The separation, long-term cultivation, and maturation of the human monocyte.

Johnson, Warren D.; Mei, Baigen; Cohn, Zanvil A.

doi:10.1084/jem.146.6.1613

Cited by 284 publications

(33 citation statements)

References 40 publications

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“…To acquire human macrophages, monocytes were isolated from human buffy coats by Ficoll density centrifugation and allowed to differentiate over 5 days in vitro to macrophages (38). Transfection of siRNA into human macrophages was performed using Viromer Blue from Origene (Rockville, MD) according to the manufacturer’s manual.…”

Section: Methodsmentioning

confidence: 99%

Oxidized LDL–bound CD36 recruits an Na ⁺ /K ⁺ -ATPase–Lyn complex in macrophages that promotes atherosclerosis

et al. 2015

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One characteristic of atherosclerosis is the accumulation of lipid-laden macrophage foam cells in the arterial wall. We have previously shown that the binding of oxidized LDL (oxLDL) to the scavenger receptor CD36 activates the kinase Lyn, initiating a cascade that inhibits macrophage migration and is necessary for foam cell generation. Here, we identified the plasma membrane ion transporter Na/K-ATPase as a key component in the macrophage oxLDL-CD36 signaling axis. Using peritoneal macrophages isolated from Atp1a1 heterozygous or Cd36 null mice, we demonstrated that CD36 recruited a Na/K-ATPase-Lyn complex for Lyn activation in response to oxLDL. Macrophages deficient in the α1 Na/K-ATPase catalytic subunit did not respond to activation of CD36, showing attenuated oxLDL uptake and foam cell formation, and oxLDL failed to inhibit migration of these macrophages. Furthermore, Apoe-null mice, which are a model of atherosclerosis, were protected from diet-induced atherosclerosis by global deletion of a single allele encoding the α1 Na+/K+-ATPase subunit or reconstitution with macrophages that lacked an allele encoding the α1 Na+/K+-ATPase subunit.. These findings identify Na/K-ATPase as a potential target for preventing or treating anti-atherosclerotic therapy.

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Section: Methodsmentioning

confidence: 99%

Oxidized LDL–bound CD36 recruits an Na ⁺ /K ⁺ -ATPase–Lyn complex in macrophages that promotes atherosclerosis

et al. 2015

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“…Macrophages were typically generated by an adherence-based method (32) in chamber slides (BD Bioscience-Falcon) with 1.75 × 10 7 PBMC per milliliter plated in 10% heat-inactivated human male AB serum (Sigma-Aldrich)/RPMI 1640 medium for 6−8 days before use. For quiescent macrophages, 10% FBS was substituted for human serum as described previously (33).…”

Section: Methodsmentioning

confidence: 99%

Editing Antigen Presentation: Antigen Transfer between Human B Lymphocytes and Macrophages Mediated by Class A Scavenger Receptors

Harvey

Quan

Rudenga

et al. 2008

The Journal of Immunology

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B lymphocytes can function independently as efficient APCs. However, our previous studies demonstrate that both dendritic cells and macrophages are necessary to propagate immune responses initiated by B cell APCs. This finding led us to identify a process in mice whereby Ag-specific B cells transfer Ag to other APCs. In this study, we report the ability and mechanism by which human B lymphocytes can transfer BCR-captured Ag to macrophages. The transfer of Ag involves direct contact between the two cells followed by the capture of B cell-derived membrane and/or intracellular components by the macrophage. These events are abrogated by blocking scavenger receptor A, a receptor involved in the exchange of membrane between APCs. Macrophages acquire greater amounts of Ag in the presence of specific B cells than in their absence. This mechanism allows B cells to amplify or edit the immune response to specific Ag by transferring BCR-captured Ag to other professional APCs, thereby increasing the frequency of its presentation. Ag transfer may perpetuate chronic autoimmune responses to specific self-proteins and help explain the efficacy of B cell-directed therapies in human disease.

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“…123,125,126 ter would interfere with the formation of antimicrobial chlorinating species from the peroxidase-H,O,-chloride interaction.66 It seems that the peroxidase in the endoplasmic reticulum is neither segregated into granules nor released into phagosomes and is, therefore, unlikely t o be involved in microbicidal events.'" Theoretically, however, the ingestion of exogenous peroxidase (e.g., from adjacent granulocytes or monocytes) could enhance the antimicrobial mechanisms of mature macrophages.66 In addition a variety of granuleassociated proteins of macrophages have been shown t o possess antimicrobial activity.…”

Section: Recognition Phagocytosis and Destruction Of Microorganismsmentioning

confidence: 99%

Macrophages: Current Views on Their Differentiation, Structure, and Function

Papadimitriou

Ashman

1989

Ultrastructural Pathology

108

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Macrophages are large mononuclear phagocytes that represent the major differentiated elements of the mononuclear phagocytic system. They arise from distinct progenitors in the bone marrow, and their immediate precursors, the monocytes, emigrate from the vascular compartment into many tissues and organs where they develop into mature macrophages. The latter display diverse morphological and functional characteristics, depending on the environmental stimuli that they receive. This phenotypic heterogeneity is, therefore, the final consequence of a series of down-regulation of some cellular processes and the up-regulation of others. The kinetics of the production of macrophages and their participation in various physiological and pathological phenomena is the subject of this review.

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The separation, long-term cultivation, and maturation of the human monocyte.

Cited by 284 publications

References 40 publications

Oxidized LDL–bound CD36 recruits an Na ⁺ /K ⁺ -ATPase–Lyn complex in macrophages that promotes atherosclerosis

Oxidized LDL–bound CD36 recruits an Na ⁺ /K ⁺ -ATPase–Lyn complex in macrophages that promotes atherosclerosis

Editing Antigen Presentation: Antigen Transfer between Human B Lymphocytes and Macrophages Mediated by Class A Scavenger Receptors

Macrophages: Current Views on Their Differentiation, Structure, and Function

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The separation, long-term cultivation, and maturation of the human monocyte.

Cited by 284 publications

References 40 publications

Oxidized LDL–bound CD36 recruits an Na + /K + -ATPase–Lyn complex in macrophages that promotes atherosclerosis

Oxidized LDL–bound CD36 recruits an Na + /K + -ATPase–Lyn complex in macrophages that promotes atherosclerosis

Editing Antigen Presentation: Antigen Transfer between Human B Lymphocytes and Macrophages Mediated by Class A Scavenger Receptors

Macrophages: Current Views on Their Differentiation, Structure, and Function

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Oxidized LDL–bound CD36 recruits an Na ⁺ /K ⁺ -ATPase–Lyn complex in macrophages that promotes atherosclerosis

Oxidized LDL–bound CD36 recruits an Na ⁺ /K ⁺ -ATPase–Lyn complex in macrophages that promotes atherosclerosis