The self-assembly of spherical viruses with mixed coat proteins

Replication of Plant Viruses

“…Nucleoprotein elongation is then accomplished either by sequential addition of protein subunits (77,95) or by the addition of protein discs, fo llowed by local depolymerization to allow the subunits within the discs to fit to the end ofthe growing rod (17). Mixed reconstitution between the RNA and protein of unrelated viruses has also been reported (5,6,128,129), some of which were infective, although the mixed reconstitution of BMV and CCMV (129) must now be interpreted as reconstitution between related viruses (105). Mixed assembly of virions as a consequence of the interaction between two viruses in the same host has been reported.…”

Replication of Plant Viruses

“…Mixed virus assembly can be shown to take place in vitro between the RNA of one strain of a virus and the CP of another (Okada, 1986;Okada et al, 1970), between RNA and protein from unrelated viruses (Matthews, 1966), and between one kind of RNA and two different CPs (Wagner and Bancroft, 1968;Taliansky et al, 1977). Mixed infections are discussed by Hammond et al (1999).…”

Self-assembly of biological structures.

“…RNA in the hybrid viruses was resistant to snake-venom phosphodiesterase (81). Infectious particles, similar to reconstituted viruses, could also be made by using the RNA from one virus plus mixtures of proteins from different viruses; immunoelectrophoretic measurements suggested that particles with mixed protein coats had been formed (232). Possible reasons that the protein components of hybrid or mixed-protein viruses had relatively little effect on infectivity or on host range will be discussed below.…”