Abstract:Context Little is known about the microbial composition of stallion semen. Aims To describe the microbiota detected in equine semen of healthy miniature pony stallions. Methods Semen specimens were collected using a Missouri artificial vagina at a single time point. PacBio (Pacific Biosciences) genomic DNA sequencing of the 16S rRNA gene was performed on these specimens, following which next-generation microbiome bioinformatics platform QIIME2 was used to process fastq files and analyse the ampli… Show more
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