The selective oxidation of substituted aromatic hydrocarbons and the observation of uncoupling via redox cycling during naphthalene oxidation by the CYP101B1 system

Hall, Emma A.; Sarkar, Md. Raihan; Bell, Stephen

doi:10.1039/c7cy00088j

Cited by 14 publications

(14 citation statements)

References 57 publications

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“…WT CYP101B1 selectively oxidised 2,7‐dimethylnaphthalene, 1‐ and 2‐methylnaphthalene and substituted biphenyls . The substrate binding and enzymatic activity of WT and the H85F mutant of CYP101B1 were therefore tested with a range of substituent naphthalenes, biphenyls and related molecules such as phenylcyclohexane (Scheme ).…”

Section: Resultsmentioning

confidence: 99%

“…The plasmid pETDuetArx/ArR was combined in Escherichia coli with pRSFDuetArx/CYP101B1 or pRSFDuetArx/H85FCYP101B1 and used in a whole‐cell turnover to generate metabolites. , Growth and protein induction were conducted on a 100 mL scale in 2× YT medium, as described previously ,,. The cells were harvested by centrifugation (5000 g for 10 min; 8 g of cell wet weight per litre, P450 concentration ∼650 n m ) and resuspended in E .…”

Section: Methodsmentioning

confidence: 99%

“…Data for 3‐biphenylmethanol: 1 H NMR (500 MHz, CDCl 3 ): δ =7.64–7.57 (m, 3 H), 7.53 (m, 1 H), 7.64–7.57 (m, 3 H), 7.39–7.31 (m, 2 H), 4.77 ppm (s, 2 H).…”

Section: Methodsmentioning

confidence: 99%

“…Data for 2‐(7‐methylnaphthyl)methanol: 1 H NMR (500 MHz, [D 6 ]acetone): δ =7.79 (s, 1 H), 7.77 (m, 1 H), 7.75 (m, 1 H), 7.63 (s, 1 H), 7.46 (m, 1 H), 7.33 (m, 1 H), 4.77 (s, 2 H), 2.48 ppm (s, 3 H).…”

Section: Methodsmentioning

confidence: 99%

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The Oxidation of Hydrophobic Aromatic Substrates by Using a Variant of the P450 Monooxygenase CYP101B1

2017

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The cytochrome P450 monooxygenase CYP101B1, from a Novosphingobium bacterium is able to bind and oxidise aromatic substrates but at a lower activity and efficiency than norisoprenoids and monoterpenoid esters. Histidine 85 of CYP101B1 aligns with tyrosine 96 of CYP101A1, which, in the latter enzyme forms the only hydrophilic interaction with its substrate, camphor. The histidine residue of CYP101B1 was mutated to phenylalanine with the aim of improving the activity of the enzyme for hydrophobic substrates. The H85F mutant lowered the binding affinity and activity of the enzyme for β-ionone and altered the oxidation selectivity. This variant also showed enhanced affinity and activity towards alkylbenzenes, styrenes and methylnaphthalenes. For example the rate of product formation for acenaphthene oxidation was improved sixfold to 245 nmol per nmol CYP per min. Certain disubstituted naphthalenes and substrates, such as phenylcyclohexane and biphenyls, were oxidised with lower activity by the H85F variant. Variants at H85 (A and G) designed to introduce additional space into the active site so as to accommodate these larger substrates did not improve the oxidation activity. As the H85F mutant of CYP101B1 improved the oxidation of hydrophobic substrates, this residue is likely to be in the substrate binding pocket or the access channel of the enzyme. The side chain of the histidine might interact with the carbonyl groups of the favoured norisoprenoid substrates of CYP101B1.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Data for 3‐biphenylmethanol: 1 H NMR (500 MHz, CDCl 3 ): δ =7.64–7.57 (m, 3 H), 7.53 (m, 1 H), 7.64–7.57 (m, 3 H), 7.39–7.31 (m, 2 H), 4.77 ppm (s, 2 H).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Oxidation of Hydrophobic Aromatic Substrates by Using a Variant of the P450 Monooxygenase CYP101B1

2017

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“…4-alkyl 4 0 -cyanobiphenyl). In addition, phenylphenol comprising a biaryl skeleton, is used as reagent, reactant (Kikushima & Nishina, 2013;Hall et al, 2017;Yuan et al, 2017) and drug (Jiratthiya et al 2015).…”

Section: Structure Descriptionmentioning

confidence: 99%

Potassium 3-formyl-[1,1′-biphenyl]-4-olate monohydrate

2017

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The title salt, K+·C13H9O2−·H2O, was synthesized from 5-bromosalicylaldehyde and a phenylboronic acid derivative using the Suzuki–Miyaura cross-coupling reaction (Miyaura & Suzuki, 1979). In addition to the intermolecular interactions between the charged species, two O—H...O hydrogen bonds involving the isolated water molecules further stabilize the crystal packing of the title salt leading to the formation of a three-dimensional framework structure.

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Conjugative transfer of Megaplasmids pND6–1 and pND6–2 enhancing naphthalene degradation in aqueous environment: characterization and bioaugmentation prospects

Wang

et al. 2019

Appl Microbiol Biotechnol

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The selective oxidation of substituted aromatic hydrocarbons and the observation of uncoupling via redox cycling during naphthalene oxidation by the CYP101B1 system

Abstract: Oxidation of polyaromatic hydrocarbons by P450s can be lowered by redox cycling but CYP101B1 regioselectively hydroxylated substituted naphthalenes and biphenyls.

Cited by 14 publications

References 57 publications

The Oxidation of Hydrophobic Aromatic Substrates by Using a Variant of the P450 Monooxygenase CYP101B1

The Oxidation of Hydrophobic Aromatic Substrates by Using a Variant of the P450 Monooxygenase CYP101B1

Potassium 3-formyl-[1,1′-biphenyl]-4-olate monohydrate

Conjugative transfer of Megaplasmids pND6–1 and pND6–2 enhancing naphthalene degradation in aqueous environment: characterization and bioaugmentation prospects

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