The separation regularity of the three-phase solvent system of counter-current chromatography based on polarity parameter modeling

Jing, Jun‐Xian; Muhire, Jules; Sun, Xiaofeng; Pei, Dong; Huang, Xin‐Yi

doi:10.1016/j.chroma.2022.463319

Cited by 4 publications

(2 citation statements)

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“…The two phases are typically chosen to have different polarities and are selected based on their ability to partition the sample components between them. One of the phases is often a nonpolar organic solvent, such as hexane (Hex) or heptane, while the other is a more polar solvent, such as water or methanol [15]. The two phases are mixed in a countercurrent flow through interconnected chambers typically formed by interconnected coils or columns [16,17].…”

Section: Principles and Optimization Of MDCCCmentioning

confidence: 99%

Recent trends in multidimensional countercurrent chromatography

Muhire,

Sun,

Zhang

et al. 2024

J of Separation Science

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Countercurrent chromatography (CCC) is a potent separation approach known for its remarkable efficiency and capacity in preparation. It's applied as a substitute or combined with different chromatographic techniques, resulting in its rebranding as multidimensional CCC (MDCCC). Numerous essential mixtures from natural products contain hundreds or thousands of distinct components of importance. These mix types are too complicated to separate in any reasonable time using a single CCC dimension. However, if a multidimensional technique is utilized, where a complex mixture is separated by an initial dimension, smaller fractions of that separation are gathered. Each fraction is studied individually; complex mixes can be resolved relatively quickly. Thus, several MDCCC separation features have been studied to demonstrate their advantages, limitations, and prospective capacity to separate exceedingly complex mixtures. In this review, MDCCC aspects, including principles, multiple columns system, multilayer coil J‐type, on‐line monitoring system, and applications, have been thoroughly_explored.

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Section: Principles and Optimization Of MDCCCmentioning

confidence: 99%

Recent trends in multidimensional countercurrent chromatography

Muhire,

Sun,

Zhang

et al. 2024

J of Separation Science

Self Cite

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“…HPLC is the most commonly used tool for the screening of K values. Besides, some computational models also facilitate the search for suitable solvent systems [9][10][11][12]. However, high-performance liquid chromatography (HPLC) technology and computational models do not apply to the compounds in the same chromatographic peak.…”

Section: Introductionmentioning

confidence: 99%

Nuclear magnetic resonance‐based solvent system selection for counter‐current chromatography separation of compounds present in the same high‐performance liquid chromatography peak: Flavonoids in barley seedlings as an example

Chen,

Jia,

Zou

et al. 2023

J of Separation Science

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Partition coefficient is a key parameter for counter-current chromatography separation. High-performance liquid chromatography (HPLC) is the most commonly used tool for the screening of partition coefficients. However, HPLC technology is not applicable to the compounds present in the same chromatographic peak. Nuclear magnetic resonance (NMR) technology could easily distinguish compounds according to their characteristic absorption even if they exist in the same HPLC peak. In this study, two flavonoids present in the same HPLC peak were successfully purified by counter-current chromatography with a solvent system screened by NMR to show the great potential of NMR technology in the screening of the partition coefficient of co-efflux compounds. Through NMR screening, an optimized ethyl acetate/n-buthanol/water (7:3:10, v/v/v) system was applied in this study. As a result, two flavonoids, including 4.8 mg of 3′-methoxyl-6′''-O-feruloylsaponarin and 9.8 mg of 6′''-O-feruloylsaponarin were separated from 15 mg of the mixture. There is only one methoxy group difference between the two flavonoids. This study provides a new strategy for the screening of counter-current chromatography solvent systems and broadens the application scope of counter-current chromatography.

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