The separation of the phenotypes of phosphoglucomutase, erythrocyte acid phosphatase, and some haemoglobin variants by isoelectric focusing

Burdett, P.E.; Whitehead, Philip

doi:10.1016/0003-2697(77)90255-x

Cited by 46 publications

(12 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, it was recently elucidated by several investigators [Bark et al, 1976;Burden andWhitehead. 1977: Kiihnlctal.. 1977] that using isoelectric focusing in pol yacrylamide gel.…”

Section: Phosphoglucomutasementioning

confidence: 99%

Isoelectric Focusing Studies of Human Red Cell PGM, in Japanese, with Special Reference to the Characterization of PGM17

Nishigaki¹,

Benkmann²,

Goedde³

1982

Hum Hered

View full text Add to dashboard Cite

The distribution of phosphoglucomutase (PGM,) subtypes in human red cells was determined by isoelectric focusing in 218 Japanese samples. Nine common phenotypes were observed corresponding to the following frequencies of the four alleles at the PGM₁ locus: PGM₁¹⁺ 0.6560, PGM₁^1– 0.1170, PGM₁²⁺ 0.1674 and PGM₁^2– 0.0505. In addition, a characterization of the PGM₁⁷sallele was performed. Our results obtained in the present study revealed the possibility that the PGM₁⁷ allele may be differentiated in the two alleles of PGM₁⁷⁺ and PGM₁^7– through an investigation of isoelectric focusing.

show abstract

“…In addition, it was recently elucidated by several investigators [Bark et al, 1976;Burden andWhitehead. 1977: Kiihnlctal.. 1977] that using isoelectric focusing in pol yacrylamide gel.…”

Section: Phosphoglucomutasementioning

confidence: 99%

Isoelectric Focusing Studies of Human Red Cell PGM, in Japanese, with Special Reference to the Characterization of PGM17

Nishigaki¹,

Benkmann²,

Goedde³

1982

Hum Hered

View full text Add to dashboard Cite

show abstract

“…This latter tech nique characterized by its improved resolv ing power succeeded in the detection of a more complex PGM pattern as reported for the first time by Ishimoto and Kawata [3] and Burdett and Whitehead [2]. Bark et al [1], Sutton and Burgess [9], Kiihnl and Spielmann (5] concluded that the observed phenotypes could be explained by the exist ence of four distinct alleles at PGM, locus.…”

Section: Introductionmentioning

confidence: 80%

Isoelectric Focusing of Human Phosphoglucomutase

Vergnes¹,

Sevin²

1981

Hum Hered

View full text Add to dashboard Cite

Leukocyte lysates obtained from blood specimens of individuals from Central and Western Pyrenean groups (Barèges and Basques) and from the population of Toulouse city have been typed for PGM^a₁ isozymes using isoelectric focusing (pH range: 3–9.5) in polyacrylamide gels. The determination of usual PGM₁ phenotypes was simultaneously performed on red cells by starch gel electrophoresis. Significant differences were found between the three communities. In Barèges samples only 6 of the 10 phenotypes known at PGM^a₁ locus have been observed. In the same group, 36 mating types were studied in distinct families. The segregation on the phenotypes is in agreement with the codominant inheritance of the alleles. In Basques the gene frequencies showed intermediate values between Toulouse and Barèges. PGM^a2₁ reaches a higher frequency in the latter community (0.306). In the Toulouse population sample the results are comparable with those previously published in western Europeans.

show abstract

“…2 4 mm, PH 5-6.5) (Mittermiiller et al, 1985). AcP isoenzymes were determined by ultra-thin-layer isoelectric focusing in polyacrylamide gels (Burdet and Whitehead, 1977) with modifications (size 2 4 0~ 120X0.24 mm, PH 3.5-9.5).…”

Section: Enzyrne Analvsismentioning

confidence: 99%

Epstein‐barr virus‐positive recipient type B‐cells survive in a “complete chimera” after allogeneic bone‐marrow transplantation

Gerhartz¹,

Mittermüller

Raghavachar

et al. 1988

Intl Journal of Cancer

View full text Add to dashboard Cite

Yao et al., 1985a) or of survival of low numbers of infected B cells (Yao et al., 198%). Lymphoproliferative tumors do not usually carry EBV genomes (Andiman et al., 1983) but a few cases of EBV-positive lymphoma have been described following BMT (Schubach et al., 1985). These were monoclonal (Schubach et al., 1982), but polyclonal tumors have been described as well and all were of donor origin whenever this point was investigated (Martin et al., 1984). This, together with the observation that EBV-specific cytotoxic T cells re-emerge relatively early after allogeneic BMT (Crawford et a l . , 1986) argues against long-term persistence of infected B cells in vivo.Allogeneic BMT offers an opportunity to study this problem since either sex differences between donor and recipient or genetic polymorphism of cellular isoenzymes such as AcP or PGM-1 make it possible, in most cases, to determine whether the cells in BMT patients are of donor or recipient origin (Mittermuller et a l . , 1986). More than 90% of European adults have been infected with EBV (Yao et al., 1985~). It is an open question whether EBV-immortalized B-cell clones of the recipient persist or whether they are eliminated either by BMT conditioning or by immunological mechanisms. We report findings which suggest that EBV-transformed clones of recipient origin can indeed persist after BMT even if the patient has become a "complete chimera" according to the most sensitive criteria that are presently available. PatientA 43-year-old woman presented with severe tricytopenia following nitrefazol (anti-obesity drug) complicated by bleed- Post-grafting immunosuppression consisted of Cyclosporin A i.v. 5 mg/kg body weight for 5 days followed by 3 mg/kg until day 34 and subsequently orally until day 250 (9 months). No steroids were given at any time. At the time of writing, she is in full clinical remission (40 months). Cell preparationThe patient's bone marrow cells were aspirated with preservative-free heparin after informed consent had been obtained. For culture and cytogenetic studies the cells were centrifuged over Ficoll (density 1,077 g/l). For enzyme analyses the erythrocytes were sedimented and the leukocyte-rich buffy coat was further processed. Polymorphonuclear cells were isolated from the pellet of Ficoll separations by lysis of the contaminating erythrocytes with ammonium chloride. Thrombocytes were recovered from the upper layers of the buffy coat after mild sedimentation. T cells and monocytes were isolated in the pellet of Ficoll-gradients by a direct immune-rosetting technique (Wilhelm et al., 1986) using erythrocytes coated with pan-T-antibodies (CD2, Rieber et al., 1986) or with antimonocyte antibodies. T-depleted lymphocytes were recovered from the interface. Morphological studiesSmears were prepared by standard methods employing MayGriinwald-Giernsa stain, periodic acid Schiff reaction and myeloperoxidase. Immunological phenotypingSurface markers were identified with an enzyme-immunoassay on poly-1-lysine coated adhesive slides (Morich et al...

show abstract

The separation of the phenotypes of phosphoglucomutase, erythrocyte acid phosphatase, and some haemoglobin variants by isoelectric focusing

Cited by 46 publications

References 17 publications

Isoelectric Focusing Studies of Human Red Cell PGM, in Japanese, with Special Reference to the Characterization of PGM<sub>1</sub><sup>7</sup>

Isoelectric Focusing Studies of Human Red Cell PGM, in Japanese, with Special Reference to the Characterization of PGM<sub>1</sub><sup>7</sup>

Isoelectric Focusing of Human Phosphoglucomutase

Epstein‐barr virus‐positive recipient type B‐cells survive in a “complete chimera” after allogeneic bone‐marrow transplantation

Contact Info

Product

Resources

About