The Selection Exerted by Oil Contamination on Mangrove Fungal Communities

Fasanella, Cristiane Cipola; Dias, Armando Cavalcante Franco; Rigonato, Janaína; Fiore, Marli Fátima; Soares, Fábio Lino; Melo, I. S. de; Pizzirani-Kleiner, Aline Aparecida; Elsas, Jan Dirk van; Andreote, Fernando Dini

doi:10.1007/s11270-012-1187-4

Cited by 23 publications

(14 citation statements)

References 28 publications

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“…However, the composition and dynamics of the mangrove microbiome are still scarcely explored and understood in terms of its functional groups (2,13,15,32). In our study, the qPCR data revealed statistically similar diazotrophic community abundances across the samples of the two mangroves.…”

Section: Discussionmentioning

confidence: 54%

“…Our primary purpose was to assess the effect of prior oil pollution on the diversity of these assemblages, identifying the major groups of organisms that are capable of contributing to N 2 fixation. We used samples along transects in the two mangroves that differed in vegetation composition and some environmental parameters (see below), making this survey complementary to other assessments in the same area where shifts in archaeal (10) and fungal (13) communities were demonstrated. Indeed, the abundance of genes related to nitrogen transformations and the occurrence of nifH-related genes in these areas was also observed by a deep metagenomic survey carried out by our team (2), basing our approach on determining specific players of nutrient cycling in such areas.…”

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confidence: 99%

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Abundance and Genetic Diversity of nifH Gene Sequences in Anthropogenically Affected Brazilian Mangrove Sediments

Dias

Silva

Cotta³

et al. 2012

Appl Environ Microbiol

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Although mangroves represent ecosystems of global importance, the genetic diversity and abundance of functional genes that are key to their functioning scarcely have been explored. Here, we present a survey based on the nifH gene across transects of sediments of two mangrove systems located along the coast line of São Paulo state (Brazil) which differed by degree of disturbance, i.e., an oil-spill-affected and an unaffected mangrove. The diazotrophic communities were assessed by denaturing gradient gel electrophoresis (DGGE), quantitative PCR (qPCR), and clone libraries. The nifH gene abundance was similar across the two mangrove sediment systems, as evidenced by qPCR. However, the nifH-based PCR-DGGE profiles revealed clear differences between the mangroves. Moreover, shifts in the nifH gene diversities were noted along the land-sea transect within the previously oiled mangrove. The nifH gene diversity depicted the presence of nitrogen-fixing bacteria affiliated with a wide range of taxa, encompassing members of the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and also a group of anaerobic sulfate-reducing bacteria. We also detected a unique mangrove-specific cluster of sequences denoted Mgv-nifH. Our results indicate that nitrogen-fixing bacterial guilds can be partially endemic to mangroves, and these communities are modulated by oil contamination, which has important implications for conservation strategies.

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Section: Discussionmentioning

confidence: 54%

mentioning

confidence: 99%

Abundance and Genetic Diversity of nifH Gene Sequences in Anthropogenically Affected Brazilian Mangrove Sediments

Dias

Silva

Cotta³

et al. 2012

Appl Environ Microbiol

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“…Biological nitrogen fixation is a key ecosystem process, and its activity is influenced by the resident functional microbial community and environmental conditions (13,32). In mangroves, the sediment offers an outstanding habitat in which nitrogen fixation can occur, the main reason being the oxygen gradient, including anaerobic conditions and the presence of carbonaceous and other nutrients (2,22).…”

Section: Discussionmentioning

confidence: 99%

Diversidade de arquéias e bactérias envolvidas na ciclagem do nitrogênio em sedimentos de manguezais

