The secretome of stem cells isolated from the adipose tissue and Wharton jelly acts differently on central nervous system derived cell populations

Abstract

IntroductionIt is hypothesized that administration of stromal/stem cells isolated from the adipose tissue (ASCs) and umbilical cord (HUCPVCs) can ameliorate the injured central nervous system (CNS). It is still not clear, however, whether they have similar or opposite effects on primary cultures of neuronal populations. The objective of the present work was to determine if ASCs and HUCPVCs preferentially act, or not, on specific cell populations within the CNS.MethodsPrimary cultures of hippocampal neurons were exposed to ASCs and HUCPVCs conditioned media (CM) (obtained 24, 48, 72 and 96 hours after three days of culture) for one week.ResultsCell viability experiments (MTS (3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2(4-sulfophenyl)-2H tetrazolium) test) revealed that CM obtained from both cell populations at all time points did not cause any deleterious effects on neuronal cells. In fact, it was determined that whenever the ASCs CM were supplemented with basic fibroblast growth factor (bFGF) and B27, there was a significant increase in the metabolic viability and neuronal cell density of the cultures. On the other hand, in the absence of CM supplementation, it was the HUCPVCs secretome that had the highest impact on the metabolic viability and cell density. In an attempt to unveil which factors could be involved in the observed effects, a screening for the presence of bFGF, nerve growth factor (NGF), stem cell factor (SCF), hepatocyte growth factors (HGF) and vascular endothelial growth factor (VEGF) in the CM was performed. Results revealed the presence of all these factors in ASCs CM, except bFGF; in contrast, in HUCPVCs CM it was only possible to detect robust NGF expression.ConclusionsOverall, the results confirm important differences on the secretome of ASCs and HUCPVCs, which lead to distinct effects on the metabolic viability and neuronal cell densities in primary cultures of hippocampal neurons; however, the factor(s) that promote the stronger effect of the HUCPVCs CM in neuronal survival is(are) still to be identified.

Combining Adult Stem Cells and Olfactory Ensheathing Cells: The Secretome Effect

“…For instance, Ribeiro et al previously reported that the secretome of bmMSCs increased the cell viability of hippocampal cultures as well as the number of neurons in culture [26]. Moreover, similar results were obtained using the conditioned medium of HUCPVCs and ASCs in neuronal and glial cultures [27,28]. To the best of our knowledge, the results presented here show for the first time the secretome interactions between OECs and ASCs, HUCPVCs, and bmMSCs on the metabolic activity and proliferation of the cells.…”

Adipose-derived stem cells decrease pain in a rat model of oxaliplatin-induced neuropathy: Role of VEGF-A modulation

“…Several studies have demonstrated that ASCs are able to efficiently differentiate toward adipogenic-chondrogenic-neurogenic-osteogenic-like cells (Quirici et al, 2010;Choudhery et al, 2013), and to modulate biomolecular signals (Gonzalez-Rey et al, 2010;Sacerdote et al, 2013;Nasef et al, 2008;Yoo et al, 2009). In particular, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and the transforming growth factor-beta 1 (TGF-b1) belong to the stem cell secretome (Ribeiro et al, 2012;Salgado et al, 2010) and these growth factors have been related to pain modulation and neuroprotection (Kiguchi et al, 2014;Maiese, 2015;Wang et al, 2015).…”

The transplantation of mesenchymal stem cells derived from unconventional sources: an innovative approach to multiple sclerosis therapy

“…Proteomic analysis of AD-MSCs secretome revealed a high content of anti-inflammatory mediators including transforming growth factor (TGF)-β, prostaglandin E2 (PGE2), and IL-10. AD-MSCs also show angiogenic properties, with recent evidence of a potential role in healing damaged tissue through secretion of vascular endothelial growth factor such as vascular endothelial growth factor (VEGF), HGF, IGF-1, platelet-derived growth factor-BB (PDGF-BB), and angiopoietin-like 4 protein (Locke et al 2009;Salgado et al 2010;Ribeiro et al 2012;Kalinina et al 2015).…”