The Self-diffusion Coefficients of Water and Ovalbumin in Aqueous Ovalbumin Solutions at 10°

Wang, Jui H.; Anfinsen, Christian B.; Polestra, Francesco M.

doi:10.1021/ja01648a002

Cited by 60 publications

(44 citation statements)

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“…Diffusion of water in all macromolecular solutions measured, including solutions of mucus, is slower than in pure water (as estimated by the self diffusion coefficient of water) (e.g. Wang et aL, 1954;Shephard, 1981), but the effect is closely related to the concentration of molecules in solution. Diffusion of water in all macromolecular gels studied is also slower than in water (reviewed by Muhr and Blanshard, 1982) and usually related to the polymer volume fraction of the gel.…”

Section: Unstirred Layersmentioning

confidence: 99%

Functions for fish mucus

Shephard

1994

Rev Fish Biol Fisheries

702

509

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Section: Unstirred Layersmentioning

confidence: 99%

Functions for fish mucus

Shephard

1994

Rev Fish Biol Fisheries

702

509

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“…where k© is the specific conductance of the solution, k is that of the medium without additive, and F is the volume fraction of the additive [27,29]. In pure aqueous solution, the mobility of the measured solute is 46.…”

Section: Mobility and Diffusion Coefficient In Solutions Of Differentmentioning

confidence: 99%

Capillary electrophoresis of small solutes in linear polymer solutions: Relation between ionic mobility, diffusion coefficient and viscosity

Shimizu

Kenndler

1999

Electrophoresis

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Electrophoretic mobilities, μ, and diffusion coefficients, D, of a small ion (molecular weight 579) were determined in dependence on the viscosity, η, of aqueous buffer solutions containing ethylene glycol, or polyethylene glycol (PEG) with average molecular weights of 400, 20 000, 100 000 or 2 000 000, respectively, as additives. The values for μ and D are inversely proportional to the viscosity for the solutions with small‐sized additives (ethylene glycol and PEG400), in accordance to Walden's rule. In contrast, for the longest polymers the mobilities and the diffusion coefficients approximate the values observed for pure water, and are nearly independent of the viscosity. This result agrees with the model of fractional free volume and the obstruction theory. For solutions with equal viscosity, three ranges can be differentiated for μ and D in relation to the size of the additive: for small additives, on the one hand, and the long‐chained polymers, on the other hand, the values for μ and D are nearly independent of the size of the additive. In contrast, a pronounced increase of μ and D is found with increasing poly mer size in the molecular weight range between 20 000 and 100 000. The ratio μ/D, occurring in a number of expressions for the plate height contributions, exhibits a remarkably small change over the entire polymer size and viscosity range (between 1 and 7 cP) under consideration. Consequently, the separation efficiency, expressed by the plate number, is found to be nearly constant, and is independent of viscosity.

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“…However, from the values reported for the diffusion coefficient of water in solutions of ovalbumin (Wang et al 1954), in suspensions oftobacco mosaic virus (Douglass et at. 1960) and in sodium carboxymethyl cellulose gels (Higdon & Robinson, 1962), it can be assumed that the exchange of deuterium with labile hydrogen sites in the muscles will not greatly influence the rate of diffusion of water in extracellular fluid or within the cells.…”

Section: B C Elford Permeability Of Muscle To Watermentioning

confidence: 99%

Permeation and distribution of deuterated and triated water in smooth and striated muscle

Elford¹

1970

The Journal of Physiology

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SUMMARY1. The diffusion of deuterated water (HDO) into and out of isolated guinea-pig taenia coli and frog sartorius was followed by means of a recording electronic microbalance.2. A comparison was made of the rates of efflux of deuterated and tritiated (HTO) water using the microbalance and a standard tracer technique.3. The time course of the exchange of HDO in both smooth and striated muscle was fitted to the sum of two exponential functions; however, it was not possible to correlate sizes of the intra-and extracellular spaces in the muscles with the sizes of the two compartments in model systems based on compartmental theory of tracer kinetics.4. The efflux of HDO, which was measured by means of the microbalance under stagnant conditions, occurred at a rate approximately 3 times slower than the efflux of HTO which took place while muscles were agitated vigorously in their bathing media.5. There was a marked deviation at short times from a double exponential time course during the efflux of HTO.6. Values of the tracer water permeability, Pd, of smooth and striated muscle fibres, and the extracellular space, E, of the muscles were obtained using a computer to numerically evaluate the series solutions of the equations describing the permeation of labelled water in the muscles.7. It was necessary to assume a value for the diffusion coefficient of the tracer in extracellular fluid in order to compute a unique fit to an exchange curve.

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The Self-diffusion Coefficients of Water and Ovalbumin in Aqueous Ovalbumin Solutions at 10°

Cited by 60 publications

References 1 publication

Functions for fish mucus

Functions for fish mucus

Capillary electrophoresis of small solutes in linear polymer solutions: Relation between ionic mobility, diffusion coefficient and viscosity

Permeation and distribution of deuterated and triated water in smooth and striated muscle

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