The separation of aryl acylamidase by cross flow microfiltration and the significance of enzyme/cell debris interaction

Le, M. S.; Spark, L.B.; Ward, P.S.

doi:10.1016/s0376-7388(00)80215-x

Cited by 43 publications

(18 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Forman et al [20] presented experimental data that supported this trend, and also Sakai et al [23] observed the same phenomenon, while working with bovine blood suspension in a membrane of pore size 0.8-1.1 m. In the latter case, the critical pressure was as little as 4 kPa. It has also been reported that permeate flux may also be independent of P tm for transmembrane pressures above the critical value [24,22,25,23]. From the above it becomes evident that the effect of P tm is critical in the operation of a cross-flow filtration membrane.…”

Section: Introductionmentioning

confidence: 72%

“…Patel et al [21], found that for a system of Saccharomyces cerevisiae and a membrane with pore size 0.2 m, the P tm,crit was 50-150 kPa. Le et al [22] found that for a suspension of Pseudomonas fluorescens and a membrane with pore size of 0.2 m, the critical pressure was 100-250 kPa. Forman et al [20] presented experimental data that supported this trend, and also Sakai et al [23] observed the same phenomenon, while working with bovine blood suspension in a membrane of pore size 0.8-1.1 m. In the latter case, the critical pressure was as little as 4 kPa.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pressure effects in cross-flow microfiltration of suspensions of whole bacterial cells

Caridis

Papathanasiou

1997

Bioprocess Engineering

View full text Add to dashboard Cite

The effect of Trans-Membrane Pressure (TMP) on permeate flux during cross-flow microfiltration of bacterial cell suspensions in tubular ceramic membranes is studied experimentally. Continuous filtration experiments with suspensions of whole bacterial cells (Mycobacterium M156) show a dramatic permeate flux decline with increasing TMP. During the very early stages of the filtration process, a linear relationship between permeate flux and TMP is observed, suggesting an initial surface sorption of cells on the membrane surface. At longer times, the permeate flux vs. TMP data exhibit a critical pressure beyond which the permeate flux declines with increasing trans-membrane pressure. This is interpreted in terms of the formation of a compressible cake, whose permeability can be described through the Carman-Kozeny equation. IntroductionThe application of conventional methods (such as dead-end filtration, sedimentation, and centrifugation) for the separation of cells from liquid streams in pharmaceutical applications in a continuous production line poses many practical difficulties, mainly because of the highly hydrated and glutinous character and of the low density of biological cells. An alternative method involves the use of cross-flow membrane filtration [1] in which the filtrate passes through the membrane by virtue of a pressure differential (the TransMembrane-Pressure or TMP). In cross-flow microfiltration, the product to be filtered is passed over a tubular or flat porous surface. The flowrate of the product stream across the surface tends to maintain the direction of flow and controls the thickness of the deposited cell layer. The cell suspension to be filtered is usually pumped at a velocity in the rage 1-8 m/s parallel to the surface of the membrane and with a TMP between 0.1-0.5 MPa. The liquid permeates through the membrane and the feed emerges in a more concentrated form at the exit of the module. The partially concentrated retentate can be recycled into the reactor while the permeate is removed for further processing. Figure 1 is a schematic description of the principle behind the operation of a crossflow microfiltration membrane.While the cross-flow mode is a significant improvement over dead-end filtration, permeate flux still decreases with time, even when the feed concentration is kept constant. Typical product fluxes of 0.138 · 10\4 m3 sec\1 m\2 to 0.415 · 10\4 m3 sec\1 m\2 are observed; these do not compare very favorably to threshold flux values of between 0.2 · 10\4 m3 sec\1 m\2 and 1.11 · 10\4 m3 sec\1 m\2 which have been quoted as necessary for cross-flow microfiltration to compete with such established operations as centrifugation and precoat filtration [2,3]. Still, the advantage of continuous operation afforded by cross-flow filtration has resulted in a sustained interest for this technology. Applications of cross-flow filtration include water and sewage treatment [4], harvesting of plant and animal cells [5], food processing [6] and dewatering of mineral slurries [7]. Both submillimetre and ...

show abstract

Section: Introductionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Pressure effects in cross-flow microfiltration of suspensions of whole bacterial cells

Caridis

Papathanasiou

1997

Bioprocess Engineering

View full text Add to dashboard Cite

show abstract

“…Recovery of intracellular protein from cell lysates is accomplished by either centrifugation (Clarkson et al, 1993;Titchener-Hooker et al, 1991) or crossflow membrane filtration (Le et al, 1984a, b;Meagher et al, 1994;Quirk and Woodrow, 1984). Models that describe membrane filtration indicate that the particle size of the material being processed influences the permeate flux.…”

Section: Introductionmentioning

confidence: 99%

“…There have been studies on the operational parameters required to recover proteins from cell lysate with crossflow membrane filtration (Gabler and Ryan, 1985;Le et al, 1984a, b). Some of these studies have used E. coli cell lysate containing inclusion bodies (Forman et al, 1990;Meagher et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Crossflow microfiltration of recombinant Escherichia coli lysates after high pressure homogenization

Bailey

Meagher

1997

Biotechnol. Bioeng.

View full text Add to dashboard Cite

Crossflow membrane filtration was used to process recombinant Escherichia coli cell lysates containing protein inclusion bodies after high pressure homogenization. The number of passes through the high pressure homogenizer changed the viscosities and average particle sizes of the cell lysates. The different cell lysates were processed with a hollow fiber unit containing microfiltration membranes and a plate and frame unit with either ultrafiltration or microfiltration membranes. There were differences in permeate flux and protein transmission for the various membranes with the best performing membranes giving permeate fluxes greater than 60 L m −2 h −1 and protein transmissions greater than 90%. For a given membrane, no differences were observed between the cell lysates following homogenization with one, two, and three passes at 83 MPa. The lack of a difference between the three lysates is due to their similarities with respect to the released macromolecules and the presence of small (<0.1 µm) cell debris.

show abstract

“…One such application is the separation of protein from fermentation broths, typically isolation of yeast from Bovine Serum Albumin (BSA) protein [1] or cell/cell debris [2] from cell culture broth. Microfiltration functions on the principle of size exclusion and particles in the * Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

Performance and effects of polymeric membranes on the dead-end microfiltration of protein solution during filtration cycles

Tung

Wang

et al. 2010

Journal of Membrane Science

View full text Add to dashboard Cite

The separation of aryl acylamidase by cross flow microfiltration and the significance of enzyme/cell debris interaction

Cited by 43 publications

References 9 publications

Pressure effects in cross-flow microfiltration of suspensions of whole bacterial cells

Pressure effects in cross-flow microfiltration of suspensions of whole bacterial cells

Crossflow microfiltration of recombinant Escherichia coli lysates after high pressure homogenization

Performance and effects of polymeric membranes on the dead-end microfiltration of protein solution during filtration cycles

Contact Info

Product

Resources

About