Dias¹

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Dedico.Ao meu amigo Fernando D. Andreote, e minha amiga Cristiane C. F. Fasanella e aos seus filhos Giovanni F. Andreote e Fellipe C. Fasanella e a Simone Raposo Cotta minha namorada e companheira. Aos amigos do laboratório em Gronigen na Holanda, onde fiz parte do meu doutorado e em especial a Michele de Cassia Pereira e Silva; Ofereço.A aluna Maryeimy Varon Lopez, orientada pelo Prof. Dr. Fernando Dini Andreote, que desenvolve projeto na mesma área, dentro do qual obteve os dados de pirosequenciamento; Á bibliotecária Marilia Garcia Henyei, pela revisão da tese; The mangrove is a coastal ecosystem, located in the interface regions between the land and sea environments, with exclusive occurrence in tropical and subtropical areas. Such location confers to the mangrove sediments unique characteristics, like as high salinity and low availability of oxygen, associated with the high abundance of organic matter. The result of these conditions is the occurrence of a restrict plant diversity, associated with a great diversity of animals, who use the mangroves for its protection and reproduction. The present study has shown how the communities of archaea (16S DNAr and amoA) and bacteria (16S DNAr and nifH) are structured in mangrove soils under distinct states of preservation. The DGGE patterns indicate alterations in the composition of targeted communities, linking its structure with the environmental contamination, while the quantifications of targeted genes by real time PCR (qPCR) has indicated alterations only in the community of ammonium oxidizers archaea. The phylogeny of these groups revealed the presence of groups commonly found in soils and water samples, besides the occurrence of particular groups, possibly resulted from a speciation process in the mangrove environment. In general, results indicated that archaeal and bacterial communities are responsive to the mangroves contamination, and its alteration can also lead to differential nitrogen processing in these soils. (BASHAN; HOLGUIN, 2002). Assim, as bactérias estão envolvidas em processos de transformação de nutrientes (e.g. fixação biológica do nitrogênio, amonificação, nitrificação e denitrificação), e também são responsáveis pela maior parte do fluxo de carbono em sedimentos de manguezais tropicais . Dessa forma, temos que nos manguezais ocorre uma interdependência entre micro e macro-organismos, onde os macro-organimos dependem dos microorganismos para a reciclagem de nutrientes, e os micro-organismos se beneficiam pela absorção de fontes de carbono oferecidas principalmente pela vegetação . É possível formular a hipótese de que na ausência ou redução da microbiota, haverá prejuízo nas atividades comerciais e ecológicas, e possivelmente a destruição dos ecossistemas de manguezais . Portanto, o conhecimento das comunidades microbianas dos 17 manguezais é de grande relevância para entender melhor a dinâmica desse ecossistema, destacando sua preservação e manutenção. Ainda dentro desta temática, alguns trabalhos têm descrito a composição de comunid...

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“…A via assimilatória é amplamente difundida em vários organismos produtores de compostos reduzidos de enxofre para biossíntese de aminoácidos compostos com S, essa via não exporta diretamente o sulfeto (BEHERA et al, 2014). A via dissimilatória é restrita para bactérias e arquéias anaeróbicas onde o sulfato ou o enxofre elementar é o aceptor final de elétrons da cadeia respiratória, produzindo grandes quantidades de sulfureto ou ácido sulfúrico (H 2 S) (BEHERA et al, 2014 VARON-LOPES et al, 2014;FASANELLA et al, 2012), que podem participar de vários processos importantes, como degradação de hidrocarbonetos, decomposição da matéria orgânica, ciclos biogeoquímicos entre outros. Essa comunidade pode ser estruturada seguindo vários fatores ambientais, entre eles as características físicas e químicas, como potencial redox, conteúdo de matéria orgânica, contaminantes orgânicos e não orgânicos (VARON-LOPES et al, 2014;SANTOS et al, 2010;XIAO-TAO 2013;SANTOS et al, 2011).…”

Section: Vias Metabólicas Envolvidas No Ciclo Do Nitrogênio E Enxofreunclassified

Descrição da comunidade microbiana ativa em solos de manguezais por metagenômica e metatranscriptômica

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The Selection Exerted by Oil Contamination on Mangrove Fungal Communities

Cited by 23 publications

References 28 publications

Abundance and Genetic Diversity of nifH Gene Sequences in Anthropogenically Affected Brazilian Mangrove Sediments

Abundance and Genetic Diversity of nifH Gene Sequences in Anthropogenically Affected Brazilian Mangrove Sediments

Diversidade de arquéias e bactérias envolvidas na ciclagem do nitrogênio em sedimentos de manguezais

Descrição da comunidade microbiana ativa em solos de manguezais por metagenômica e metatranscriptômica

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