The separation of heavy meromyosin isoenzymes by differential actin binding

Trayer, Hylary R.; Winstanley, Monica A.; Trayer, Ian P.

doi:10.1016/0014-5793(77)80659-5

Cited by 24 publications

(5 citation statements)

References 19 publications

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“…Cardiac myosin was prepared from bovine ventricles by the procedure outline in [18], except that the minced muscle washed with 0.04 M KCI before extraction and after each subsequent precipitation step in order to remove the red colouration. Cardiac S 1 was prepared essentially by the procedurc i n [22] and was subsequently passed down an affinity column of Sepharose-6-aminohexan-1-01 pyrophosphate [23] to remove a specific contaminating fragment of molecular mass 35 kDa that can be observed in most published polyacrylamide gels of cardiac S1 (e. g. [22]). Actin from rabbit skeletal muscle was prepared from muscle acetone powder by the method of Spudich and Watt [24].…”

Section: Preparation Of Proteinsmentioning

confidence: 99%

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

Henry

Trayer

Brewer

et al. 1985

European Journal of Biochemistry

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Identical tripeptides of the sequence X‐Pro‐Lys, where X is an unknown blocking group, were isolated from trypsin digests of bovine cardiac alkali light chain and the LC2 light chain of rabbit fast muscle. Chemical, electrophoretic and 1H‐NMR evidence characterized X as an unusual amino acid, α‐N‐trimethylalanine (Me3Ala), which was earlier reported as the N‐terminal amino acid of the A1 alkali light chain of rabbit fast muscle [Henry et al. (1982) FEBS Lett. 144, 11–15]. The narrow line width and chemical shift position (δ= 3.23 ppm) of the –N+ ‐(CH3) protons of Me3Ala made 1H‐NMR spectroscopy a convenient method to search for this residue in other light chains. A survey of many different light chains showed that this signal was present in all vertebrate striated muscle light chains of the A1‐type (LC1, ‘essential’ light chains) and LC2‐type (‘DTNB’‐light chains, ‘phosphorylatable’ light chains) but was absent from all invertebrate muscle and vertebrate smooth muscle light chains tested. It was also absent from the vertebrate fast‐muscle‐specific A2‐type (LC3) light chains. The spectral characteristics of these signals were consistent with their having arisen from the protons of an – N+ ‐(CH3)3 grouping. Since no ɛ‐trimethyllysine could be detected in acid hydrolysates of these proteins, it appears that Me3Ala is a general feature as the N‐terminal amino acid in these light chains. 1H‐NMR studies on bovine cardiac myosin subfragment 1 (S1) showed that the Me3Ala methyl proton signal was clearly visible and that the spectrum more closely resembled that of a rabbit S1 isoenzyme, S1(A1), than S1(A2), suggesting that the 40‐residue N‐terminal segment of the alkali light chain in cardiac S1 also possesses a high segmental mobility. Addition of actin caused the same gross changes to the cardiac S1 spectrum as noted earlier for rabbit S1(A1) [Prince et al. (1981) Eur. J. Biochem. 121, 213–219]. In particular, a marked reduction in the segmental mobility of the N‐terminal region of the alkali light chain was noted, consistent with a direct interaction of this area with actin.

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Section: Preparation Of Proteinsmentioning

confidence: 99%

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

Henry

Trayer

Brewer

et al. 1985

European Journal of Biochemistry

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“…This has a direct relationship with the bearing properties of the wood member where bearing strength under loading parallel to the grain is generally higher that that under loading perpendicular to the grain. In the above equation, l m is the length of the dowel in the main member and d is the dowel diameter [19]. In steel-doweled joints, the slenderness ratio was less than or equal to 4(D ≤ 4), which indicates that the behavior of wooden joints is increasingly brittle.…”

Section: Resultsmentioning

confidence: 99%

Lateral Load Resistance of Laminated Veneer Lumber Sengonwooden Joints with Wooden Dowels

