 42 ]. In this study, the effect of pH was investigated by varying the pH from 3 to 8.4 using orthophsphoric acid and sodium hydroxide solution.…”

Section: Resultsmentioning

confidence: 99%

Development and validation of liquid chromatography method for simultaneous determination of multiclass seven antibiotic residues in chicken tissues

et al. 2022

View full text Add to dashboard Cite

Background Antibiotics are routinely used on poultry for therapy and prevention of diseases and to enhance animal growth. The objective of this study was to develop and validate a sensitive and reliable liquid chromatography with UV detection (LC-UV) method for the simultaneous determination of seven multiclass antibiotic residues (amoxicillin, ampicillin, penicillin, sulfamethoxazole, gentamicin, ciprofloxacin, and erythromycin) in chicken tissues. Methods The liquid chromatography method with UV detection was optimized for complete separation of the seven selected antibiotic compounds with reversed phase and isocratic elution using Hypersil BDS-C18 (3 µm, 100 mm × 4 mm) column. The mobile phase consisted a ratio of 0.05 M Na2HPO4, acetonitrile and methanol (70:10:20), at UV absorption wavelength of 230 nm. The column thermostat was set at 40 °C, the mobile phase flow rate was 1 mL min−1, and the injection volume was 20 μL. Results All the seven standard compounds were eluted within 14 min. The results for: linearity, precision, sensitivity, accuracy, specificity, decision limit (CCα), detection capability (CCβ), suitability and method robustness were validated according to the criteria of Commission Decision 2002/657/EC guidelines. Calibration plot correlation coefficients ranged from 0.9983 to 0.9998 and the percent relative standard deviations for repeated analysis were below 5% indicating acceptable method precision. The limits of detection (LODs) and quantification (LOQs) ranged from 0.098–0.255 μg kg−1 to 0.297–0.574 μg kg−1, respectively. The accuracy study yielded recoveries in the ranges 98.1–107% for the pure compounds and 94.0–102% for the spiked drug free chicken tissue samples. Conclusions The method was found to be appropriate for simultaneous determination of five different classes of seven antibiotic residues in chicken tissues. Furthermore, this is the first instance for the simultaneous determination of seven multiclass, multi-residues analysis using LC-UV from chicken tissue samples. This is a cost-effective and alternative method with simple instrumentation approach for laboratories that lack highly specialized state-of-the-art instrumentation.

show abstract

Section: Resultsmentioning

confidence: 99%

Development and validation of liquid chromatography method for simultaneous determination of multiclass seven antibiotic residues in chicken tissues

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The 2 nd one is predicated on using clean water in settings of room temperature (lower than 60°C) [32]. If silica-based stationary phases are used, refer to the process as per aqueous liquid chromatography (PALC) [33].…”

Section: Pure Water As a Mobile Phasementioning

confidence: 99%

Green Analytical Chemistry to Eco-Friendly HPLC Techniques in Pharmaceutical Analysis: A Review

Abd-Elhafeez,

Arafa,

Amro

et al. 2024

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

REEN Chemistry (GC) is designing chemical processes and products to decrease the use of harmful materials, based on 12 theories established in 1998. The pharmaceutical industry faces a significant problem due to the worldwide requirement to comply with Green Analytical Chemistry (GAC) requirements. High-pressure liquid chromatography (HPLC) is a widely used process in the medicine industry, but it produces massive quantities of organic hazardous waste. Analytical techniques must be optimized for precision, sensitivity, repeatability, effortlessness, cost effectiveness, versatility, and speed. Though, other factors such as analysts' security and ecological effects are rarely considered. Although there are more publications about green chromatography techniques, there has not been much adoption of eco-friendly HPLC techniques in the pharmaceutical business. The major causes of this are the need for adapting the standard HPLC apparatus, a lack of time, knowledge, or analysts' confusion over the fulfilment of the method requirements. An overview of green approaches for liquid chromatography (LC) that may be quickly applied to traditional instruments was provided to create eco-friendly HPLC procedures for pharmaceutical analysis.

show abstract

“…However, in cases the solubility of the compounds in water is insufficient, it is necessary to use an organic solvent-water binary mixture to dissolve the compound. On the other hand, in most of the analyses conducted in a 100% water environment with the RPLC method, problems such as nonrepeating retention times (tR), and phase precipitation are observed in the RPLC column [16]. For these reasons, it is necessary to add a small amount of organic modifiers to the mobile phase to eliminate these problems [17,18].…”

Section: Introductionmentioning

confidence: 99%

A Study on the Retention Behavior of Some Imidazole Antimycotic Drugs in Acetonitrile-Water Binary Mixtures

SEÇİLMİŞ

Demıralay

Poturcu

2022

Ankara Universitesi Eczacilik Fakultesi Dergisi

View full text Add to dashboard Cite

Objective: In this study, the thermodynamic protonation constant ( 𝑝𝐾 𝑎 𝑠 𝑠) of antifungal azole derivative miconazole and tioconazole were investigated by the reversed-phase liquid chromatography (RPLC) method. Although classical stationary phases are the most commonly used column-packed materials in the analyses of these compounds by the RPLC method, the determination of the pKa value of miconazole and tioconazole with a cyano-based column has not been reported so far.Material and Method: Chromatographic behavior of the selected compounds was determined with Ultra Cyano column (Restek ® , 150×3.0 mm I.D, 3µm) which is compatible with hydrophobic compounds. The dependence of the retention times on the acetonitrile content of the mobile phase and the effect of the mobile phase pH on the chromatographic retention was determined for each compound by changing the pH in the range of 3.0-7.50. Result and Discussion: The 𝑝𝐾 𝑎 𝑠 𝑠 values were determined by a nonlinear regression program. The thermodynamic aqueous pKa ( 𝑝𝐾 𝑎 𝑤 𝑤 ) value of the studied compounds was calculated from the 𝑝𝐾 𝑎 𝑠 𝑠 value by using the macroscopic parameters (dielectric constant and mole fraction) that play an important role in the solvent properties. The calculated 𝑝𝐾 𝑎 𝑤 𝑤 values were compared with literature values.

show abstract

The separations using pure water as a mobile phase in liquid chromatography using polar-embedded stationary phases

Cited by 19 publications

References 41 publications

Development and validation of liquid chromatography method for simultaneous determination of multiclass seven antibiotic residues in chicken tissues

Development and validation of liquid chromatography method for simultaneous determination of multiclass seven antibiotic residues in chicken tissues

Green Analytical Chemistry to Eco-Friendly HPLC Techniques in Pharmaceutical Analysis: A Review

A Study on the Retention Behavior of Some Imidazole Antimycotic Drugs in Acetonitrile-Water Binary Mixtures

Contact Info

Product

Resources

About