The sensory-motor responses to environmental acidosis in larval zebrafish: Influences of neurotransmitter and water chemistry

JavadiEsfahani, Raha; Kwong, Raymond W. M.

doi:10.1016/j.chemosphere.2019.06.133

Cited by 10 publications

(4 citation statements)

References 33 publications

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“…However, zebrafish at 4 dpf reared at pH 4.0 are only marginally shorter (e.g. 6% shorter in the study of JavadiEsfahani and Kwong, 2019) or largely unaffected (Horng et al, 2007). Although length was not measured in the current study, larvae that were raised at pH 4.0 were slightly heavier than those raised at pH 7.6.…”

Section: Discussioncontrasting

confidence: 65%

Respirometry and cutaneous oxygen flux measurements reveal a negligible aerobic cost of ion regulation in larval zebrafish (Danio rerio)

Parker

Zimmer

Perry

2020

Journal of Experimental Biology

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Fishes living in fresh water counter the passive loss of salts by actively absorbing ions through specialized cells termed ionocytes. Ionocytes contain ATP-dependent transporters and are enriched with mitochondria; therefore ionic regulation is an energy-consuming process. The purpose of this study was to assess the aerobic costs of ion transport in larval zebrafish (Danio rerio). We hypothesized that changes in rates of Na+ uptake evoked by acidic or low Na+ rearing conditions would result in corresponding changes in whole-body oxygen consumption (ṀO2) and/or cutaneous oxygen flux (JO2), measured at the ionocyte-expressing yolk sac epithelium using the scanning micro-optrode technique (SMOT). Larvae at 4 days post-fertilization (dpf) that were reared under low pH (pH 4) conditions exhibited a higher rate of Na+ uptake compared with fish reared under control conditions (pH 7.6), yet they displayed a lower ṀO2 and no difference in cutaneous JO2. Despite a higher Na+ uptake capacity in larvae reared under low Na+ conditions, there were no differences in ṀO2 and JO2 at 4 dpf. Furthermore, although Na+ uptake was nearly abolished in 2 dpf larvae lacking ionocytes after morpholino knockdown of the ionocyte proliferation regulating transcription factor foxi3a, ṀO2 and JO2 were unaffected. Finally, laser ablation of ionocytes did not affect cutaneous JO2. Thus, we conclude that the aerobic costs of ion uptake by ionocytes in larval zebrafish, at least in the case of Na+, are below detection using whole-body respirometry or cutaneous SMOT scans, providing evidence that ion regulation in zebrafish larvae incurs a low aerobic cost.

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Section: Discussioncontrasting

confidence: 65%

Respirometry and cutaneous oxygen flux measurements reveal a negligible aerobic cost of ion regulation in larval zebrafish (Danio rerio)

Parker

Zimmer

Perry

2020

Journal of Experimental Biology

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“…In the present study, single exposure to DBDPE induced hyperactivity in zebrafish embryos/larvae, as characterized by increased frequency of tail bending at 24 hpf and increased free swimming speed under continuous light and dark/light stimulation at 144 hpf. This may be explained by increases in the contents of neurotransmitters such as GABA, Ach, Ch, and NE at 10 μg/L DBDPE exposure group, which play important roles in behavior (Tufi et al, 2016;Shi Q. et al, 2018;JavadiEsfahani and Kwong, 2019). The presence of n-TiO 2 enhanced the increase in tail bending frequencies and average speed during the first dark stimulation cycle and promoted the transcriptional levels of syn2a and α1-tubulin, while it had no effect on their protein levels.…”

Section: N-tio 2 Enhanced Dbdpe-induced Toxicity In Zebrafish Larvaementioning

confidence: 99%

Nano-TiO2 Adsorbed Decabromodiphenyl Ethane and Changed Its Bioavailability, Biotransformation and Biotoxicity in Zebrafish Embryos/Larvae

Wang

Sun

et al. 2022

Front. Environ. Sci.

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Decabromodiphenyl ethane (DBDPE), a novel brominated flame retardant, may co-exist with other pollutants including nanoparticles (NPs) in aquatic environment. Due to structural similarity with decabromodiphenyl ether, DBDPE has been reported to exhibit thyroid disrupting effects and neurotoxicity. This study further evaluated the behavior of DBDPE in aqueous environments along with the bioavailability and toxicity of DBDPE in aquatic organisms in the presence of TiO2 nanoparticles (n-TiO2). When co-existing in an aqueous environment, DBDPE was adsorbed by n-TiO2, potentially facilitating the sedimentation of DBDPE from the aqueous phase. Co-exposure to DBDPE and n-TiO2 significantly increased the uptake of DBDPE by zebrafish (Danio rerio) embryos and altered the composition of metabolites in zebrafish larvae compared to zebrafish exposed to DBDPE alone. The DBDPE-induced increases in heart rate, tail bending frequency, average speed under dark/light stimulation, and thyroid hormone levels in zebrafish embryos/larvae were further enhanced in the presence of n-TiO2. Overall, the results demonstrate that n-TiO2 affected the behavior of DBDPE in the aqueous phase and increased the bioavailability and biotoxicity of DBDPE in zebrafish embryos/larvae. These results could be helpful for understanding the environmental behavior and toxicity of DBDPE.

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“…DA is a neurotransmitter that plays a crucial role in the regulation of neural development, movement, and mood in zebrafish larvae [52]. In zebrafish, a decrease in DA content may cause neurotoxicity, presenting as a declined state of activity and unresponsiveness [53]. Azole pesticides have been demonstrated to inhibit calcium influx, leading to a reduction in DA neurotransmission [54].…”

Section: Discussionmentioning

confidence: 99%

Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos

Yang

Deng

Xing

et al. 2023

Toxics

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Difenoconazole is a type of triazole fungicide that is widely used in the treatment of plant diseases. Triazole fungicides have been shown in several studies to impair the development of the nervous system in zebrafish embryos. There is still little known about difenoconazole-induced neurotoxicity in fish. In this study, zebrafish embryos were exposed to 0.25, 0.5, and 1 mg/L of difenoconazole solution until 120 h post-fertilization (hpf). The difenoconazole-exposed groups showed concentration-dependent inhibitory tendencies in heart rate and body length. Malformation rate and spontaneous movement of zebrafish embryos increased, and the locomotor activity decreased in the highest exposure group. The content of dopamine and acetylcholine was reduced significantly in difenoconazole treatment groups. The activity of acetylcholinesterase (AChE) was also increased after treatment with difenoconazole. Furthermore, the expression of genes involved in neurodevelopment was remarkably altered, which corresponded with the alterations of neurotransmitter content and AChE activity. These results indicated that difenoconazole might affect the development of the nervous system through influencing neurotransmitter levels, enzyme activity, and the expression of neural-related genes, ultimately leading to abnormal locomotor activity in the early stages of zebrafish.

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The sensory-motor responses to environmental acidosis in larval zebrafish: Influences of neurotransmitter and water chemistry

Cited by 10 publications

References 33 publications

Respirometry and cutaneous oxygen flux measurements reveal a negligible aerobic cost of ion regulation in larval zebrafish (Danio rerio)

Respirometry and cutaneous oxygen flux measurements reveal a negligible aerobic cost of ion regulation in larval zebrafish (Danio rerio)

Nano-TiO2 Adsorbed Decabromodiphenyl Ethane and Changed Its Bioavailability, Biotransformation and Biotoxicity in Zebrafish Embryos/Larvae

Developmental Neurotoxicity of Difenoconazole in Zebrafish Embryos

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