Palaeowati¹,

Awaludin²,

Siswosukarto³

2015

MST

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Analytical models for wooden-doweled joints need to be developed. The objective of this research is to determine whether the strength of wooden-doweled joints can be well predicted by equations developed for steel-doweled joints. In this experiment, various connection parameters, such as dowel diameter, species of the wooden dowel, and angle of joints, are studied. The joint specimens are loaded in a quasi-static state with four replications each. A5% offset diameter method is used to determine the lateral load resistance of the tested joints, while the predictions are evaluated by equations given in Indonesian National Standard (SNI) 7973-2013 and the European Yield Model (EYM). The results show that experimental lateral resistance is greater than that of the SNI prediction and is between the EYM prediction of elastic and the plastic conditions of the wooden dowel. Lateral load resistances given by the SNI and the elastic condition EYM are around 64.53% and 78.45% of the experimental result, respectively. In contrast to steeldoweled joints, the ductility coefficient of wooden-doweled joints increases as the dowel diameter increases. This is potentially due to the axial stiffness of the wooden dowels being relatively smaller than that of the wood member. Joint failure occurs due to plastic bearing deformation in the wood member surrounding the dowel, followed by the formation of two plastic hinges in the wooden dowel. Abstrak Tahanan Lateral Sambungan Kayu LVL Sengon dengan Alat Sambung Pasak Kayu. Sambungan kayu dengan pasak kayu perlu dikembangkan khususnya terkait dengan model analisisnya. Tujuan dari penelitian ini adalah mengetahui apakah kekuatan sambungan kayu sistem pasak kayu dapat diprediksi dengan persamaan yang dikembangkan dengan sambungan baut besi atau tidak. Pada penelitian ini, parameter kekuatan sambungan dikaji dengan melakukan variasi diameter pasak, berat jenis pasak dan sudut sambungan. Pengujian sambungan dilakukan secara quasi-statik monotonik dengan empat sampel ulangan. Metode offset 5% diameter digunakan untuk menentukan tahanan lateral sambungan eksperimen, sedangkan prediksinya dilakukan dengan persamaan SNI 7973-2013 dan EYM. Hasil penelitian menunjukkan bahwa tahanan lateral eksperimen lebih besar dibandingkan dengan prediksi SNI dan berada di antara prediksi EYM kondisi elastis dan plastis pasak kayu. Tahanan lateral prediksi SNI dan EYM elastis berturut-turtu sebesar ± 64,53% dan ±78,45% dari nilai tahanan lateral eksperimen. Berbeda dengan sambungan kayu menggunakan baut besi, nilai daktilitas sambungan kayu dengan pasak kayu meningkat dengan penggunaan diameter pasak yang lebih besar. Hal tersebut sangat mungkin karena nilai kekakuan aksial (EA) pasak kayu jauh lebih kecil daripada kekakuan aksial batang kayu yang disambung. Kegagalan sambungan terjadi berupa rusak tumpu pada kayu sambungan serta terbentuknya dua sendi plastis (titik bengkok) pada pasak kayu.

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“…In the above equation, l m is the length of the dowel in the main member and d is the dowel diameter [19]. In steel-doweled joints, the slenderness ratio was less than or equal to 4(D ≤ 4), which indicates that the behavior of wooden joints is increasingly brittle.…”

Section: Lateral Load Resistance Of Laminated Veneer Lumbermentioning

confidence: 99%

Lateral Load Resistance of Laminated Veneer Lumber Sengonwooden Joints with Wooden Dowels

Palaeowati¹,

Awaludin²,

Siswosukarto³

2015

MST

View full text Add to dashboard Cite

Analytical models for wooden-doweled joints need to be developed. The objective of this research is to determine whether the strength of wooden-doweled joints can be well predicted by equations developed for steel-doweled joints. In this experiment, various connection parameters, such as dowel diameter, species of the wooden dowel, and angle of joints, are studied. The joint specimens are loaded in a quasi-static state with four replications each. A5% offset diameter method is used to determine the lateral load resistance of the tested joints, while the predictions are evaluated by equations given in Indonesian National Standard (SNI) 7973-2013 and the European Yield Model (EYM). The results show that experimental lateral resistance is greater than that of the SNI prediction and is between the EYM prediction of elastic and the plastic conditions of the wooden dowel. Lateral load resistances given by the SNI and the elastic condition EYM are around 64.53% and 78.45% of the experimental result, respectively. In contrast to steeldoweled joints, the ductility coefficient of wooden-doweled joints increases as the dowel diameter increases. This is potentially due to the axial stiffness of the wooden dowels being relatively smaller than that of the wood member. Joint failure occurs due to plastic bearing deformation in the wood member surrounding the dowel, followed by the formation of two plastic hinges in the wooden dowel. AbstrakTahanan Lateral Sambungan Kayu LVL Sengon dengan Alat Sambung Pasak Kayu. Sambungan kayu dengan pasak kayu perlu dikembangkan khususnya terkait dengan model analisisnya. Tujuan dari penelitian ini adalah mengetahui apakah kekuatan sambungan kayu sistem pasak kayu dapat diprediksi dengan persamaan yang dikembangkan dengan sambungan baut besi atau tidak. Pada penelitian ini, parameter kekuatan sambungan dikaji dengan melakukan variasi diameter pasak, berat jenis pasak dan sudut sambungan. Pengujian sambungan dilakukan secara quasi-statik monotonik dengan empat sampel ulangan. Metode offset 5% diameter digunakan untuk menentukan tahanan lateral sambungan eksperimen, sedangkan prediksinya dilakukan dengan persamaan SNI 7973-2013 dan EYM. Hasil penelitian menunjukkan bahwa tahanan lateral eksperimen lebih besar dibandingkan dengan prediksi SNI dan berada di antara prediksi EYM kondisi elastis dan plastis pasak kayu. Tahanan lateral prediksi SNI dan EYM elastis berturut-turtu sebesar ± 64,53% dan ±78,45% dari nilai tahanan lateral eksperimen. Berbeda dengan sambungan kayu menggunakan baut besi, nilai daktilitas sambungan kayu dengan pasak kayu meningkat dengan penggunaan diameter pasak yang lebih besar. Hal tersebut sangat mungkin karena nilai kekakuan aksial (EA) pasak kayu jauh lebih kecil daripada kekakuan aksial batang kayu yang disambung. Kegagalan sambungan terjadi berupa rusak tumpu pada kayu sambungan serta terbentuknya dua sendi plastis (titik bengkok) pada pasak kayu.

show abstract

The separation of heavy meromyosin isoenzymes by differential actin binding

Cited by 24 publications

References 19 publications

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

Lateral Load Resistance of Laminated Veneer Lumber Sengonwooden Joints with Wooden Dowels

Lateral Load Resistance of Laminated Veneer Lumber Sengonwooden Joints with Wooden Dowels

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