The separation of a wide range of drugs of abuse by high-pressure liquid chromatography

Jane, I.

doi:10.1016/s0021-9673(01)80169-2

Cited by 134 publications

(9 citation statements)

References 5 publications

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“…Most of the HPLC methods previously used to assay clozapine and its metabolites in serum/plasma, have used chemically modified silica L ---(bonded phase) column packing materials and aqueous methanol or acetonitrile eluents most commonly in the pH range 2-5. As an alternative, we chose to investigate the use of unmodified (Jane, 1975;Lingeman and Undderberg, 1988;Binder et al, 1989;Law, 1990;Flanagan, 1993) and sulphopropyl modified silica (McCarthy et al, 1993;Croes et al, 1994a,b) systems with methanolic eluents containing ammonium perchlorate (pH6.7) or perchloric acid (0.01-1.0% vlv) as modifiers with the aim of injecting sample extracts directly onto the column. As a means of further varying the flexibility of the system, two chemically bonded stationary phases, cyanopropyl-modified and phenyl- modified silica were also tried with these eluents (Flanagan, 1993).…”

Section: Resultsmentioning

confidence: 99%

Measurement of clozapine and norclozapine in plasma/serum by high performance liquid chromatography with ultraviolet detection

McCarthy

Hughes

Paton³

1995

Biomedical Chromatography

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A simple method for the measurement of clozapine and its N-desmethyl metabolite in human plasma or serum by high performance liquid chromatography is described. An internal standard (aqueous nortriptyline, 4 mg/L) (50 microL) and Tris buffer (2 mol/L, pH 10.6) (100 microL) are added to plasma/serum (200 microL) and the analytes and internal standard extracted into methyl tert-butyl ether (200 microL). The extracts are analysed on a 150 mm column containing Spherisorb S5SCX using methanol containing ammonium perchlorate (35 mmol/L, pH 6.7) as eluent at a flow rate of 1.5 mL/min. Detection is by ultraviolet absorption (215 nm). The limit of detection is better than 0.05 mg/L for both analytes and the intra-assay precision (CV) for clozapine and norclozapine was 5.3 and 7.3% at 0.5 mg/L and 2.6 and 2.8% at 1.5 mg/L, respectively. The method can be applied to the measurement of these compounds in plasma after acute overdosage, for the assessment of compliance in patients apparently refractory to therapy and to identify interactions between clozapine and other neuroleptic and antidepressant drugs which may effect toxicity.

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Section: Resultsmentioning

confidence: 99%

Measurement of clozapine and norclozapine in plasma/serum by high performance liquid chromatography with ultraviolet detection

McCarthy

Hughes

Paton³

1995

Biomedical Chromatography

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“…Methanol solutions of the compounds were autoinjected by a Perkin-EImer ISS-100 automatic sampler. A modified Wheals system [14,15] was used as the mobile phase for each of the columns listed in Table 4. The ultraviolet (UV) detector was set at 254/~, a commonly used wavelength in fixed wavelength detectors.…”

Section: Methodsmentioning

confidence: 99%

The Characterization of Some 3,4-Methylenedioxyphenylisopropylamine (MDA) Analogs

Cason

1989

Journal of Forensic Sciences

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The seizure in 1986 of a large-scale clandestine laboratory producing both the N-ethyl and the N,N-dimethyl analogs of 3,4-methylenedioxyphenylisopropylamine (3,4-methylenedioxyamphetamine [MDA]) and the recent identification of N-hydroxy MDA hydrochloride (HCI) indicate an interest by illegal laboratory operators in the synthesis of noncontrolled MDA analogs. Currently, identification of these new analogs may be hampered due to lack of available standards or reference data or both. This potential problem prompted the synthesis of the following N-substituted MDA analogs: N-methyl, N-ethyl, N-propyl, N-isopropyl, N-hydroxy, and N,N-dimethyl MDA. Each compound was prepared by reductive amination of 3,4-methylenedioxyphenyl-2-propanone (MDP-2-P) using the appropriate amine hydrochloride and sodium cyanoborohydride. In addition, the acetyl derivatives of MDA, N-hydroxy MDA, and MDP-2-P oxime are reported. Spectral and chromatographic data are presented for each of the compounds synthesized.

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“…Hydrophilic interaction chromatography (HILIC) using bare silica or derivatized silica and low aqueous/high organic mobile phase has drawn the attention of LC-MS/MS practitioners. Although the name HILIC was coined by Alpert in 1990 on a derivatized silica column [2], this approach was first used by Jane in 1975 on an underivatized silica column [3]. HILIC is similar to normal phase LC in that the elution is promoted by the use of polar mobile phase.…”

Section: Introductionmentioning

confidence: 99%

Bioanalytical liquid chromatography tandem mass spectrometry methods on underivatized silica columns with aqueous/organic mobile phases

Weng

2003

Journal of Chromatography B

250

134

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The separation of a wide range of drugs of abuse by high-pressure liquid chromatography

Cited by 134 publications

References 5 publications

Measurement of clozapine and norclozapine in plasma/serum by high performance liquid chromatography with ultraviolet detection

Measurement of clozapine and norclozapine in plasma/serum by high performance liquid chromatography with ultraviolet detection

The Characterization of Some 3,4-Methylenedioxyphenylisopropylamine (MDA) Analogs

Bioanalytical liquid chromatography tandem mass spectrometry methods on underivatized silica columns with aqueous/organic mobile phases

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