The separation and analysis of symmetric and asymmetric dimethylarginine and other hydrophilic isobaric compounds using aqueous normal phase chromatography

Pesek, Joseph J.; Matyksa, Maria T.; Modereger, Brent A.; Hasbun, Alejandra; Phan, Vy T.; Mehr, Zahra; Watanable, Seiichiro

doi:10.1016/j.chroma.2016.02.071

Cited by 4 publications

(2 citation statements)

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“…Previous approaches to chromatographic separation of ADMA and SDMA were conducted via a normal-phase chromatography [ 20 ]. Recent paper by Pesek et al [ 21 ], presenting separation of ADMA and SDMA using aqueous normal-phase chromatography, demonstrated the use of in-source fragmentation mass spectrometric technique, in which, however, a complete separation of compounds was not achieved. In order to obtain partial separation, the authors have used an experimental AMPS column; however, the LC method was very long (30 min).…”

Section: Resultsmentioning

confidence: 99%

Quantitative Analysis of l-Arginine, Dimethylated Arginine Derivatives, l-Citrulline, and Dimethylamine in Human Serum Using Liquid Chromatography–Mass Spectrometric Method

et al. 2018

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Nitric oxide (NO) is a small molecule involved in the regulation of many physiological processes. It plays a crucial role in the regulation of nervous system, immune and inflammatory responses, and blood flow. NO is synthesized by nitric oxide synthase (NOS) during two-step oxidation of l-arginine to l-citrulline. Intermediates and derivatives of NO metabolism, such as l-arginine, l-citrulline, asymmetrical dimethylarginine (ADMA), symmetrical dimethylarginine (SDMA), and dimethylamine (DMA), are investigated as potential biomarkers. In this article, we present a novel analytical method that allowed for simultaneous analysis of l-arginine, ADMA, SDMA, l-citrulline, and DMA, in a single-step extraction and derivatization using benzoyl chloride. In brief, aliquots of serum were mixed with internal standard solution mixture (50 µM D6-DMA, 20 µM D7-ADMA, and 100 µM D7-arginine) and 0.025 M borate buffer, pH 9.2 (10:1:5). The derivatization process was performed at 25 °C for 5 min using 10% benzoyl chloride. A reverse phase column was used for chromatographic separation. Quantitation was performed using following ions (m/z): 279.1457, 286.1749, 307.1717, 314.2076, 280.1297, 150.0919, and 156.1113 for l-arginine, D7-arginine, ADMA, SDMA, D7-ADMA, l-citrulline, DMA, and D6-DMA, respectively. The method was validated, and its assay linearity, accuracy and precision, recovery, and limits of detection (1.7 µM l-arginine, 0.03 µM ADMA, 0.02 µM SDMA, 0.36 µM l-citrulline, 0.06 µM DMA) and quantification (3.2 µM l-arginine, 0.08 µM ADMA, 0.05 µM SDMA, 1.08 µM l-citrulline, 0.19 µM DMA) were determined. The method is sensitive, reliable, repeatable, and reproducible. It can be applied in the routine clinical/diagnostic laboratory.Graphical abstract

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Section: Resultsmentioning

confidence: 99%

Quantitative Analysis of l-Arginine, Dimethylated Arginine Derivatives, l-Citrulline, and Dimethylamine in Human Serum Using Liquid Chromatography–Mass Spectrometric Method

et al. 2018

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“…Very little water was adsorbed on silica hydride phases (so-called "Type C" silica) or pentafluorophenyl phases and relatively small volumes were observed on bare silica or hybrid silica. For this reason, the mechanism of interaction of silica hydride phases has sometimes been termed "aqueous normal phase chromatography" (ANP) [40][41][42] . ANP may not be a discrete separation mechanism.The term may possibly reflect a different balance of the various contributions such as adsorption, partition and ionic retention, with a greater contribution of the adsorption mechanism.…”

Section: The Water Layer On the Column Surface-does It Really Exist? mentioning

confidence: 99%

Understanding and manipulating the separation in hydrophilic interaction liquid chromatography

McCalley

2017

Journal of Chromatography A

194

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Octadecylamine and serine-derived carbon dots-modified silica gel for reversed phase/hydrophilic interaction liquid chromatography

Zhao

Yan²,

Li³

et al. 2022

Microchemical Journal

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The separation and analysis of symmetric and asymmetric dimethylarginine and other hydrophilic isobaric compounds using aqueous normal phase chromatography

Cited by 4 publications

References 40 publications

Quantitative Analysis of l-Arginine, Dimethylated Arginine Derivatives, l-Citrulline, and Dimethylamine in Human Serum Using Liquid Chromatography–Mass Spectrometric Method

Quantitative Analysis of l-Arginine, Dimethylated Arginine Derivatives, l-Citrulline, and Dimethylamine in Human Serum Using Liquid Chromatography–Mass Spectrometric Method

Understanding and manipulating the separation in hydrophilic interaction liquid chromatography

Octadecylamine and serine-derived carbon dots-modified silica gel for reversed phase/hydrophilic interaction liquid chromatography

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